NovaSeq 6000 Sequencing System

Drive your discoveries here

A robust and scalable system designed to adapt to your needs and bring discoveries within reach

NovaSeq 6000 Sequencing System

The NovaSeq 6000 System: Shaping the future of genomics

Since its launch, the NovaSeq 6000 Sequencing System has revolutionized genomics studies. Hear how the system has continued to evolve to meet customer demands and shape what’s possible in the future of genomics.

Scientist with NovaSeq 6000 in laboratory
Scalable throughput for dynamic study sizes

So many ways to sequence. Scale up and down with a tunable output of up to 6 Tb and 20B single reads in < 2 days.

NovaSeq 6000 reagents
Broad, deep sequencing for clear answers

Accelerate your science with reliable sequencing breadth and depth for every project.

NovaSeq 6000 flow cells
Tunable output for mix and match options

Do what you want. Mix and match flow cell types and run one or two flow cells at a time. Choose between multiple read lengths, workflows, and more.

Tried and true performance

NovaSeq 6000 instrument specifications

System specifications

  • Output range
    80–6000 Gb
  • Single reads per run
  • Max read length
    2 × 250 bp
  • Run time
    13–44 hr

A flexible workflow designed to help you accelerate your research and maximize results

Manage workflow

Manage your workflow seamlessly with Clarity LIMS software or your existing LIMS system.

Prepare libraries

Prepare libraries with a broad range of high-performance library preparation kits.


Generate sequences with ready-to-use reagents featuring proven Illumina sequencing by synthesis chemistry.

Analyze data

Leverage Illumina DRAGEN software or BaseSpace Sequence Hub for accurate, rapid secondary analysis.

Interpret results

Gain insights and drive discovery with variant analysis and interpretation software.

Advanced applications. Impactful discoveries.

The NovaSeq 6000 System offers deep, broad coverage through advanced applications for a comprehensive view of the genome. Unlock a full spectrum of genetic variation and biological function with high-throughput sequencing.

NovaSeq 6000 in lab

Compare with NovaSeq X Series


NovaSeq 6000 System

NovaSeq X System

NovaSeq X Plus System

Run time 13–44 hr 13–48 hr 13–48 hr
Maximum output per run 6 Tb 8 Tb 16 Tb
Maximum reads per run

20B single reads

40B paired-end reads

26B single reads

52B paired-end reads

52B single reads

104B paired-end reads

Maximum read length 2 × 250 bpa 2 × 150 bp 2 × 150 bp
DRAGEN data analysis offerings

On-premises or cloud

DRAGEN Germline

DRAGEN Enrichment

DRAGEN Somatic


DRAGEN Illumina Complete Long Read WGS


DRAGEN Germline

DRAGEN Enrichment


DRAGEN Illumina Complete Long Read WGS


DRAGEN Germline

DRAGEN Enrichment


DRAGEN Illumina Complete Long Read WGS

a. Specification based on dual flow cell run of SP flow cells.

Popular products

NovaSeq 6000 Reagent Kits

Reagent kits for the NovaSeq 6000 System consisting of ready-to-use pre-filled reagent cartridges and flow cells.

NovaSeq 6000 Xp Workflow

NovaSeq 6000 Xp Workflow delivers the flexibility of independent lane loading and high library multiplexing per flow cell, maximizing sample throughput on a single run.

Illumina RNA Prep with Enrichment

A fast, integrated workflow for producing enriched and indexed sequencing libraries from a broad range of sample types and RNA input quantities.

Illumina DNA PCR-Free Prep

An efficient, fast, and integrated library preparation workflow that yields highly accurate data for sensitive sequencing applications.

Speak with a specialist

Get help deciding if the flexibility and throughput of the NovaSeq 6000 System are right for you. Contact us today for more information.