You need technology that keeps pace with your goals. When you partner with Illumina, you're backed by a globally trusted provider and years of expertise, so you can spend less time troubleshooting and more time driving meaningful progress. Purpose-built for versatility and enduring performance, the NextSeq 1000 and NextSeq 2000 Systems streamline workflows, enable flexibility across applications, and deliver consistent results in even the most demanding environments.
Deliver reliable insights with the NextSeq 1000 and NextSeq 2000 Systems
Built for your next breakthrough
Proven performance trusted by labs worldwide
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Maximize your productivity with scalable sequencing
Deliver trusted results with robust technology
Get a head start with trusted protocols, global expertise, and a wealth of benchmarking data. Whether you're scaling up, validating new workflows, or aligning with industry standards, you're building on a platform that’s already delivering results around the world.
Deliver trusted results with robust technology
Get a head start with trusted protocols, global expertise, and a wealth of benchmarking data. Whether you're scaling up, validating new workflows, or aligning with industry standards, you're building on a platform that’s already delivering results around the world.
Single-cell RNA sequencing
Illumina Single Cell 3' RNA Prep is an accessible and scalable solution for high-performance single-cell RNA-Seq. The wide sequencing range of the assay, from hundreds to hundreds of thousands of cells, on NextSeq 1000 and NextSeq 2000 Systems supports research needs from low cell–diversity pilot studies to complex tissue analysis. This flexible solution eliminates the need for specialized instrumentation or microfluidics, making single-cell RNA-Seq accessible for more labs.
Oncology sequencing
TruSight Oncology 500 v2 and TruSight Oncology 500 ctDNA v2 deliver fast, in-house comprehensive genomic profiling (CGP) from tissue and liquid biopsy samples with results in 3-4 days. Built on powerful Illumina SBS chemistry and data analysis tools, these solutions streamline CGP research on NextSeq 1000 and NextSeq 2000 Systems.
Future-proof your workflows with a flexible benchtop instrument
Learn how NextSeq 1000 and NextSeq 2000 Systems enable cutting-edge applications from whole-exome sequencing to single-cell transcriptomics to metagenomics—right on your benchtop. The NextSeq 1000 and NextSeq 2000 Systems are designed to grow with you, so you can confidently pivot as your studies evolve.
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The era of AI-powered gene therapy research is here
Watch how Dyno Therapeutics is solving the complex puzzle of in vivo gene delivery by combining protein engineering, AI, and next-generation sequencing to create smarter, scalable solutions for gene therapy, all powered by the NextSeq 2000 System.
Make the next leap possible with NextSeq 2000
Stay ahead with a platform that evolves
NextSeq 1000 and 2000 Systems are built for today’s research and the needs for tomorrow. With adaptive architecture designed to support new assays and protocols, you can future-proof your workflows with confidence. Backed by the industry leader in sequencing, the NextSeq 1000 and 2000 Systems ensure that you're always ready to take advantage of the latest assays, technologies, and applications.
Coming soon in 2026
- Longer reads with a 2 × 500 kit
- Higher data quality with a Q40 score
- Increased flow cell use to run more low-diversity samples
- Enhanced user traceability with new account access and monitoring
Proven innovations
2020: Launched the NextSeq 1000 and NextSeq 2000 Systems, now the market-leading mid-throughput sequencing systems.
2021: Unlocked targeted panels with new kit options.
2022: Enabled metagenomics and immune repertoire profiling with longer read kits.
2024: Released XLEAP-SBS chemistry, enabling faster, cheaper, higher-quality sequencing. Developed the in-house CGP using TruSight Oncology 500 solution.
2025: Simplified single-cell sequencing with our proprietary Illumina Single Cell Prep.
Explore flexible financing plans designed for you
Get the sequencing power that you need, on your terms. From scaling your studies to expanding into new applications, flexible financing options make it easier to bring the NextSeq 1000 and 2000 Systems into your workflow. Choose from tailored leasing, reagent rental, or purchasing plans so you can grow with your needs for tomorrow.
Speak to a specialist about choosing Illumina for your benchtop sequencer
Get in touch to find out how Illumina benchtop sequencing can support your research efforts.
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