TruSeq Custom Amplicon is a fully customizable, amplicon-based assay for targeted resequencing. The assay enables researchers to focus interrogation on key regions of genomic interest. Up to 1536 amplicons can be sequenced in a single reaction using a simple workflow. This highly targeted approach offers unparalled efficiency for discovering, validating, and screening genetic variants in areas of research focus.
Requiring as little as 50 ng of input genomic DNA (gDNA), this kit can be used with a wide range of sample types. The ability to multiplex up to 1536 amplicons per reaction allows coverage of up to 650 kb of cumulative sequence. Further, an integrated dual indexing scheme supports up to 96 samples per run, enabling researchers to fully benefit from the desktop sequencer output capabilities.
This assay provides improved performance in difficult to address GC-rich regions, as well as increased library yield, uniformity, and stability. Additionally, optimized amplification steps enable sample input requirements to drop from 150 ng to 50 ng.
The kit offers a fully integrated DNA-to-data solution, including convenient probe design and ordering using DesignStudio, an easy-to-use online software tool that provides dynamic feedback to optimize design and region coverage. Automated data analysis using the TruSeq Amplicon BaseSpace App allows researchers to easily review project data, including variants detected, across multiple runs.
Dr. Daber uses TruSeq Custom Amplicon for clinical research to better understand cancer prognosis and therapeutic choices.
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The TruSeq Custom Amplicon Assay is a simple and streamlined method for capturing and amplifying targeted regions of interest.
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Find out more about TruSeq Custom Amplicon (TSCA). See how you can target hundreds of genes with simple, customized design. Detect rare somatic mutations below 5%. Prepare and sequence ~150,000 amplicons on the MiSeq System in less than two days, with automated analysis.
Two amplicon-based options for targeted sequencing are now available: TruSeq Custom Amplicon Low Input for low-input DNA and FFPE, or TruSeq Custom Amplicon 1.5 for very high plexity pools or longer amplicons.
TruSeq Custom Amplicon Low Input enables you to detect and discover high-quality information, even from limited and challenging samples. Use the table to compare assays.
|Technology||TruSeq Custom Amplicon Low Input||TruSeq Custom Amplicon v1.5|
|Amplicon plexity (single pool)||Up to 1536||Up to 10,000 (via Concierge)|
|Input requirement||as low as 10 ng||50 ng|
|FFPE Compatibility||Yes – 10 ng (FFPE DNA input dependent on quality)||Yes – 250 ng|
|Amplicon size||150, 175, 250 bp||150, 175, 250, 425 bp|
|Instrument||MiniSeq, MiSeq, NextSeq||MiniSeq, MiSeq, NextSeq|
|Read Length||Up to 2 x 150 bp||Up to 2 x 300 bp|
|Time||6.5 hours||10 hours|
|Kit size (samples)||16, 96+||96+|
|Description||Document Type||File Info|
|TruSeq Custom Amplicon v1.5||Data Sheet||PDF(< 1 MB)|
Catalog IDs: FC-130-1001, FC-130-1003, FC-130-1006, FC-130-1007