微生物转录组学简介
微生物转录组和宏转录组信息对于预测特定抗生素的耐药性，了解宿主-病原体免疫相互作用，量化基因表达变化以及追踪疾病进展具有重要意义。细菌、病毒和其他微生物的新一代RNA测序（RNA-Seq）已经成为分析转录组和宏转录组信息的标准方法。
转录组是生物体基因组编码的全部RNA的集合。宏转录组为复杂样本中一群生物编码的全部RNA的集合。
用于细菌转录分析的RNA-Seq
对细菌、病毒及其他微生物进行RNA-Seq能够对广泛的微生物转录本进行注释和定量。提取细胞RNA并将其转换为cDNA，用于测序文库的制备。然后，将序列read与参考基因组进行比对，提供有关外显子连接和剪接位点等特征的定性信息，以及可与多个实验集进行比较的定量转录本数据。
与芯片等基于杂交的方法不同，RNA-Seq能够无偏差地对常见和新型转录本进行链特异性鉴定。广泛的动态范围使我们能够在单个细菌、病毒或其他微生物RNA-Seq实验中确定高表达和低表达的基因。可以使用适合自动化的一体化工作流程一次处理多个样本。
Microbial Transcriptomics with rRNA Depletion
The Ribo-Zero Plus rRNA Depletion Kit removes rRNA before RNA-Seq, enabling you to focus on high-value portions of the transcriptome. This technical note demonstrates rRNA depletion performance on microbial isolate and community samples, while comparing against previous kit versions.
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推荐的微生物转录组学工作流程
有多种方法可以开展这类实验，但工作流程的每个步骤都有一些推荐使用的产品。
点击下方的查看工作流程各步骤所使用的产品。
QuickExtract RNA Extraction Kit
用于提取贴壁细胞和悬浮细胞RNA的简便方法。
注意：如果使用TruSeq RNA文库制备试剂盒，您将不需要提取RNA并转录为cDNA。如果您使用RNA提取试剂盒，您需要生成dsDNA模板，与Nextera XT一起使用。
MasterPure Complete DNA and RNA Purification Kit
同步的DNA或RNA纯化，可用于多种应用，包括基因组文库制备。
TruSeq Stranded Total RNA Library Prep Kit
简便、经济、可扩展的总RNA分析解决方案。
注意：如果使用TruSeq RNA文库制备试剂盒，您将不需要提取RNA并转录为cDNA。
希望收到有关微生物基因组学的快讯、案例研究和信息？请输入您的电子邮箱地址。
参考文献
- Wang Z, Gerstein M, Snyder M. (2009) RNA-Seq: A revolutionary tool for transcriptomics.Nat Rev Genet 10:57–63.
- Mäder U, Nicolas P, Richard H, Bessières P, Aymerich S. (2011) Comprehensive identification and quantification of microbial transcriptomes by genome-wide unbiased methods.Curr Opin Biotechnol 22(1):32–41.
- Clyde K, Glaunsinger BA.(2011) Deep sequencing reveals direct targets of gammaherpesvirus-induced mRNA decay and suggests that multiple mechanisms govern cellular transcript escape.PLoS One 6(5):e19655.