TruSeq Targeted RNA Expression Kits offer highly customizable mid- to high-plex gene expression profiling and validation for desktop sequencers. Overcoming significant challenges in cost and workflow, TruSeq Targeted RNA assays deliver a fully integrated solution, including convenient online panel design and ordering, a rapid and simple workflow starting with as little as 50 ng of RNA, and automated, on-instrument data analysis.
User-defined panels of 12-1,000 assays can be created to target genes, isoforms, splice junctions, cSNPs, and fusion genes with the ability to multiplex up to 384 samples. Unleash the power of sequencing-based gene expression analysis, and generate data equivalent of up to 25,000 individual RT-PCR assays in a single run.*
For the ultimate in flexibility, custom content can be added to fully optimized, experimentally validated TruSeq Targeted RNA fixed panels focused on cell pathways and disease states.
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Dr. Hughes at University of Missouri-St. Louis uses TruSeq Targeted RNA Expression to uncover the genetic mechanisms of circadian rhythms.
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Human Brain Reference RNA (HBRR), Universal Human Reference RNA (UHRR), and human liver RNA samples were prepared using TruSeq Targeted RNA Expression Kits using a combination of the cell cycle and NFKB panels. These libraries were sequenced on the MiniSeq System using the new MiniSeq High Output Reagent Kit at a 1 x 51 bp read length configuration. The total yield was 2.04 Gb with 96.5% of bases at or above Q30.
For Research Use Only.
*Refers to instruments with catalog number SY-410-1001 and upgrade, or catalog number SY-410-1003
|Description||Document Type||File Info|
|Considerations for Designing a Successful TruSeq Targeted RNA Expression Experiment||Technical Note||PDF(< 1 MB)|
|TruSeq Targeted RNA Expression Data Sheet||Data Sheet||PDF(< 1 MB)|
|Expression Analysis of FFPE Samples||Technical Note||PDF(1 MB)|
Catalog IDs: RT-101-1001, RT-102-1001, RT-801-1001, RT-802-1001, RT-401-1001, RT-402-1001, RT-402-1002, RT-402-1003, RT-402-1004