Nextera Rapid Capture Exome and Expanded Exome Kits

Identify coding variants up to 70% faster than any other method
The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome
Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Add on custom content to make Nextera Rapid Capture Exome even more powerful
Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More »

Introducing TruSeq Exome and TruSeq Rapid Exome library prep kits

Leveraging the Illumina TruSeq family's proven data quality, new exome sequencing solutions are compatible with a variety of sample types, including FFPE, and deliver a seamless workflow, from sample to push-button data analysis.

Metric Nextera Rapid Capture Exome TruSeq Rapid Exome TruSeq Exome
DNA input 50 ng 50 ng 100 ng
Sample types DNA DNA DNA and FFPE
Hands-on time 5 hours 3 hours 6 hours
Total assay time 1.5 days 1 day 2.5 days
Hyb time 16 hours 2 hours 16 hours
On-target % 50% 75% 80%
% coverage at 20x at 8Gb 80% 88% 90%

Featured Researchers

Annalisa Astolfi, Ph.D.“Our experience with the Nextera Rapid Capture Exome kit has been very positive.”
—ANNALISA ASTOLFI, Ph.D.
Lab Manager, Centro Interdipartimentale di Ricerche sul Cancro "Giorgio Prodi" (CIRC), University of Bologna, Italy
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Nicholas Navin, Ph.D.“Tools like the Nextera Rapid Capture Exome kit are allowing researchers to get a whole new view of tumors and their genetic diversity and complexity.”
—NICHOLAS NAVIN, Ph.D.
Assistant Professor in the Department of Genetics, University of Texas MD Anderson Cancer Center, Houston, Texas
Read the case study »


Video

Nextera Rapid Capture Exome and Expanded Exome KitsVNextera Rapid Capture Exome and Expanded Exome Kits

Nextera Rapid Capture Exome and HiSeq 2500


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Coverage Details

Nextera Rapid Capture Exome Coverage Details

To help you select the most appropriate kit for your research, this table details the differences in coverage provided by Nextera Rapid Capture Exome and Expanded Exome.

Data

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data »

 

Product Literature

Description Document Type File Info
Nextera Rapid Capture Exome Data Sheet,Nextera Rapid Capture Exomes Data Sheet PDF< 1 MB
Read Length and Nextera Rapid Capture Exome Data Data Sheet PDF< 1 MB

Catalog IDs: FC-140-1000, FC-140-1001, FC-140-1002, FC-140-1003, FC-140-1083, FC-140-1086, FC-140-1089, FC-140-1004, FC-140-1005, FC-140-1006