Identify coding variants up to 70% faster than any other method
The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.
Extend coverage to UTRs and miRNA with Expanded Exome
Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.
Add on custom content to make Nextera Rapid Capture Exome even more powerful
Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More »
Leveraging the Illumina TruSeq family's proven data quality, new exome sequencing solutions are compatible with a variety of sample types, including FFPE, and deliver a seamless workflow, from sample to push-button data analysis.
|Metric||Nextera Rapid Capture Exome||TruSeq Rapid Exome||TruSeq Exome|
|DNA input||50 ng||50 ng||100 ng|
|Sample types||DNA||DNA||DNA and FFPE|
|Hands-on time||5 hours||3 hours||6 hours|
|Total assay time||1.5 days||1 day||2.5 days|
|Hyb time||16 hours||2 hours||16 hours|
|% coverage at 20x at 8Gb||80%||88%||90%|
“Our experience with the Nextera Rapid Capture Exome kit has been very positive.”
—ANNALISA ASTOLFI, Ph.D.
Lab Manager, Centro Interdipartimentale di Ricerche sul Cancro "Giorgio Prodi" (CIRC), University of Bologna, Italy
Read the article »
“Tools like the Nextera Rapid Capture Exome kit are allowing researchers to get a whole new view of tumors and their genetic diversity and complexity.”
—NICHOLAS NAVIN, Ph.D.
Assistant Professor in the Department of Genetics, University of Texas MD Anderson Cancer Center, Houston, Texas
Read the case study »
Nextera Rapid Capture Exome and HiSeq 2500
To help you select the most appropriate kit for your research, this table details the differences in coverage provided by Nextera Rapid Capture Exome and Expanded Exome.
Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data »
|Description||Document Type||File Info|
|Nextera Rapid Capture Exome Data Sheet,Nextera Rapid Capture Exomes||Data Sheet||PDF(< 1 MB)|
|Read Length and Nextera Rapid Capture Exome Data||Data Sheet||PDF(< 1 MB)|
Catalog IDs: FC-140-1000, FC-140-1001, FC-140-1002, FC-140-1003, FC-140-1083, FC-140-1086, FC-140-1089, FC-140-1004, FC-140-1005, FC-140-1006