Archival formalin-fixed paraffin-embedded (FFPE) samples hold an abundance of invaluable information for human cancer studies. Because these samples generally yield highly degraded DNA, they typically perform poorly in array-based applications such as whole-genome genotyping. The Infinium HD FFPE DNA Restore Kit can repair these degraded DNA samples in preparation for use with the array-based Infinium Assay.
After DNA extraction using one of many commercially available kits, the Illumina FFPE QC Kit evaluates the quality of prospective DNA samples to determine if they are viable. The QC Kit uses a real-time PCR assay that can be carried out using standard instrumentation and reagents purchased from an authorized vendor. Each kit provides primers and DNA template for 384 reactions. Extracted FFPE samples that pass the QC test are then eligible for restoration using the Infinium HD FFPE DNA Restore Kit.
Infinium FFPE QC and DNA Restoration Kits are designed to be used with Infinium BeadArray microarrays. The FFPE DNA Restore Kit is compatible with samples from any species, whereas the FFPE QC Kit is only compatible with human samples.
|Description||Document Type||File Info|
|FFPE DNA Restoration Solution Data Sheet||Data Sheet||PDF< 1 MB|
|Analyzing FFPE Samples with Infinium Microarrays||Technical Note||PDF< 1 MB|
Catalog IDs: WG-321-1001, WG-321-1002