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Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.
TruSight Rapid Capture all-in-one kits enable targeted sequencing library preparation and enrichment for 96 samples in 1.5 days.
Assay time
Hands-on time
Input quantity
TruSight Rapid Capture kits leverage the speed of Nextera library preparation to deliver targeted sequencing libraries. By eliminating the need for mechanical DNA fragmentation and introducing a unique multiplex pre-enrichment sample pooling, TruSight Rapid Capture enables cost-effective, high-throughput library preparation and enrichment-based sequence capture. The TruSight Rapid Capture kits offer:
Assay time | 1.5 days |
---|---|
Automation details | Explore available automation methods |
Description | A single, integrated sample preparation and enrichment workflow that generates highly sensitive indexed sequencing libraries for targeted resequencing applications from low input samples with minimal hands-on time. |
Hands-on time | 5 hr |
Input quantity | 50 ng DNA |
Instruments | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System |
Mechanism of action | Enzymatic fragmentation |
Method | Targeted DNA sequencing |
Multiplexing | Up to 96-plex |
Nucleic acid type | DNA |
Specialized sample types | Low-input samples |
Species category | Human |
Technology | Sequencing |
Variant class | Single nucleotide polymorphisms (SNPs), Germline variants |
The TruSight Rapid Capture kit generates indexed sequencing libraries that can be carried through enrichment for targeted resequencing applications.
TruSight Rapid Capture
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TruSight Rapid Capture (1 index, 8 samples, 8 enrichments)
FC-140-1101
Contains reagents to prepare up to 8 libraries through eight 1-plex enrichments. Includes 1 unique index combination.
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TruSight Rapid Capture (2 indexes, 8 samples, 4 enrichments)
FC-140-1102
Contains reagents to prepare up to 8 libraries through four 2-plex enrichments. Includes 2 unique index combinations.
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TruSight Rapid Capture (4 indexes, 16 samples, 4 enrichments)
FC-140-1103
Contains reagents to prepare up to 16 libraries through four 4-plex enrichments. Includes 4 unique index combinations.
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TruSight Rapid Capture (24 indexes, 48 samples, 4 enrichments)
FC-140-1104
Contains reagents to prepare up to 48 libraries through four 12-plex enrichments. Includes 24 unique index combinations.
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TruSight Rapid Capture (24 indexes, 96 samples, 8 enrichments)
FC-140-1105
Contains reagents to prepare up to 96 libraries through eight 12-plex enrichments. Includes 24 unique index combinations.
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TruSight Rapid Capture (96 indexes, 288 samples, 24 enrichments)
FC-140-1106
Contains reagents to prepare up to 288 libraries through twenty-four 12-plex enrichments. Includes 96 unique index combinations.
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The QuantiFluor dsDNA assay can be used to quantify dsDNA samples. The assay can quantify small DNA volumes and measure DNA directly.
50 ng of DNA is ideal for use with the TruSight Rapid Capture kit. A higher input amount can lead to incomplete tagmentation and larger insert sizes, affecting enrichment performance, while a lower input amount can generate smaller insert sizes leading to lost DNA during cleanup steps.
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