Nextera XT DNA Library Preparation Kit

The Nextera XT DNA Library Preparation Kit allows the preparation of sequencing-ready libraries for small genomes (bacteria, archaea, viruses), amplicons, and plasmids in less than 90 minutes with only 15 minutes of hands-on time.

“We run samples from cancer and somatic genomes all using Nextera XT, all with excellent results, with a short time of hands-on work, and a very good quality of data.” —Lab Manager, Educational Institution

The industry's fastest library prep, optimized for small genomes, PCR amplicons, and plasmids

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only a single nanogram, to support a wide array of input samples. Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, Nextera XT also includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing.  Libraries prepared with Nextera XT kits are compatible with all Illumina sequencers.

PCR amplicons and small genomes can be easily prepared with Nextera XT and rapidly sequenced with a desktop sequencer. See the interview and videos below to learn more about how researchers are using Nextera to accomplish studies never before possible.

Required Accessory Products

Each Nextera XT library preparation kit (24 or 96 samples) requires one of the following Nextera XT Index kits:

  • Nextera XT Index Kit (24 indexes, 96 samples)
  • Nextera XT v2 Index Kit set A, B, C, or D (96 indexes, 384 samples each – combine all four for 384 index combinations)
  • Nextera XT Index Kit (96 indexes, 384 samples)

Sequencing Nextera XT libraries on the HiSeq 1000/2000, HiSeq 1500/2500 in High Output mode, GA, or HiScan requires one of the following sequencing primer boxes. These primers are included in the SBS reagents for the MiniSeq, MiSeq, NextSeq, or HiSeq Rapid Run mode.

  • TruSeq Dual Index Sequencing Primer Box, Single read
  • TruSeq Dual Index Sequencing Primer Box, Paired end

Featured Researchers


Dr. Thomas Sutter, Ph.D.
“Nextera XT allows our researchers to spend less time making libraries and more time making discoveries.”

—Dr. Thomas Sutter
Director of the W. Harry Feinstone Center for Genomic Research at the University of Memphis

Read Interview »




Searching for the Epigenetic Contributions of Addiction
Searching for the Epigenetic Contributions of Addiction

Targeted methylation sequencing using the MiSeq System and Nextera XT Library Preparation Kit is identifying epigenetic signatures that correlate with brain image scans of substance abusers.


Read Newsletter »


Videos

Nextera XTNextera XT DNA

Nextera XT DNA Library Prep in the Lab
See a demonstration of Nextera XT DNA Library Preparation in the lab. The fast and easy workflow allows researchers prepare sequencing-ready libraries for small genomes, PCR amplicons, and plasmids in less than 90 minutes with only 15 minutes of hands-on time.


MiSeq vs. Ion Torrent PGMMiSeq vs. Ion Torrent PGM

MiSeq vs Ion Torrent PGM in a Bacterial Research Lab
Join Dr. Tim Stinear for a presentation comparing genome sequencing of three reference bacteria of varying DNA composition with the MiSeq System using Nextera library prep against the latest PGM chemistry and chips.


Nextera XTNextera XT DNA

Nextera XT DNA Library Prep Overview
Get an overview of Nextera XT DNA Library Preparation kits, the industry's fastest and easiest method for preparing sequencing-ready libraries for small genomes, PCR amplicons, and plasmids.


Literature

Download the data sheet »

Please note that a Nextera XT Index kit is required to complete the protocol, regardless of the sample pooling level used for sequencing. Select the desired index kit using the "Add to Cart" function above.

System Compatibility

Genome Analyzer IIx

Catalog IDs: FC-131-1024, FC-131-1096, FC-131-1001, FC-131-1002, FC-121-1003, PE-121-1003, FC-131-2001, FC-131-2002, FC-131-2003, FC-131-2004