TruSight Oncology 500 ctDNA v2

Enable CGP with a pan-cancer panel capable of detecting key IO gene signatures (TMB, MSI) plus all main variant classes from ctDNA in blood plasma. Read More...

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Manual Prep

TruSight Oncology 500 ctDNA v2 (24 samples)

20105899

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TruSight Oncology 500 ctDNA v2 for Use with NovaSeq 6000 S2 Flow Cell (24 samples)

20105901

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TruSight Oncology 500 ctDNA v2 for Use with NovaSeq 6000 S4 Flow Cell (24 samples)

20105902

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TruSight Oncology 500 ctDNA v2 (24 samples) plus Velsera Interpretation Report

20105905

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TruSight Oncology 500 ctDNA v2 plus Velsera Interpretation Report, for Use with NovaSeq 6000 S2 Flow Cell (24 samples)

20105907

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TruSight Oncology 500 ctDNA v2 plus Velsera Interpretation Report, for Use with NovaSeq 6000 S4 Flow Cell (24 samples)

20105908

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TruSight Oncology 500 ctDNA Kit (48 samples)

20039252

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TruSight Oncology 500 ctDNA Kit plus Velsera Interpretation Report (16 indexes, 48 Samples) 

20043410

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TruSight Oncology 500 ctDNA Kit for use with NovaSeq 6000 S2

20101995

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TruSight Oncology 500 ctDNA Kit for use with NovaSeq 6000 S4

20101998

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TruSight Oncology 500 ctDNA Kit for use with NovaSeq 6000 S2 plus Velsera interpretation report (16 indexes, 48 samples)

20102000

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TruSight Oncology 500 ctDNA Kit for use with NovaSeq 6000 S4 plus Velsera interpretation report (16 indexes, 48 samples)

20102001

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Automation

TruSight Oncology 500 ctDNA v2 for Automation (48 samples)

20105900

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TruSight Oncology 500 ctDNA v2 Automation Kit, for Use with NovaSeq 6000 S2 Flow Cell (48 samples)

20105903

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TruSight Oncology 500 ctDNA v2 Automation Kit, for Use with NovaSeq 6000 S4 Flow Cell (48 samples)

20105904

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TruSight Oncology 500 ctDNA v2 for Automation (48 samples) plus Velsera Interpretation Report

20105906

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TruSight Oncology 500 ctDNA v2 Automation Kit plus Velsera Interpretation Report, for Use with NovaSeq 6000 S2 Flow Cell (48 samples)

20105909

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TruSight Oncology 500 ctDNA v2 Automation Kit plus Velsera Interpretation Report, for Use with NovaSeq 6000 S4 Flow Cell (48 samples)

20105910

Price
 
 

Index Adapters

IDT® for Illumina® UMI DNA/RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples)

20034701

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IDT® for Illumina® UMI DNA/RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples)

20034702

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IDT for Illumina UMI DNA/DNA Index Anchors Set A for Automation

20066404

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IDT for Illumina UMI DNA/DNA Index Anchors Set B for Automation

20063213

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Reagents

NovaSeq 6000 S2 Reagent Kit v1.5 (300 cycles)

20028314

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NovaSeq 6000 S4 Reagent Kit v1.5 (300 cycles)

20028312

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Software and Informatics Options

Illumina DRAGEN Server v4

20051343

Price
 
 

ICA Basic Annual Subscription

20044874

ICA Professional Annual Subscription

20044876

ICA Enterprise Annual Subscription

20038994

ICA Enterprise Service and Compliance Add-on (applies to Basic only)

20066830

Illumina Analytics - 1 iCredit

20042038

Price
 
 

Illumina Analytics  Starter Pack - 1,000 iCredits

20042039

Price
 
 

Illumina Analytics  - 5,000 iCredits

20042040

Price
 
 

Illumina Analytics  - 50,000 iCredits

20042041

Price
 
 

Illumina Analytics - 100,000 iCredits

20042042

Price
 
 

Illumina Connected Insights-Annual Subscription

20090137

Illumina Connected Insights - Research - Annual Subscription

20112516

Illumina Connected Insights‒Oncology Genome Equivalent Sample-VCF

20090138

Illumina Connected Insights Training - Remote

20092376

Informatics Professional Services

20071787

Price
 
 

Product Highlights

TruSight Oncology 500 ctDNA v2 is a pan-cancer NGS assay that enables in-house comprehensive genomic profiling (CGP) of circulating tumor DNA (ctDNA) in blood plasma for research.

  • Leverage minimally invasive blood samples as a complement or alternative to tissue biopsy
  • Target 523 genes to assess DNA variants across major variant classes (SNV, MNV, indels, CNV, and gene rearrangements), plus key immuno-oncology gene signatures (TMB, MSI)
  • Achieve ultra-sensitive detection from minimal cfDNA input
  • Get final reports in <4 days from cfDNA with a streamlined workflow

Low-level biomarker detection

Achieve sensitive and accurate detection (0.2% VAF for SNVs) with low inputs (20 ng) using UMI-based hybrid-capture library prep and deep sequencing.

Integrated bioinformatics

DRAGEN secondary analysis powers a rapid variant calling algorithm on-premise or in the cloud with Illumina Connected Analytics.

  • Data interpretation is streamlined using integrated reporting with the Illumina Connected Insights* product line or Velsera Clinical Genomics Workspace.
  • Achieve a fully automated analysis workflow with sequencer integration and autolaunch capabilities of Connected Analytics.

Streamlined workflow

Advanced chemistry and extensive workflow optimization enable a single-day library prep plus enrichment for a total turnaround time of <4 days. Automation-friendly kits and methods enable increased efficiencies.

*Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.

