The Nextera Mate Pair Sample Preparation Kit offers the only gel-free method for preparing up to 12 kb mate pair libraries with the industry’s lowest DNA input requirement. It is an ideal approach for de novo sequencing, genome finishing, and the detection of structural variation — even in the most complex genomes such as those derived from cancer.
Both gel-free and gel-plus protocols are available. The gel-free protocol allows mate pair sequencing when limited DNA is available:
The gel-plus protocol is ideal for challenging mate pair applications, such as de novo assembly of complex genomes and detection of structural variation.
Inclusion of the TruSeq DNA Sample Preparation master-mixed reagents in the Nextera Mate Pair kit reduces the number of assay steps, thereby reducing the hands-on time required.
The Nextera Mate Pair Sample Preparation Kit provides identifiable junction sequences for accurate identification of the two halves of the mate pair fragment.
“Nextera Mate Pair and the HiSeq System, make it so easy to assemble genomes and come to biological conclusions based on the data.”
— Researcher at the Max Planck Institute for Evolutionary Anthropology
|Description||Document Type||File Info|
|Nextera Mate Pair Library Preparation Kit||Data Sheet||PDF(< 1 MB)|
|De Novo Assembly of Small Genome Nextera Mate Pair Libraries with a Single MiSeq System Run||Application Note||PDF(< 1 MB)|
|Data Processing of Nextera Mate Pair Reads on Illumina Sequencing Platforms||Technical Note||PDF(1 MB)|
|De Novo Assembly of Bacterial Genomes||Application Note||PDF(< 1 MB)|
Catalog IDs: FC-132-1001