NextSeq 500/550 v2 Kits

NextSeq 500/550 v2 sequencing reagent kits bring the power of a high-throughput sequencing system to the desktop, now with improved data quality and the highest yield of error-free reads. These kits offer:

  • Robust base calling and improved signal to noise ratio
  • Multiple options for both sequencing output and read length
  • Simplified reagent cartridge configurations, with improved workflow and cartridge loading
  • Intuitive labeling and RFID-encoded reagents
  • Optimized chemistry with the latest in SBS and bridge-amplification cluster generation

Configurations for every application
Researchers have a wide range of solutions for different applications from ChIP-Seq and RNA profiling to whole-genome sequencing, as shown in the table. NextSeq reagent kits provide industry leading accuracy with paired-end sequencing capability built into every reagent kit.

NextSeq 500/550 Kits High Output†
up to 400M Clusters
Mid Output†
up to 130M Clusters

300 cycles
  • Whole Genome
  • de novo
  • Exome
  • Targeted Panels
  • Exome

150 cycles
  • Whole Transcriptome
  • mRNA-Seq
  • Exome
  • Whole Transcriptome
  • mRNA-Seq
  • Exome

75 cycles


  • Gene Expression Profiling
  • NIPT

†All NextSeq 500/550 kits are paired-end capable

Ready-to-use cartridges redefine ease-of-use
Pre-configured reagent kits for the NextSeq Series Systems provide all the components needed for sequencing on the platform in three ready-to-use cartridges (reagent, flow cell, and buffer). Simply load user-prepared library pools directly into the NextSeq reagent cartridge; the system automatically performs all steps necessary for template amplification and sequencing without user intervention. NextSeq 500/550 v2 sequencing reagent kits provide simplified cartridge loading with the bleach and dual-indexing primers already in the cartridge.

The kits deliver powerful sequencing chemistries with as little as 10 minutes of hands-on time. Intuitive labeling and RFID-encoded reagents ensure compatibility with user-defined run configurations.

Catalog IDs: FC-404-2001, FC-404-2002, FC-404-2003, FC-404-2004, FC-404-2005

The NextSeq 500/550 v1 kits have been discontinued and replaced by the NextSeq 500/550 v2 kits. Please contact your local Account Manager if you need assistance in transitioning to the new NextSeq 500/550 v2 kits.

Visit the NextSeq 500/550 Kits Support page for more information.