TruSight Oncology ctDNA 500 v2

Illumina improved the analysis of circulating tumors with TruSight Oncology ctDNA 500 v2. Learn about new features and benefits in the video.

Enable CGP with a comprehensive portfolio

Maximize chances of identifying an actionable alteration with the TruSight Oncology 500 product line.

TruSight Oncology 500 ctDNA v2

Analyze circulating tumor DNA (ctDNA) in blood plasma with similar DNA panel content as TruSight Oncology 500.

TruSight Oncology 500

Assess relevant DNA and RNA cancer biomarkers from FFPE tumor tissue, now including HRD through an accessory kit to assess the Genomic Instability Score (GIS).

TruSight Oncology 500 High-Throughput Assay

Batch up to 192 samples at a time while using the same panel content and tissue input type as TruSight Oncology 500.

HRD is only available with the addition of the TruSight Oncology 500 HRD kit to the TruSight Oncology 500 kit. Not available in Japan.

Learn more about the TruSight Oncology 500 product line

brochure Illumina TSO 500 Portfolio cover

TruSight Oncology 500 portfolio brochure

Interested in learning more about the TruSight Oncology 500 portfolio of products?

Download the overview brochure

Have questions about our products? Connect with an Illumina representative.

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NovaSeq 6000 System 24 samples per run (S4 flow cell), 800M paired-end reads, 35,000x coverage 2 × 150 bp
NovaSeq 6000 System 8 samples per run (S2 flow cell), 800M paired-end reads, 35,000x coverage 2 × 150 bp

Product Comparison

TruSight Oncology 500 ctDNA v2 TruSight Oncology 500 TruSight Oncology 500 High-Throughput
Cancer Type Pan-Cancer, Solid Tumor Pan-Cancer Pan-Cancer
Content Specifications Targeted selection of 523 genes (full coding sequence) for a total of 1.94Mb panel size.
• Immuno-oncology Biomarker Coverage: TMB and MSI
• Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types
• Clinical Trials Coverage: Over 600 clinical trials*
Targeted selection of DNA from 523 genes of interest, and RNA from 55 genes, for a total of 1.94Mb panel size.
• Immuno-oncology Biomarker Coverage: TMB and MSI
• Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types
• Clinical Trials Coverage: Over 600 clinical trials*
Hands-On Time ~2.5 hours for library prep and enrichment Manual: ~10.5 hrs
Automated: ~2.5 hrs (TruSight Oncology 500 only), Manual: ~10.5 hrs
Automated: ~2.5 hrs (TruSight Oncology 500 only)
Manual: ~10.5 hrs
Automated: ~2.5 hrs
Input Quantity 20 ng cfDNA (4 ml of plasma) 40 ng DNA, 40 ng RNA 40 ng DNA, 40-80 ng RNA (need at least 2 mm3 FFPE tissue)
Method Target Enrichment, Target Enrichment, Targeted DNA Sequencing Target Enrichment, Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing Target Enrichment, Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing
Multiplexing Up to 8 on S2, and 24 on S4 using Xp-4 Lane workflow, 16 indexes maximum Up to 8-plex 192 IDT for Illumina Nextera DNA UD Indexes (used for TSO500 HT DNA and RNA libraries)
Nucleic Acid Type DNA RNA, DNA RNA, DNA
Specialized Sample Types Blood, Circulating Tumor DNA FFPE Tissue FFPE Tissue
Species Category Human Human Human
System Compatibility NovaSeq 6000 NextSeq 500, NextSeq 550, NextSeq 550Dx in Research Mode NovaSeq 6000, NovaSeq 6000Dx in Research Mode
Technology Sequencing Sequencing Sequencing
Variant Class Copy Number Variants (CNVs), Insertions-Deletions (indels), Single Nucleotide Variants (SNVs) Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Variants (SNVs), Transcript Variants Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Variants (SNVs), Transcript Variants

Based on Pierian clinical knowledgebase, as of February 2023.

NovaSeq 6000Dx System in RUO Mode requires a separate, stand-alone DRAGEN server for secondary analysis.

NovaSeq X and NovaSeq 6000 Dx compatibility coming in 2024

Example Workflow

 

Frequently Asked Questions

The TruSight Oncology 500 ctDNA v2 is an ultra-sensitive, streamlined liquid biopsy assay for solid tumors that enables CGP from blood plasma samples in <4 days, while TruSight Oncology 500 and TruSight Oncology 500 High-Throughput enable CGP from tissue samples. See other differences in the TruSight Oncology assay comparison table.

The TruSight Oncology 500 ctDNA v2 analysis workflow uses an off-instrument software run on the DRAGEN v4 or v3 server or Illumina Connected Analytics platform to generate sample outputs, including high-level sample metrics, variants detected, and TMB and MSI scores. Tertiary analysis is enabled with either Illumina Connected Insights or the Velsera CGW.

A BaseSpace Sequence Hub evaluation app is also available for assessment use only. Illumina has no obligation to provide technical support for this app. Access is limited to 30 days.

Learn more about compatible analysis software products.

Analysis on the DRAGEN server takes approximately 20-24 hours for S4 runs with 24 libraries and approximately 9–12 hours for S2 runs with 8 libraries.

Yes, you can perform analysis with your own software. However, Illumina will not be able to directly provide technical support in this case.

Approximately 20 ng input cfDNA is recommended to achieve ~0.2% VAF detection for SNVs at >90% sensitivity and >95% specificity; however, the assay can accept a range of input from 10-30 ng cfDNA.

Case Studies

Illumina collaborates with National Cancer Center Japan to address a leading cause of death in Asia

Learn about this joint effort to accelerate the development of personalized treatments based on genomic information.

Learn more

Supporting Data and Figures

 

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