TruSight Myeloid Sequencing Panel

This sequencing research panel targets variants associated with myeloid malignancies such as AML, MDS, MPN, CML, CMML, and JMML. Read More...
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TruSight Myeloid Sequencing Panel (96 samples)

FC-130-1010

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Accessory Products
What accessories do I need?

TruSeq Custom Amplicon Index Kit (96 indexes, 384 samples)

FC-130-1003

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TruSeq Custom Amplicon Filter Plate (1 plate)

FC-130-1006

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TruSeq Index Plate Fixture & Collar Kit (2 each)

FC-130-1007

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Product Highlights

The TruSight Myeloid Sequencing Panel uses expert-defined content and proven next-generation sequencing (NGS) technology to identify somatic variants in myeloid malignancies.

The panel content was designed by a consortium of recognized experts in blood cancer disorders and targets genes frequently mutated in:

  • Acute myeloid leukemia (AML)
  • Myelodysplastic syndrome (MDS)
  • Myeloproliferative neoplasms (MPN)
  • Chronic myelogenous leukemia (CML)
  • Chronic myelomonocytic leukemia (CMML)
  • Juvenile myelomonocytic leukemia (JMML)

The TruSight Myeloid Sequencing Panel covers 15 full genes (exons only) and 39 additional genes where oncogenic hotspots are covered, providing a comprehensive assessment of the key genes known to be involved in myeloid malignancies in a single test. The result is an accurate, cost-effective solution that enables researchers to profile liquid tumors.

View the gene list

TruSight Myeloid libraries are ideally suited to run on a desktop sequencer and perform alignment and variant calling with the MiSeq Reporter or Local Run Manager Amplicon workflow with Somatic Variant Caller. Filtering and annotation can then be performed using BaseSpace Variant Interpreter.

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Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
MiniSeq System Samples per run: mid output: 2-3, high output: 8 (based on 95% amplicons at > 500x coverage) 2 × 150 bp (max recommended)
MiSeq System 8 samples per run with v3 reagents (based on 95% amplicons at > 500x coverage) 2 × 150 bp (max recommended)
NextSeq 550 System Samples per run: mid output: 48, high output: 96 (based on 95% amplicons at > 500x coverage) 2 × 150 bp (max recommended)

Product Comparison

TruSight Myeloid Sequencing Panel AmpliSeq for Illumina Myeloid Panel AmpliSeq for Illumina Cancer Hotspot Panel v2
Assay Time 8 hours 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Cancer Type Hematologic Hematologic Pan-Cancer, Solid Tumor
Content Specifications Targets 54 genes associated with myeloid malignancies. Probes amplify 568 amplicons from tumor suppressor genes and mutational hotspots. Targets biomarkers across 69 genes (40 key DNA target genes, 29 RNA fusion driver genes, and 5 gene expression levels) Hotspot regions of 50 genes with known associations to cancer
Description For somatic mutation detection studies in myeloid malignancies. Targeted panel to study 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers. Somatic analysis research into hotspot regions of 50 cancer-related genes.
Hands-On Time 3 hours < 1.5 hours < 1.5 hours
Input Quantity 50 ng 20 ng high-quality DNA; 10 ng high-quality RNA
(10 ng recommended per pool)
1–100 ng (10 ng recommended per pool)
Method Amplicon Sequencing , Targeted DNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing , Targeted RNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing
Multiplexing Up to 96-plex Up to 96-plex 96 dual index combinations
Nucleic Acid Type DNA DNA, RNA DNA
Specialized Sample Types Not FFPE-Compatible Blood, Bone Marrow Blood, FFPE Tissue
Species Category Human Human Human

Method-Specific Workflow Example

 

Customer Stories

Looking for Cancer Clues in the Bloodstream

Next-generation sequencing has the potential to open new doors for detecting blood cancers.

Learn More
TruSight Myeloid Sequencing Panel Gene List
ABL1 CEBPA HRAS MYD88 SF3B1
ASXL1 CSF3R IDH1 NOTCH1 SMC1A
ATRX CUX1 IDH2 NPM1 SMC3
BCOR DNMT3A IKZF1 NRAS SRSF2
BCORL1 ETV6/TEL JAK2 PDGFRA STAG2
BRAF EZH2 JAK3 PHF6 TET2
CALR FBXW7 KDM6A PTEN TP53
CBL FLT3 KIT PTPN11 U2AF1
CBLB GATA1 KRAS RAD21 WT1
CBLC GATA2 MLL RUNX1 ZRSR2
CDKN2A GNAS MPL SETBP1  

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