TruSeq Exome Library Prep Kit

TruSeq Exome Library Prep Kit is a cost-effective library preparation and exome enrichment solution with industry-leading accuracy. It offers:

  • Pre-enrichment library pooling and optimal coverage for low-cost exome sequencing
  • Mechanical shearing and TruSeq enrichment technology that yield uniform coverage and greater than or equal to 80% on-target sequencing reads
  • Fully supported workflow solution to simplify exome sequencing

Cost-Effective Exome Sequencing

The TruSeq Exome Library Prep Kit supports 12-plex pre-enrichment library pooling, enabling researchers to maximize sequencing throughput and identify variants in less time by sequencing up to 12 libraries per flow cell lane.

The high on-target percentage requires fewer sequencing cycles to reach the optimal level of coverage, while still achieving high coverage uniformity for confident results. It also enables sequencing of more exomes per run, so researchers can maximize their budgets.


Percentage of On-Target Reads

Proven TruSeq Data Quality

The TruSeq Exome Library Prep Kit delivers ≥ 80% of on-target sequencing reads for efficient, cost-effective exome sequencing. By combining the TruSeq Exome Library Prep Kit with Illumina systems using sequencing by synthesis (SBS) technology, researchers can identify the highest number of true coding variants and minimize false-positive and false-negative calls.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Sequencing System No. of Exome Per Run at 50x No. of Exome Per Run at 100x
MiSeq Series 1 N/A
NextSeq Mid-output flow cell 3 2
NextSeq High-output flow cell 12 6
HiSeq 2500 Rapid-Run (dual flow cell) 24 12
HiSeq 2500 High-output (dual flow cell) 156 78
HiSeq 3000 96 48
HiSeq 4000 (dual flow cell) 192 96

Estimated number of exomes sequenced per run is calculated with a mean coverage of 50x (4 Gb) and 100x (8 Gb), respectively. Illumina recommends a 2 × 75 bp read length on all sequencers when using the TruSeq Exome Library Prep Kit.

Convenient, Integrated Workflow Solution

Illumina provides an integrated, fully supported workflow solution that guides researchers from library preparation through analysis. All components of the exome sequencing workflow are designed, optimized, and analytically validated together. Expert Illumina scientists provide a single source of technical and field support for every step in the process.


TruSeq Exome Library Prep Kit Configurations

Catalog Number TruSeq Exome Library Prep Kit Total Samples Number of Enrichment Reactions Number of Samples per Enrichment Reaction Unique index adapter combinations
FC-150-1001 8 rxn × 3 plex 24 8 3 3
FC-150-1002 8 rxn × 6 plex 48 8 6 6
FC-150-1003 8 rxn × 9 plex 72 8 9 9
FC-150-1004 8 rxn x 12 plex 96 8 12 96

Product Literature

Description Document Type File Info
TruSeq DNA Exome Data Sheet Data Sheet PDF< 1 MB
Evaluating DNA Quality from FFPE Samples Technical Note PDF< 1 MB

Catalog IDs: FC-150-1001, FC-150-1002, FC-150-1003, FC-150-1004