TruSeq DNA PCR-Free Library Preparation Kit

TruSeq DNA PCR-Free Library Preparation Kits provide simple, all-inclusive library preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to whole-human genomes. The kits offer shortened gel-free workflows, the ability to sequence the most challenging regions, and the power to identify the greatest number of variants. Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

  • TruSeq DNA PCR-Free LT Library Preparation Kits for low-throughput studies. Includes 24 single indexes.
  • TruSeq DNA PCR-Free HT Library Preparation Kits for high-throughput studies. Includes 96 dual indexes pre-loaded in a 96-well plate.

Sequence the most challenging regions

TruSeq DNA PCR-free kits offer superior coverage of areas which are traditionally difficult to sequence such as high GC-rich regions, promoters, and repetitive content. The kits are tunable to a variety of read lengths and are supported on all of Illumina's sequencing instruments. This permits the researcher to tailor each run to the needs of the experiment.

Detect the greatest number of variants

PCR-free means reduced library bias and gaps. The result is unsurpassed data quality. This enables you to detect the greatest number of variants. Excellent genome coverage means your results have the lowest and smallest number of gaps and enhanced coverage of high GC/AT rich regions.

Use PCR-free for faster protocols

The industry's most widely adopted library preparation workflow for DNA sequencing is now enhanced. Removing PCR creates a faster protocol and superior data quality. Bead-based size selection shortens the workflow. In tandem with Illumina's sequencing systems, the TruSeq DNA PCR-Free Library Preparation Kit provides a range of enhancements to the industry's most widely adopted library preparation workflow.

Kits for limited available DNA and exome enrichment

TruSeq Nano DNA Library Prep is also available for efficient interrogation of samples with limited available DNA.

For enrichment, the Nextera Rapid Capture Exome and Expanded Exome assays provide a seamless end-to-end library preparation solution. These all-in-one library preparation and exome enrichment kits allow researchers to identify coding variants 70% faster than any other method. An expanded kit increases coverage to UTRs and miRNA.

Comparison of TruSeq DNA Library Preparation Kits

Specification TruSeq Nano DNA TruSeq DNA PCR-Free
Kit type For NeoPrep System LT and HT LT and HT
Description TruSeq Nano DNA workflow performed with the NeoPrep system and reagents Based upon widely adopted TruSeq library prep, with lower input and improved data quality Superior genomic coverage with radically reduced library bias and gaps
Workflow Simplified NeoPrep workflow with lower input and less hands-on time Manual workflow Manual workflow
Input quantity 25–75 ng 100–200 ng
1–2 μg
Includes PCR Yes Yes No
Assay time 7.5 hours (includes library prep through normalization) 5.5 hours ~5 hours
Hands-on time 30 minutes 4 hours ~4 hours
Target insert size 350 bp or 550 bp 350 bp or 550 bp 350 bp or 550 bp
Gel-Free Yes Yes Yes
Number of samples supported Up to 16 samples per NeoPrep library card 24 (LT) or 96 (HT) 24 (LT) or 96 (HT) samples
Size-selection beads Included Included Included
Applications Whole-genome sequencing applications, including whole-genome resequencing, de novo assembly, and metagenomics studies
Sample multiplexing 24 single indexes or 96 dual-index combinations
Compatible Illumina sequencers MiniSeq, MiSeq, NextSeq, HiSeq, HiSeq X, HiScanSQ, and Genome Analyzer systems


System Compatibility

Genome Analyzer IIx

Product Literature

Description Document Type File Info
Comparison of TruSeq Sample Preparation Kits Technical Note PDF< 1 MB
TruSeq DNA PCR-Free Data Sheet PDF8 MB

Catalog IDs: FC-121-3001, FC-121-3002, FC-121-3003