TruSeq Stranded Total RNA with Ribo-Zero Globin

TruSeq Stranded Total RNA with Ribo-Zero Globin kits deliver the clearest, most complete view of the transcriptome from blood-derived RNA, with a fast, efficient sequencing library preparation workflow.

High-quality whole-transcriptome analysis with precise strand information

These kits couple the benefits of Ribo-Zero ribosomal RNA reduction chemistry with RNA sequencing (RNA-Seq) technology, providing a robust, scalable end-to-end solution for whole-transcriptome analysis of human, mouse, or rat samples. This technology provides:

  • Uniform coverage and precise measurement of strand orientation
  • High-confidence discovery of features such as alternative transcripts, antisense expression, and allele-specific expression
  • Discovery across both coding and multiple forms of non-coding RNA

Efficient ribosomal RNA and globin mRNA removal in a single step

Whole-transcriptome analysis of blood-derived RNA requires the removal of two forms of abundant RNA—ribosomal RNA (both cytoplasmic and mitochondrial) as well as globin mRNA, which is present in high levels in whole blood. Traditional removal methods require two independent steps. This means the need for additional reagents, a longer workflow, and more input RNA lost.

TruSeq Stranded Total RNA with Ribo-Zero Globin leverages Ribo-Zero chemistry to efficiently remove both forms of abundant RNA in a single, rapid step.

Flexible kit configurations

The kits are offered in low-throughput (LT) and high-throughput (HT) configurations (48 samples / 12 of 24 total indexes in Sets A and B, and 96 samples with 96 unique dual-index pairs, respectively). The TruSeq Stranded Total RNA LT Library Prep Kits A and B may be used together to pool up to 24 samples using the 12 different indexes in each kit. Protocols are available for preparing small or large numbers of samples.

Related links

Interested in stranded mRNA library prep? View the TruSeq Stranded mRNA Library Preparation Kit »

Get more information, protocols, and references on the RNA-Seq overview page »

System Compatibility

Genome Analyzer IIx
Description Document Type File Info

Catalog IDs: RS-122-2501, RS-122-2502, RS-122-2503