Introduction to NGS

Learn how the technology works and what it can do for you

Next-Generation Sequencing (NGS)

The massively parallel sequencing technology known as next-generation sequencing (NGS) has revolutionized the biological sciences. With its ultra-high throughput, scalability, and speed, NGS enables researchers to perform a wide variety of applications and study biological systems at a level never before possible.

Today's complex genomic research questions demand a depth of information beyond the capacity of traditional DNA sequencing technologies. Next-generation sequencing has filled that gap and become an everyday research tool to address these questions.

Welcome to NGS

NGS technology has fundamentally changed the kinds of questions scientists can ask and answer. Innovative sample preparation and data analysis options enable a broad range of applications. For example, NGS allows researchers to:

  • Rapidly sequence whole genomes
  • Zoom in to deeply sequence target regions
  • Utilize RNA sequencing (RNA-Seq) to discover novel RNA variants and splice sites, or quantify mRNAs for gene expression analysis
  • Analyze epigenetic factors such as genome-wide DNA methylation and DNA-protein interactions
  • Sequence cancer samples to study rare somatic variants, tumor subclones, and more
  • Study microbial diversity in humans or in the environment

Explore Sequencing Methods and Uses

Transitioning to NGS

Researchers start using NGS since conventional methods are too expensive and laborious. Learn how NGS enables them to identify novel cancer biomarkers missed by older methods.

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Using capillary electrophoresis-based Sanger sequencing, the Human Genome Project took over 10 years and cost nearly $3 billion.

Next-generation sequencing, in contrast, makes large-scale whole-genome sequencing (WGS) accessible and practical for the average researcher. It enables scientists to analyze the entire human genome in a single sequencing experiment, or sequence thousands to tens of thousands of genomes in one year.

Learn More About WGS

NGS Data Analysis Tools

Explore user-friendly tools designed to make data analysis accessible to any researcher, regardless of bioinformatics experience.

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NGS makes sequence-based gene expression analysis a “digital” alternative to analog techniques. It lets you quantify RNA expression with the breadth of a microarray and the resolution of qPCR.

Microarray gene expression measurement is limited by noise at the low end and signal saturation at the high end. In contrast, next-gen sequencing quantifies discrete, digital sequencing read counts, offering a broader dynamic range.

Compare Arrays vs. RNA-Seq

NGS Proves Invaluable for Biomarker Studies

Learn how researchers utilize RNA-Seq, ChIP-Seq, and exome sequencing for cancer gene expression analysis and biomarker discovery.

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Targeted sequencing allows you to sequence a subset of genes or specific genomic regions of interest, efficiently and cost-effectively focusing the power of NGS. 

NGS is highly scalable, allowing you to tune the level of resolution to meet specific experimental needs. Choose whether to do a shallow scan across multiple samples, or sequence at greater depth with fewer samples to find rare variants in a given region.

Learn More About Targeted Sequencing

 

Efficient Variant Discovery

Targeted sequencing enables researchers to uncover novel variants associated with metabolic and neurological disorders.

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Illumina sequencing utilizes a fundamentally different approach from the classic Sanger chain-termination method. It leverages sequencing by synthesis (SBS) technology – tracking the addition of labeled nucleotides as the DNA chain is copied – in a massively parallel fashion.

Next-generation sequencing generates masses of DNA sequence data that's richer and more complete than is imaginable with Sanger sequencing. Illumina sequencing systems can deliver data output ranging from 300 kilobases up to multiple terabases in a single run, depending on instrument type and configuration.

Learn More About SBS

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In-Depth NGS Introduction Introduction

In-Depth NGS Introduction

This detailed overview of Illumina sequencing describes the evolution of genomic science, major advances in sequencing technology, key methods, the basics of Illumina sequencing chemistry, and more.

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Recent Illumina next-generation sequencing technology breakthroughs include:

  • 1-channel SBS: The iSeq 100 System combines a complementary metal-oxide semiconductor chip with one-channel SBS to deliver high-accuracy data in a compact system. 
  • 2-channel SBS: This technology enables faster sequencing than the prior version of SBS technology, with the same high data accuracy.
  • Patterned flow cell technology: This option offers an exceptional level of throughput for diverse sequencing applications
  • Up to 6 terabases (Tb): Learn how the NovaSeq 6000 System offers tunable output of up to 6 Tb in ~2 days*.

*This specification is based on a dual flow cell run of S4 flow cells which have not been released; therefore, performance metrics are subject to change.

History of Illumina Sequencing

Find out how Illumina SBS technology originated and evolved over time.

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The resources below offer valuable guidance to researchers who are considering purchasing an NGS system.

Find the Right NGS Platform

Use our interactive platform selection tool to identify the right sequencer for your needs.

Launch Tool
Methods Guide

Access the information you need—from BeadChips to library preparation for genome, transcriptome, or epigenome studies to sequencer selection, analysis, and support—all in one place. Select the best tools for your lab with our comprehensive guide designed specifically for research applications.

Access Guide
Methods Guide

Explore the information below, and get help planning your experiments.

Publication Reviews

These reviews highlight some of the key ways NGS technology is furthering scientific research, in areas ranging from gene editing to single-cell analysis, cell biology, microbiology, and more.

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