Sequencing
See What Sequencing Can Do For You
Sequencing may be utilized to determine the order of nucleotides in small targeted genomic regions or entire genomes. Illumina sequencing enables a wide variety of applications, allowing researchers to ask virtually any question related to the genome, transcriptome, or epigenome of any organism. Next-generation sequencing (NGS) methods differ primarily by how the DNA or RNA samples are prepared and the data analysis options used.
Sequencing Instrument Portfolio
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Key Sequencing Methods
DNA Sequencing
Analyze the entire genome, focus on regions of interest with whole-exome and targeted sequencing, or study DNA-protein interactions.
RNA Sequencing
Take advantage of a broad range of techniques, from targeted RNA to single-cell and whole-transcriptome sequencing.
Methylation Sequencing
Both genome-wide analysis and targeted approaches can provide insight into methylation patterns at a single nucleotide level.
High-Throughput Sequencing
Cost-effectively run data-rich applications and process more samples to increase statistical power.
Long-Read Sequencing
Long-read sequencing is a DNA sequencing technique that enables the sequencing of much longer DNA fragments than traditional short-read sequencing methods.
Library Preparation
Find out more about how library prep works, and explore user-friendly solutions. Options are available for a broad range of sequencing methods, including whole-genome sequencing, whole-exome and targeted sequencing, RNA sequencing, methylation sequencing, and more.
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Common Sequencing Applications
Cancer Research
NGS-based sequencing methods allow cancer researchers to detect rare somatic variants, tumor subclones, and circulating DNA fragments. Explore cancer sequencing methods.
Microbiology Research
From environmental metagenomics studies to infectious disease surveillance and more, sequencing can help researchers gain genetic insight into bacteria and viruses. Learn more about microbial genomics.
Complex Disease Research
Illumina sequencing is introducing new avenues for understanding autoimmune and rheumatic diseases, atherosclerosis, neurological diseases, and psychiatric disorders on a molecular level. Learn more about complex disease genomics.
Reproductive Health
Illumina sequencing and array technologies deliver fast, accurate information that can guide choices along the reproductive health journey. Explore reproductive health solutions.
Beginner's Guide to Next-Generation Sequencing
Considering bringing next-generation sequencing to your lab, but unsure where to start? These resources cover key topics in NGS and are designed to help you plan your first experiment.
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Additional Tips
Explore Sequencing Troubleshooting Tips
These videos provide expert tips for common issues such as overclustering, inconsistent quantitation, preventing contamination, and determining if your sequencing insert is too short.
Find Content and Products for Your Field
The user-friendly "Recommended Links" feature allows you to easily find content and products relevant to your specific field of interest. You can access this option from the top of any illumina.com page.
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Additional Resources
Introduction to NGS
NGS has revolutionized the biological sciences. Learn how it compares to conventional methods, and find out how Illumina technology works.
HIV-1 Tropism Studies
Deep sequencing enables researchers to assess HIV Type 1 coreceptor usage.
COVID-19 Solutions
NGS is uniquely positioned in an infectious disease surveillance and outbreak model. Explore methods and find solutions to detect and characterize SARS-CoV-2 and other respiratory pathogens, track transmission routes, study co-infection, and investigate viral evolution.