These expert-defined sequencing research panels target 94 genes and 284 SNPs associated with a predisposition towards various cancers.
Assay time
Hands-on time
Input quantity
Developed in collaboration with cancer genomics experts, these predesigned, ready-to-use oligos enable researchers to sequence a variety of genes and single nucleotide polymorphisms (SNPs) previously linked to cancer predisposition.
TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.
The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatible with TruSight Rapid Capture.
6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.
| Assay time | 1.5 days |
|---|---|
| Cancer type | Pan-cancer |
| Content specifications | Fixed set of probes that enrich for 94 genes and 284 SNPs associated with common and rare cancers. |
| Description | Germline mutation detection research for common and rare cancers. |
| Hands-on time | 5 hr |
| Input quantity | 50 ng DNA |
| Instruments | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System |
| Method | Targeted DNA sequencing, Target enrichment |
| Multiplexing | Up to 96-plex |
| Nucleic acid type | DNA |
| Specialized sample types | Not FFPE-compatible, Not FFPE-compatible |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Germline variants |
You’ll also need one or more of the following enrichment products.
TruSight Cancer
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| MiniSeq System | Samples per run: mid output: 12, high output: 24 (based on > 20x coverage per target) |
Up to 2 × 150 bp |
| MiSeq System | Samples per run (by reagent kit version): v2: 12, v3: 24 (based on > 20x coverage per target) |
Up to 2 × 150 bp |
| NextSeq 550 System | Samples per run: mid output: 96, high output: 96 (based on > 20x coverage per target) |
Up to 2 × 150 bp |
Germline Mutation Detection in Cancer
Identify germline mutations that predispose individuals to cancer using next-generation sequencing and microarrays.
With targeted resequencing, a subset of genes or a genomic region is isolated and sequenced, which can conserve lab resources.
Pathology and Clinical Cancer Research
Our clinical cancer research solutions deliver accurate genomic information, and enable labs to analyze multiple genes in a single test.
Researchers from the MGZ Medical Genetic Center are using the Infinium CytoSNP-850K BeadChip and the TruSight Cancer Panel to analyze chromosomal abnormalities.
| TruSight Cancer | TruSight Hereditary Cancer Panel | AmpliSeq for Illumina BRCA Panel | |
|---|---|---|---|
| Assay time | 1.5 days | ~48 hr from DNA to data | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) |
| Cancer type | Pan-cancer | Pan-cancer, Solid tumor | Solid tumor |
| Content specifications | Fixed set of probes that enrich for 94 genes and 284 SNPs associated with common and rare cancers. | Targets 113 cancer risk-associated genes | Exonic regions and the flanking intronic sequences of the BRCA1 and BRCA2 |
| Description | Germline mutation detection research for common and rare cancers. | The TruSight Hereditary Cancer Panel is a targeted sequencing panel designed to assess germline mutations across 113 genes and 125 single nucleotide polymorphisms (SNPs) for identification purposes and polygenic risk scoring. | Germline and somatic analysis studies of BRCA1 and BRCA2. |
| Hands-on time | 5 hr | ~2 hr | <1.5 hr |
| Input quantity | 50 ng DNA | 50–1000 ng DNA | 1–100 ng (10 ng recommended per pool) |
| Instruments | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System | MiSeq System, iSeq 100 System, NextSeq 550 System, MiniSeq System, NextSeq 550Dx in Research Mode, NextSeq 500 System | MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System |
| Method | Targeted DNA sequencing, Target enrichment | Targeted DNA sequencing, Target enrichment | Targeted DNA sequencing, Amplicon sequencing |
| Multiplexing | Up to 96-plex | 96 dual index combinations | |
| Nucleic acid type | DNA | DNA | DNA |
| Specialized sample types | Not FFPE-compatible, Not FFPE-compatible | Blood, Saliva | Blood, FFPE tissue |
| Species category | Human | Human | Human |
| Technology | Sequencing | Sequencing | Sequencing |
| Variant class | Germline variants | Germline variants, Insertions-deletions (indels), Copy number variants (CNVs) | Single nucleotide polymorphisms (SNPs), Somatic variants, Germline variants, Insertions-deletions (indels) |
TruSight Cancer – Enrichment Oligos only (4 or 8 Enrichment Reactions)
FC-121-0202
TruSight Cancer targets 94 genes and 284 SNPs associated with a predisposition towards various cancers and is sufficient for 8 enrichment reactions when paired with Nextera Flex for Enrichment due to a single hybridization workflow and sufficient for 4 enrichment reactions when paired with TruSight Rapid Capture. Library prep, enrichment, and index adapter reagents need to be ordered separately.
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Illumina® DNA Prep with Enrichment, (S) Tagmentation (96 Samples)
20025524
Includes reagents for preparing and enriching 96 libraries (eight, 12-plex enrichment reactions). Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately.
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Illumina® DNA Prep with Enrichment, (S) Tagmentation (16 Samples)
20025523
Includes reagents for preparing and enriching 16 libraries (16, 1-plex enrichment reactions). Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately.
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Illumina® DNA Prep, (S) Tagmentation (96 Samples)
20025520
Includes reagents for preparing 96 libraries. No enrichment reagents are included. Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. Enrichment reagents are only orderable with library prep reagents (Illumina DNA Prep with Enrichment, (S) Tagmentation (96 samples)).
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Illumina® DNA Prep, (S) Tagmentation (16 Samples)
20025519
Includes reagents for preparing 16 libraries. No enrichment reagents are included. Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. Enrichment reagents are only orderable with library prep reagents (Illumina DNA Prep with Enrichment, (S) Tagmentation (16 samples)).
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IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)
20027213
Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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TruSight Rapid Capture (1 index, 8 samples, 8 enrichments)
FC-140-1101
Contains reagents to prepare up to 8 libraries through eight 1-plex enrichments. Includes 1 unique index combination.
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TruSight Rapid Capture (2 indexes, 8 samples, 4 enrichments)
FC-140-1102
Contains reagents to prepare up to 8 libraries through four 2-plex enrichments. Includes 2 unique index combinations.
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TruSight Rapid Capture (4 indexes, 16 samples, 4 enrichments)
FC-140-1103
Contains reagents to prepare up to 16 libraries through four 4-plex enrichments. Includes 4 unique index combinations.
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TruSight Rapid Capture (24 indexes, 48 samples, 4 enrichments)
FC-140-1104
Contains reagents to prepare up to 48 libraries through four 12-plex enrichments. Includes 24 unique index combinations.
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TruSight Rapid Capture (24 indexes, 96 samples, 8 enrichments)
FC-140-1105
Contains reagents to prepare up to 96 libraries through eight 12-plex enrichments. Includes 24 unique index combinations.
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TruSight Rapid Capture (96 indexes, 288 samples, 24 enrichments)
FC-140-1106
Contains reagents to prepare up to 288 libraries through twenty-four 12-plex enrichments. Includes 96 unique index combinations.
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Note regarding biomarker patents and other patents unique to specific uses of products:
Some genomic variants, including some nucleic acid sequences, and their use in specific applications may be protected by patents. Customers are advised to determine whether they are required to obtain licenses from the party that owns or controls such patents in order to use the product in customer's specific application.
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