Products

SurePlex DNA Amplification System

Used to generate a DNA template from single cells. Ideal for use with PGS research applications.

~3 hours

Assay time

~1 hr

Hands-on time

Single cell or multi-cell samples

Input quantity

See full details in the specifications table

Overview

Whole-genome amplification technology can be used to increase the quantity of DNA in a single cell or a few cells. This amplification ensures sufficient amounts of DNA for next-generation sequencing of preimplantation genetic screening (PGS) samples.

The SurePlex DNA Amplification System reproducibly amplifies total DNA from single cells or a few cells of an embryo biopsy. It follows an easy-to-use single tube protocol to produce 2-5 μg of amplified DNA in 2 hours. Following SurePlex DNA amplification, samples are ready for use in research applications such as VeriSeq PGS (available from Vitrolife).

Specifications

Assay time ~3 hours
Hands-on time ~1 hr
Input quantity Single cell or multi-cell samples
Method Targeted DNA Sequencing
Nucleic acid type DNA
Specialized sample types Single Cells
Species category Human
Technology Sequencing
Variant class Chromosomal abnormalities, Structural variants, Copy number variants (CNVs)

Applications

Related applications and methods

Preimplantation genetic screening research

Preimplantation genetic screening (PGS), also known as PGT-A, determines the chromosomal status of an embryo by screening all 23 chromosome pairs.

Karyomapping research

Karyomapping techniques can assess the likelihood of an embryo carrying a gene mutation that may be linked to an inherited genetic disorder.

Cytogenomics

Together, next-generation sequencing and arrays enable cytogenomic analysis of chromosome aberrations for constitutional and cancer research.

Documentation

Product literature

Literature is not currently available for this product.

Support documentation

SurePlex DNA Amplification System (50 reactions)

PR-40-415101-00

Buffers and reagents for 50 reactions to amplify total DNA from single cells. Intended for use with 24sure assay or VeriSeq PGS applications.

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