Prepare the lab space in advance so that you are ready to install the iSeq 100 System upon delivery. With its small footprint, the iSeq 100 System requires only minimal bench space and an electrical outlet. Use the following guidelines to set up your lab space.
How to start using the iSeq 100 System
For every lab, everywhere
Access NGS with unrivaled simplicity and unthinkable speed
Prep the lab
- Reserve bench space:
Height 42.5 cm (16.8 in) × Width 30.5 cm (12 in) × Depth 33 cm (13 in)
- Leave ample space on the left side and behind the instrument so you can access the power switch on the back panel
- Make sure you can reach the outlet easily with the provided power cord: 2.44 m (8 ft) in North America; 2.5 m (8.2 ft) in all other regions
- Use the minimum clearance dimensions specified in the site prep guide to make sure the instrument is accessible from all sides
Learn
Start exploring how to get the most out of your iSeq 100 System while you wait for it to arrive.
View educational resources about how to use the iSeq 100 System and other common next-generation sequencing topics.
Learn how to get started with the iSeq 100 System and Illumina NGS technology with our free, online training courses.
Considering switching your studies to the iSeq 100 System? See our step-by-step guides to help you transition your current workflows.
Unbox
Track your iSeq 100 System from shipment to the moment it arrives at the lab. Unpacking and installing the iSeq 100 System takes only ~30 minutes. With convenient carrying handles, the lightweight instrument can be lifted easily and placed on a lab bench.
- Shipping box weighs 21.8 kg (48 lbs)
- iSeq 100 System weighs 15.9 kg (35 lbs)
- Shipping box includes Ethernet cable, power cord, dry test cartridge, and dry test flow cell
Set up
Setting up the iSeq 100 System is as easy as connecting the power cable and turning the system on. Simply follow the onscreen prompts to perform a system check using the reusable test cartridge and flow cell.
During the system check, the iSeq 100 System will guide you through instrument qualification. You can document the results using the following protocols or through an Illumina qualification service.
Independent Qualification
Follow these step-by-step instructions to qualify the instrument independently. To receive a signed Certificate of Qualification, email the completed document to QualificationServices@illumina.com. Illumina provides this service one time per calendar year.
Illumina Qualification Service
Need help? Certified Illumina engineers can install and qualify your instrument on site. Our qualification services deliver an audit-ready report to help meet regulatory requirements.
Go
Step-by-step guidance for you and your iSeq 100 System
Prepare
Choose a library prep kit for your application, whether it’s sequencing microbes, gene panels, or custom content. You can prepare DNA or RNA libraries 1–2 days in advance, according to the manual for your library prep kit.
Compare Kits and View Protocol
| Library Prep Kit | Description | Application | Manuals & Guides | Training |
|---|---|---|---|---|
| Illumina DNA Prep | A fast, integrated workflow for sequencing amplicons, plasmids, and microbial species. | Small Whole-Genome Sequencing (microbe, virus) | Access Guide | Find Training |
| Nextera XT DNA Library Preparation Kit | Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in less than 90 minutes, with a low DNA input requirement. | Access Guide | Find Training | |
| AmpliSeq for Illumina Cancer Hotspot Panel v2 | Targeted panel investigating hotspot regions of 50 genes with known associations to cancer. | Targeted Gene Sequencing (amplicon, gene panel) | Access Guide | Find Training |
| AmpliSeq for Illumina Focus Panel | Targeted DNA and RNA panel investigating 52 genes with known relevance to solid tumors. | Access Guide | Find Training | |
| AmpliSeq for Illumina BRCA Panel | Targeted panel investigating somatic and germline variants in BRCA1 and BRCA2. | Access Guide | Find Training | |
| AmpliSeq for Illumina Immune Response Panel | Targeted RNA expression panel investigating 395 genes involved in tumor-immune system interactions. | Access Guide | Find Training | |
| AmpliSeq for Illumina Custom DNA Panel | Targeted custom panel optimized for specific targets or genomic content of interest. | Access Guide | Find Training | |
| TruSeq Small RNA | A simple, cost-effective solution for generating miRNA and small RNA sequencing libraries directly from total RNA. | Small RNA Analysis | Access Guide | Find Training |
Sequence
Starting a sequencing run on an iSeq 100 System takes only a few minutes. Simply load a cartridge and press start. Onscreen prompts will guide you through run setup.
Analyze
Make sense of your data with Local Run Manager software, installed on every iSeq 100 System. Select the analysis module for your application and the software will automatically perform data analysis.
Support
We’re always here to help. Our training courses can help you get started with library prep, sequencing, and data analysis. Illumina scientists and engineers are available globally to answer questions every step of the way.
Your iSeq 100 System will guide you through how to perform a system check and troubleshoot the instrument. The system is covered by a one-year warranty, which includes a replacement instrument or field service engineer support (depending on region) to minimize downtime.*
*Domestic shipments will arrive in as little as 1–2 days. International shipments may arrive up to 96 hours after ship time. Timing may vary depending on country and instrument condition.
See tips and best practices, online training courses, documentation, and more.
View iSeq 100 system support
Find answers to frequently asked questions about the iSeq 100 System.
Frequently asked questions
iSeq 100 System: Troubleshooting and Support
iSeq 100 System: Troubleshooting and Support
Focusing on maintenance and run optimization can help ensure maximum uptime and productivity for your iSeq 100 Sequencing System. In this video, we will highlight basic maintenance and troubleshooting activities and resources.
iSeq 100 System: Support Resources
iSeq 100 System: Support Resources
Tim Gilmartin, an Illumina Field Application Scientist, highlights the user guides, video guides, and online courses at your disposal that will help you install and use your iSeq 100 System.