TruSeq Stranded mRNA Library Prep Kit

The TruSeq Stranded mRNA Library Preparation Kit provides the clearest and most complete view of the transcriptome with a streamlined, cost-efficient, and scalable solution for mRNA analysis. Get precise measurement of mRNA strand orientation for detection of antisense transcription, enhanced transcript annotation, and increased alignment efficiency. High coverage uniformity enhances the discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

NeoPrep System enhances TruSeq Stranded mRNA performance

The NeoPrep System enhances TruSeq Stranded mRNA performance by providing high-quality reproducible results, even with low input amounts of RNA. Digital microfluidics technology precisely manipulates droplets that perform the library prep workflow, including quantification and normalization within the tightly controlled environment of the NeoPrep library card. A simple, intuitive workflow delivers 16 sequencing-ready libraries, eliminating almost all manual steps, and reducing hands-on time from ~4.5 hours to just 30 minutes. In addition, digital microfluidics requires less RNA input, enabling excellent performance from as little as 25 ng of total RNA.

The TruSeq Stranded mRNA Library Prep Kit low-throughput configuration includes two sets of 48 samples/12 indexes, and the high-throughput configuration includes 96 samples/96 indexes.

Interested in stranded total RNA library prep? View the TruSeq Stranded Total RNA Library Preparation Kit.

Get more information, protocols, and references on the RNA-Seq Resources Page.

Limited Use Label License:  This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc.  The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity).  The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product.  The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.

System Compatibility

Genome Analyzer IIx

Product Literature

Description Document Type File Info
TruSeq Stranded mRNA Library Prep Kit for NeoPrep Data Sheet PDF(< 1 MB)
TruSeq Stranded mRNA and Total RNA Data Sheet PDF(8 MB)

Catalog IDs: RS-122-2101, RS-122-2102, RS-122-2103, NP-202-1001