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Harness the power of Illumina next-generation sequencing (NGS) technology in an easy-to-use, inexpensive ecosystem right in your lab
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Get a behind-the-scenes look at the most affordable, compact benchtop sequencing system in the Illumina portfolio and see how easy it is to harness the power of NGS in your lab.
Leveraging the speed and affordability of complementary metal-oxide-semiconductor (CMOS) technology and the accuracy of sequencing by synthesis (SBS) chemistry, the iSeq 100 System enables virtually any lab to acquire powerful NGS technology.
By providing both higher data resolution and deeper coverage compared to qPCR and Sanger sequencing, the iSeq 100 System allows greater statistical confidence for calling variants or low-frequency alleles with faster time to results and fewer hands-on steps.1,2
Compared to larger sequencing systems or outsourcing, the iSeq 100 System provides faster and cost-effective small-scale runs, independence from outsourcing, and control of the sequencing process from beginning to end.
With a maximum output of 1.2 Gb for longest supported read length, the iSeq 100 System offers rapid, multiplexed sequencing for a range of DNA and RNA sequencing applications, including small-genome sequencing, targeted gene sequencing, and gene expression profiling.
Viromics laboratory lead scientist Dr. John-Sebastian Eden from the Westmead Institute for Medical Research has brought his sequencing projects in-house. Learn why and how he decided on the iSeq 100 System.
System specifications
A comprehensive, streamlined workflow that makes the iSeq 100 System ideal for any lab seeking to perform rapid and cost-effective genetic analysis.
Manage your workflow seamlessly with Clarity LIMS software or your existing LIMS system.
Prepare libraries for a range of applications with high-performance library preparation kits.
Generate sequences with ready-to-use reagents featuring proven Illumina sequencing by synthesis chemistry in as little as 9.5 hr.
Monitor and analyze sequencing runs using the on-instrument software, Local Run Manager, and touch-screen interface.
Interpret and share results using apps located on BaseSpace Sequence Hub.
With a maximum output range of up to 1.2 Gb per run, optimized library prep kits, and high resolution and analytical sensitivity, the iSeq 100 System is a versatile option for a range of applications, including small-genome sequencing, 16S metagenomics studies, gene editing validation, and more.
Charlie Johnson, the director of genomics and bioinformatics at Texas A&M AgriLife, discusses how his lab pairs the iSeq 100 Sequencing System with the NovaSeq 6000 Sequencing System for quality control and why the iSeq 100 System has become critical to their workflow.
Proven amplicon chemistry meets industry-leading sequencing technology for cancer-related targeted resequencing applications.
iSeq 100 System |
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| Run time (range) | 9–19 hr | ~4-15.5 hr | 8–44 hr |
| Maximum output per run | 1.2 Gb | 30 Gb | 540 Gb |
| Maximum single reads per run | 4M | 100 million single reads 200 million paired-end reads |
1.8 billion single reads 3.6 billion paired-end reads |
| Data analysis offeringsb | BaseSpace Sequence Hub, Illumina Connected Analytics, on-premises DRAGEN server | On-instrument: LRM analysis modules Cloud: Broad menu of DRAGEN analysis applications available on BaseSpace Sequence Hub and Illumina Connected Analytics On-premises: Broad menu of DRAGEN analysis applications |
On-instrument: Custom menu of DRAGEN analysis pipelines Cloud: Broad menu of DRAGEN analysis applications available on BaseSpace Sequence Hub and Illumina Connected Analytics On-premises: Broad menu of DRAGEN analysis applications |
a. Max output based on 100M flow cell specifications. 50M and 100M flow cells will be available starting in 2025 for the MiSeq i100 Plus System only.
b. A dedicated DRAGEN server is paired with an Illumina sequencing system and can be used to plan secondary analysis as part of a sequencing run using the Illumina Run Manager. An on-premises DRAGEN server does not communicate directly with the sequencing system but can ingest and process data from any Illumina sequencing system via a command-line.
Reagents for the iSeq 100 System feature a ready-to-use cartridge with one-channel SBS chemistry and CMOS technology for simple, low-cost sequencing.
PhiX Control v3 is a ready-to-use control library for Illumina sequencing runs.
Offers a 90-min workflow to prepare DNA libraries for small genomes, PCR amplicons, plasmids, and cDNA sequencing, with a low DNA input requirement.
Targeted research panel investigating somatic and germline variants in BRCA1 and BRCA2.
Find educational and support resources to help you learn about next-generation sequencing (NGS) and select the right products for starting your sequencing project.
Learn how to get started with the iSeq 100 System using step-by-step guides and training videos. See how easy it can be to bring NGS to your lab.
We have taken most of the manual work out of the NGS data analysis process. Local Run Manager software is installed on every iSeq 100 System and automatically performs on-instrument data analysis. The software has a user-friendly touch-screen interface for starting a sequencing run, monitoring run status, viewing results, and analyzing data.
The iSeq 100 System is easy to install on your own. A variety of support resources are available to help you get started. Illumina scientists and engineers are at hand to provide support and training, either online or in person, to help bring laboratory personnel quickly up to speed. For long-term maintenance, the system is covered by a one-year warranty that includes a replacement instrument or field service engineer support (depending on region) to minimize downtime.
References
Get assistance with deciding if your lab can benefit from this small and powerful benchtop sequencing system. Contact us today for more information on the iSeq 100 System.
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