TruSeq Stranded Total RNA with Ribo-Zero Globin kits deliver the clearest, most complete view of the transcriptome from blood-derived RNA, with a fast, efficient sequencing library preparation workflow.
These kits couple the benefits of Ribo-Zero ribosomal RNA reduction chemistry with RNA sequencing (RNA-Seq) technology, providing a robust, scalable end-to-end solution for whole-transcriptome analysis of human, mouse, or rat samples. This technology provides:
Whole-transcriptome analysis of blood-derived RNA requires the removal of two forms of abundant RNA—ribosomal RNA (both cytoplasmic and mitochondrial) as well as globin mRNA, which is present in high levels in whole blood. Traditional removal methods require two independent steps. This means the need for additional reagents, a longer workflow, and more input RNA lost.
TruSeq Stranded Total RNA with Ribo-Zero Globin leverages Ribo-Zero chemistry to efficiently remove both forms of abundant RNA in a single, rapid step.
The kits are offered in low-throughput (LT) and high-throughput (HT) configurations (48 samples / 12 of 24 total indexes in Sets A and B, and 96 samples with 96 unique dual-index pairs, respectively). The TruSeq Stranded Total RNA LT Library Prep Kits A and B may be used together to pool up to 24 samples using the 12 different indexes in each kit. Protocols are available for preparing small or large numbers of samples.
Interested in stranded mRNA library prep? View the TruSeq Stranded mRNA Library Preparation Kit »
Get more information, protocols, and references on the RNA-Seq overview page »
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Catalog IDs: RS-122-2501, RS-122-2502, RS-122-2503