Nextera XT DNA Library Preparation Kit

Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement.Read More...
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Library Prep

Nextera XT DNA Library Preparation Kit (24 samples)

FC-131-1024

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Nextera XT DNA Library Preparation Kit (96 samples)

FC-131-1096

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Illumina Advantage Products

TG Nextera® XT DNA Sample Preparation Kit (96 Samples)

TG-131-1096

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TG Nextera® XT Index Kit v2 Set A (96 Indices, 384 Samples)

TG-131-2001

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TG Nextera® XT Index Kit v2 Set B (96 Indices, 384 Samples)

TG-131-2002

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Index Adapters

IDT® for Illumina Nextera DNA Unique Dual Indexes Set A (96 Indexes, 96 Samples)

20027213

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IDT® for Illumina Nextera DNA Unique Dual Indexes Set B (96 Indexes, 96 Samples)

20027214

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IDT® for Illumina Nextera DNA Unique Dual Indexes Set C (96 Indexes, 96 Samples)

20027215

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IDT® for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes, 96 Samples)

20027216

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Nextera XT Index Kit v2 Set A (96 indexes, 384 samples)

FC-131-2001

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Nextera XT Index Kit v2 Set B (96 indexes, 384 samples)

FC-131-2002

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Nextera XT Index Kit v2 Set C (96 indexes, 384 samples)

FC-131-2003

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Nextera XT Index Kit v2 Set D (96 indexes, 384 samples)

FC-131-2004

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Nextera XT Index Kit (24 indexes, 96 samples)

FC-131-1001

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Accessory Products
What accessories do I need?

Illumina Free Adapter Blocking Reagent (12 reactions)

20024144

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Illumina Free Adapter Blocking Reagent (48 reactions)

20024145

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Product Highlights

Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids
  • Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time
  • Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.
  • Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications
  • Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatible with all Illumina sequencers.

The IDT® for Illumina®-Nextera™ DNA UD Indexes Sets A, B, C, and D Indexes offer up to 384 unique dual indexing that enables accurate assignment of reads and efficient use of the flow cell.  These unique dual index codes use 10 base pair codes. This change in base pair index codes require adjustments to the sequencing run setup.

Find automation vendors with robotic systems compatible with this kit

This product is available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

When to Use Standard vs. Bead-Based Normalization

For high-throughput research, bead-based normalization can save time and resources by providing an accelerated path from DNA to data. This technical note provides guidelines and data comparing the two normalization methods to help you decide which option to use.

Read Tech Note
When to Use Standard vs. Bead-Based Normalization

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
MiSeq System Up to 384 samples per run Up to 2 x 300 bp
NextSeq 550 System Up to 384 samples per run Up to 2 x 150 bp

Product Comparison

Nextera XT DNA Library Preparation Kit Nextera DNA Flex Library Prep Kit
Assay Time ~5.5 hours from DNA extraction to normalized library. (Library prep time: ~90 minutes). ~3-4 hours (from DNA extraction to normalized library)
Description Fast library prep optimized for research on small genomes, PCR amplicons, and plasmids. A fast, flexible workflow for a wide range of research applications and sample types, from human to microbial whole-genome sequencing and more.
Hands-On Time 15 minutes 1-1.5 hours
Input Quantity 1 ng DNA Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. (For blood and saliva, see the reference guide).
Mechanism of Action Enzymatic fragmentation Bead-linked transposome
Method 16s rRNA Sequencing, Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing
Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together. Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations
Specialized Sample Types Low-Input Samples, Not FFPE-Compatible, Single Cells Blood, Not FFPE-Compatible, Saliva
Species Details Compatible with any species Compatible with any species
Target Insert Size 300 bp–1.5 kb ~500 bp

Method-Specific Workflow Example

 

Customer Stories

Mapping Neural Diversity: A Molecular Analysis of Neuronal Identity in Mouse Primary Visual Cortex

Single-cell mRNA-Seq with the MiSeq and HiSeq Systems is enabling Allen Institute researchers to classify V1 neural cells.

Read More
Next-Generation Sequencing Aids Researchers in the Fight Against the Ebola Virus

Genomic studies with the HiSeq and MiSeq Systems are enabling researchers to track Ebola outbreaks and understand the impact of the virus’s rapid mutation rate.

Read More

Supporting Data and Figures

 

Related Products

Nextera DNA Flex Library Prep Kit

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Nextera Mate Pair Library Prep Kit

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