TruSeq Synthetic Long-Read DNA Library Prep Kit

The TruSeq Synthetic Long-Read DNA Library Prep Kit is a highly accurate, end-to-end solution that can be used for genome assembly or genome phasing. Depending on the analysis option you select, you can use it to:

  • Assemble synthetically long reads for de novo assembly and genome finishing applications
  • Perform genome phasing to identify co-inherited alleles and haplotype information, as well as phase de novo mutations

Accurately Construct Synthetic Long Reads

The TruSeq Synthetic Long-Read DNA Library Prep and Barcode Kits are designed for preparing DNA libraries to generate synthetically long reads. The library prep kit combines TruSeq and Nextera chemistries with synthetic long-read technology to prepare DNA libraries. The accompanying barcode kit includes 384 indexes for labeling the samples in each well. These indexes are then used after sequencing to construct synthetically long fragments for long-read assembly and phasing analysis.

Simplified Informatics for Genome Assembly or Whole Human Genome Phasing

After sequencing, push-button analysis in BaseSpace Sequence Hub simplifies assembly of long reads. Data can be transferred from an Illumina sequencing instrument to the BaseSpace Hub cloud instantly.

Genome assembly app: The TruSeq Long-Read Assembly App constructs long, synthetic reads from shorter sequencing reads for accurate genome assembly and genome finishing. TruSeq Synthetic Long-Read technology allows you to use the same familiar platform with a new application.

Phasing analysis app: The TruSeq Phasing Analysis App can be used to perform whole human genome phasing, identifying haplotype information and co-inherited alleles, and phasing de novo mutations. By constructing synthetically long fragments from shorter sequencing reads, this method provides more comprehensive and accurate phasing compared to conventional trio studies or statistical inference.

Library Preparation
Genomic DNA is fragmented to approximately 10 Kb.
Adapters are ligated to the fragments.
Fragments are clonally amplified across 384 wells. Fragments are sheared and labeled with unique indexes.
Fragments from all 384 wells are then pooled,
purified, and size selected.
Fragments are sequenced.

A BaseSpace App is used to assemble the long reads or phase a human genome

TruSeq Long-Read Assembly App Workflow

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TruSeq Phasing Analysis App Workflow

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Catalog IDs: FC-126-1001, FC-126-1002, FC-126-1003, FC-126-1004