NovaSeq X Series Reagent Kits

Reagent kits for the NovaSeq X Series include a reagent cartridge, buffer cartridge, flow cell, lyo insert, pre-load buffer, and library tube strip.

For NovaSeq X Series 1.5B Reagent Kits, Illumina recommends strictly adhering to the prescribed thaw conditions and timings specified in the System Guide, not proceeding based solely on visual checks to confirm if reagents have thawed.

Overview

NovaSeq X Series Reagent Kits combine the power of XLEAP-SBS chemistry with flexible, efficient, and sustainable workflows to enable transformational science at scale.

Sustainability and simplicity in one

Improvements in XLEAP-SBS reagents allow for shipping and storage in lyophilized form, minimizing environmental impact for significant reductions in packaging size, weight, plastic mass, and waste.

The debut of XLEAP-SBS chemistry

NovaSeq X Series Reagent Kits are powered by XLEAP-SBS chemistry: a faster, more accurate, and robust update to traditional sequencing by synthesis (SBS) chemistry that supports consistent, reliable data generation for results you can trust.

Key features

  • 165 Gb to 16 Tb of highly accurate and reliable data per run to support variable batch sizes

  • 100-, 200-, and 300-cycle kits to support a wide range of applications—from spatial transcriptomics and single-cell studies to exomes and genomes

     

  • Up to 8 lanes per flow cell to simplify pooling and indexing strategies and support multiomic approaches

  • A 4-fold reduction in library input* to maximize precious samples

  • > 50% reduction in plastic† and > 90% reduction in packaging to reduce waste and maximize storage space

*Compared to NovaSeq 6000 onboard workflow

†Compared to NovaSeq 6000 Reagent Kits


Specifications


Required products

Each reagent kit contains all the required consumables for a single flow cell sequencing run. For sequencing runs requiring custom primers, Custom-Primer Buffer is available for purchase (sold separately). Spare accessories including air filters, wash flow cells, wash reagent cartridges, and sample tubes are also available for purchase (sold separately).

Each reagent kit contains all the required consumables for a single flow cell sequencing run. For sequencing runs requiring custom primers, Custom-Primer Buffer is available for purchase (sold separately). Spare accessories including air filters, wash flow cells, wash reagent cartridges, and sample tubes are also available for purchase (sold separately).

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Documentation

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Reagents (7)

NovaSeq™ X Series 25B Reagent Kit (300 Cyc)

20104706

This reagent kit provides one NovaSeq X Series 25B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 300 cycles run on the NovaSeq X and NovaSeq X Plus systems.

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NovaSeq X Series 10B Reagent Kit (300 Cycle)

20085594

This reagent kit provides one NovaSeq X Series 10B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 300 cycles run on the NovaSeq X and NovaSeq X Plus systems.

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NovaSeq X Series 10B Reagent Kit (200 Cycle)

20085595

This reagent kit provides one NovaSeq X Series 10B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 200 cycles run on the NovaSeq X and NovaSeq X Plus systems.

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NovaSeq X Series 10B Reagent Kit (100 Cycle)

20085596

This reagent kit provides one NovaSeq X Series 10B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 100 cycles run on the NovaSeq X and NovaSeq X Plus systems.

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NovaSeq™ X Series 1.5B Reagent Kit (300 Cyc)

20104705

This reagent kit provides one NovaSeq X Series 1.5B flow cell (with 2- lanes) and reagent consumables to support a single flow cell 300 cycles run on the NovaSeq X and NovaSeq X Plus systems.

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NovaSeq™ X Series 1.5B Reagent Kit (200 Cyc)

20104704

This reagent kit provides one NovaSeq X Series 1.5B flow cell (with 2- lanes) and reagent consumables to support a single flow cell 200 cycles run on the NovaSeq X and NovaSeq X Plus systems.

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NovaSeq™ X Series 1.5B Reagent Kit (100 Cyc)

20104703

This reagent kit provides one NovaSeq X Series 1.5B flow cell (with 2- lanes) with reagent consumables to support a single flow cell 100 cycles run on the NovaSeq X and NovaSeq X Plus systems.

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Accessory products (5)

NovaSeq™ X Series Custom-Primer Buffer

20100055

Buffer used for the custom primer workflow on the NovaSeq X Series. This kit is sufficient for 1 custom primer workflow for a single flow cell sequencing run.

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NOVASEQ™X Series Wash Flow Cell

20088067

NOVASEQ™X Series Wash Flow Cell is used during washes on the NovaSeq X or NovaSeq X Plus system. This part contains one wash flow cell.

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NOVASEQ™X Series Air Filter

20073109

Replacement NovaSeq X Series Air Filter for the NovaSeq X or NovaSeq X Plus system. This part contains one air filter.

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NOVASEQ™X Series Library Tube Strip (1.5B)

20072272

NOVASEQ™ X Series Library Tube Strip compatible with a NovaSeq X Series 1.5B Reagent Kit. This part contains one 1.5B (2-lane) library tube strip.

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NOVASEQ™X Series Library Tube Strip (10B/25B)

20101913

NOVASEQ™X Series Library Tube Strip compatible with a NovaSeq X Series 10B Reagent Kit or a NovaSeq X Series 25B Reagent Kit. This part contains one 10B/25B (8-lane) library tube strip.

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