ARTseq/TruSeq Ribo Profile

Powerful technique to study translation with sequencing

Ribosomal translation

  • Study active mRNA translation with sequencing
  • Sequence ribosome-protected mRNA
  • Predict protein abundance
  • Investigate translational control
  • Measure gene expression
  • Rapid, scalable spin-column method
  • No ultracentrifuge required!
  • Compatible with yeast and mammalian samples

Sequencing actively translated transcripts

Sequence mRNA fragments undergoing translation by ribosomes. These mRNA fragments are called "footprinted" or ribosome-protected mRNA fragments.

TruSeq Ribo Profile (formerly ARTseq*) sequences ribosome-protected mRNA fragments to provide a "snapshot" of the active ribosomes in a cell. You can identify proteins being actively translated from samples prepared with TruSeq Ribo Profile. Samples collected at different times often show changes in translation. Samples treated with different drugs often show different translation patterns.

Define open reading frames (ORFs) to enhance RNA-Seq information

ARTseq sequencing reads

Sequencing reads from ribosome-protected RNA define the open reading frame (ORF) for a representative yeast transcript (CWP2). TruSeq Ribo Profile RNA-Seq libraries prepared after size-exclusion chromatography (SEC) or sucrose gradient centrifugation (Sucrose) result in similar distributions of read densities. These read densities are enriched for ORFs and are devoid of untranslated regions (UTR).

TruSeq Ribo Profile results contain a higher percentage of coding RNA than Poly(A)+ samples

TruSeq Ribo Profile read percentages

Graphic representation of percent of reads that align to coding, UTR, intron, and intergenic regions of the transcript. The samples were prepared by size exclusion columns (SEC), sucrose gradient (Sucrose), or Poly(A)+. SEC and Sucrose-prepared samples contain more coding reads because the UTRs contain few ribosome-protected RNA fragments.

*The ARTseq library preparation kit has been renamed TruSeq Ribo Profile. Kits with either name on the label contain the same quality reagents and follow the same workflow.

Catalog IDs: RPYSC12116, RPHMR12126