Ordering the NextSeq 1000 and NextSeq 2000 Sequencing Systems

Order a NextSeq 1000 or NextSeq 2000 System today. Speak to a representative for information on pricing, trade-in options, and more.

XLEAP-SBS reagents for P1, P2, and P3 flow cells available Q2 of 2024.

In 1H 2024, P4 shelf life will be 3 months. This will be extended to 6 months in 2H of 2024.

Documentation

Product literature

Required products

Required products:

Optional products:

  • NextSeq 1000/2000 Read and Index Primers
  • PhiX Control v3 for use as a sequencing control
  • Air filter replacement
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Resources

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How do I prepare for my Illumina instrument delivery?

An Illumina Field Applications Scientist discusses what to expect during the preinstallation, shipping, and delivery stages. We’ll highlight important steps in the process and introduce you to available resources.

Get up and running quickly with a rental or leasing program designed to work with your budget.

Learn why researchers choose the NextSeq 1000 and NextSeq 2000 Systems for efficient, versatile, and easy-to-use sequencing.

Identify the best approach, manage your workflow, purchase what you need, and get support.  

Instrument (3)

NextSeq 2000 Sequencing System

20038897

Illumina NextSeq 2000 Sequencing System is an integrated system for automated generation of DNA clonal clusters by bridge amplification, sequencing, primary analysis, and secondary analysis. System includes embedded touchscreen monitor and on-instrument computer, control software, hardware accelerated Dragen Bio-IT secondary analysis pipelines, installation and training, and 12 months warranty (including parts and labor).

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NextSeq 1000 Sequencing System

20038898

Illumina NextSeq 1000 Sequencing System is an integrated system for automated generation of DNA clonal clusters by bridge amplification, sequencing, primary analysis, and secondary analysis. System includes embedded touchscreen monitor and on-instrument computer, control software, hardware accelerated Dragen Bio-IT secondary analysis pipelines, Installation and training, and 12 months warranty (including parts and labor).

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NextSeq 1000 to NextSeq 2000 Upgrade

20047256

The NextSeq 1000 upgrade option enables users to seamlessly upgrade their current NextSeq 1000 instrument to enjoy the benefits of the NextSeq 2000. This includes the ability to run all flow cells currently supported on the NextSeq 2000, including higher output products such as the P3 flow cell.

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XLEAP-SBS reagents (7)

NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (300 Cycles)

20100992

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).

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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (200 Cycles)

20100993

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).

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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (100 Cycles)

20100994

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).

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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (50 Cycles)

20100995

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 50 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).

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NextSeq™ 1000/2000 XLEAP-SBS™ Read and Index Primer Kit

20112856

Reagents to utilize custom read and index primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge. This kit is sufficient for 1 custom read primer and 1 custom index primer use.

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NextSeq™ 1000/2000 XLEAP-SBS™ Index Primer Kit

20112858

Reagents to utilize custom index primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge. This kit is sufficient for 10 custom index primer uses.

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NextSeq™ 1000/2000 XLEAP-SBS™ Read Primer Kit

20112859

Reagents to utilize custom read primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge. This kit is sufficient for 10 custom read primer uses.

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Standard SBS reagents (10)

NextSeq 1000/2000 P1 Reagents (300 Cycles)

20050264

Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support (up to 100M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (300 cycles), NextSeq 1000/2000 P1 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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NextSeq™1000/2000 P1 Reagents(600cycles)

20075294

Provides kitted reagents for 600 cycles of sequencing, plus dual- indexing support (up to 100M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (600 cycles), NextSeq 1000/2000 P1 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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NextSeq 1000/2000 P2 Reagents (100 Cycles) v3

20046811

Provides kitted reagents for 100 cycles of sequencing, plus dual- indexing support (up to 400M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (100 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20.

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NextSeq 1000/2000 P2 Reagents (200 Cycles) v3

20046812

Provides kitted reagents for 200 cycles of sequencing, plus dual- indexing support (up to 400M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (200 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20.

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NextSeq 1000/2000 P2 Reagents (300 Cycles) v3

20046813

Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support (up to 400M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (300 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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NextSeq™1000/2000 P2 300M Reagents(600cycles)

20075295

Provides kitted reagents for 600 cycles of sequencing, plus dual- indexing support (up to 300M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (600 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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NextSeq 2000 P3 Reagents (50 Cycles)

20046810

Provides kitted reagents for 50 cycles of sequencing, plus dual-indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (50 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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NextSeq 2000 P3 Reagents (100 Cycles) 

20040559

Provides kitted reagents for 100 cycles of sequencing, plus dual- indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (100 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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NextSeq 2000 P3 Reagents (200 Cycles)

20040560

Provides kitted reagents for 200 cycles of sequencing, plus dual- indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (200 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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NextSeq 2000 P3 Reagents (300 Cycles)

20040561

Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (300 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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Accessory products (5)

NextSeq 1000/2000 Read and Index Primers

20046115

Reagents to utilize custom read and index primers with the NextSeq 1000/2000 cartridge. This kit is sufficient for 1 custom read primer and 1 custom index primer use.

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NextSeq 1000/2000 Index Primer Kit

20046116

Reagents to utilize custom index primers with the NextSeq 1000/2000 cartridge. This kit is sufficient for 10 custom index primer uses.

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NextSeq 1000/2000 Read Primer Kit

20046117

Reagents to utilize custom read primers with the NextSeq 1000/2000 cartridge. This kit is sufficient for 10 custom read primer uses.

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PhiX Control v3

FC-110-3001

Kitted DNA control for the Illumina sequencing platform. Compatible with Single and Paired End reads up to 150 base pairs. (10ul of 10nM template solution)

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NextSeq 2000/1000 Single FILTER, IM

20029759

NextSeq 2000/1000 Single FILTER, IM

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FAQs

The NextSeq 1000 System is a lower cost instrument that runs P1 and P2 flow cells, offering fast turnaround times and lower throughput. The NextSeq 2000 System accesses a wider range of throughputs with P1, P2, P3, and P4 flow cells for more sequencing per sample, larger studies, and better run economics.

XLEAP-SBS chemistry is available on NextSeq 2000 with the new P4 flow cell. XLEAP-SBS chemistry will be available on P1, P2, and P3 flow cells in Q2 2024*.

*P3 and P4 are only available on the NextSeq 2000 System

 

Yes, both systems use patterned flow cells to offer high cluster density, reducing the run cost. In these flow cells, monitoring the Cluster PF (%) is a better measure of potential cluster occupancy than measuring cluster density.

Yes, Illumina offers the option to trade in your existing NextSeq 1000 System for a NextSeq 2000 System. Restrictions apply. To learn more about this option and eligibility, contact a sales representative.

NextSeq 1000 and NextSeq 2000 System analysis options include:

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Trade in your HiSeq, MiSeq, or NextSeq 500/550 system

If you currently own a HiSeq, NextSeq 500/550, or a MiSeq System, you may be eligible to trade in your existing instrument for a NextSeq 1000 or NextSeq 2000 System. Restrictions apply. Contact a sales representative using the form below to learn more about eligibility.

Order the NovaSeq X Series

Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.

MiSeq Reagent Kit v3

Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.

TruSight Oncology 500

Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

Speak with a specialist

Reach out for information about our products and services, or get answers to questions about our technology.