AmpliSeq for Illumina DNA Library Prep Training
Achieve unmatched data quality with AmpliSeq chemistry and Illumina next-generation sequencing (NGS) technology, even from low-quality starting materials such as formalin-fixed, paraffin-embedded (FFPE) tissues.
|AmpliSeq for Illumina Custom DNA Panel||AmpliSeq for Illumina On-Demand||Illumina DNA Prep with Enrichment|
|Content Specifications||Custom content of interest - up to 5 Mb||Custom content from 1 (24 amplicons) to 500 (15,000 amplicons) genes||Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel.
|Description||A custom research assay that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy.||A custom research assay from >5000 pretested genes that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy.||A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing.|
|Input Quantity||1–100 ng (10 ng recommended per pool)||1-100 ng DNA||10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide).|
|Mechanism of Action||Multiplex PCR||Multiplex PCR||Bead-bound transposomes and hybrid-capture chemistry|
|Method||Amplicon Sequencing, Custom Sequencing, Genotyping by Sequencing, Targeted DNA Sequencing||Amplicon Sequencing, Targeted DNA Sequencing||Custom Sequencing, Exome Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing|
|Multiplexing||Up to 96-plex||Up to 96-plex||Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested.|
|Number of Reactions||750 or 3000 reactions, 750 or 3000 reactions||24 or 96 reactions||Custom workflow: 16 or 96 samples|
|Specialized Sample Types||Blood, FFPE Tissue||Blood, FFPE Tissue||Blood, FFPE Tissue, Saliva|
|Species Category||Any Species, Bovine, Canine, Chicken, Hamster, Human, Maize, Mouse, Ovine, Porcine, Rice, Soybean, Tomato||Human||Human, Other|
Sample identification with the AmpliSeq for Illumina Sample ID Panel
Application Note | PDF < 1 MB
Illumina Adapter Sequences Documentation
AmpliSeq for Illumina Direct FFPE DNA Kit Reference Guide Documentation
AmpliSeq for Illumina Custom and Community Panels DNA Checklist Documentation
AmpliSeq for Illumina Custom and Community Panels Documentation
AmpliSeq for Illumina Custom and Community Panels RNA Checklist Documentation
AmpliSeq for Illumina Custom and Community Panels Consumables & Equipment Documentation
Index Adapters Pooling Guide Documentation
AmpliSeq for Illumina Custom and Community Panels Reference Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All AmpliSeq for Illumina Custom and Community Panels Support