AmpliSeq for Illumina Cancer Hotspot Panel v2

Targeted research panel investigating hotspot regions of 50 genes with known associations to cancer.Read More...
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Panel

AmpliSeq™ Cancer HotSpot Panel v2 for Illumina®

20019161


Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103


Index Adapters

AmpliSeq™ CD Indexes Set A for Illumina® (96 Indexes, 96 Samples)

20019105

配件产品

AmpliSeq™ for Illumina® Sample ID Panel

20019162

AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

AmpliSeq™ Library Equalizer for Illumina®

20019171

Product Highlights

The AmpliSeq for Illumina Cancer Hotspot Panel v2 is a targeted resequencing assay for researching somatic mutations across the hotspot regions of 50 genes with known associations to cancer, as identified in the Catalogue of Somatic Mutations in Cancer (COSMIC)1 database. Key features include:

Relevant Gene Content
  • Target ~2800 COSMIC mutations from 50 oncogenes and tumor suppressor genes
Fast, Streamlined Workflow
  • Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) samples
Accurate Data
  • Detect somatic mutations down to 5% variant allele frequency using local or cloud-based analysis

The Cancer HotSpot Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

This panel enables highly sensitive variant detection within multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
iSeq 100 System 每次运行16个样本(假设最小覆盖度为500倍) 2 × 150 bp
MiniSeq System 每次运行的样本:中通量:32,高通量:96(假设最小覆盖度为500倍) 2 × 150 bp
MiSeq System 每次运行的样本(按试剂盒版本):v2 nano:4,v2 micro:16,v2:60,v3:96(假设最小覆盖度为500倍) 2 × 150 bp

产品比较

AmpliSeq for Illumina Cancer Hotspot Panel v2 AmpliSeq for Illumina Focus Panel AmpliSeq for Illumina Comprehensive Panel v3 TruSight Tumor 15
变异种类 插入缺失, 体细胞变异, 单核苷酸多态性(SNP) 单核苷酸多态性(SNP), 基因融合, 体细胞变异, 拷贝数变异(CNV), 插入缺失 单核苷酸多态性(SNP), 基因融合, 体细胞变异, 拷贝数变异(CNV), 插入缺失 体细胞变异, 插入缺失
技术 测序 测序 测序 测序
方法 靶向DNA测序 , 扩增子测序 靶向DNA测序 , 靶向RNA测序 , 扩增子测序 靶向DNA测序 , 靶向RNA测序 , 扩增子测序 扩增子测序 , 靶向DNA测序
核酸类型 DNA DNA, RNA DNA, RNA DNA
物种类别 人类 人类 人类 人类
特殊的样本类型 血液, FFPE组织 血液, FFPE组织 血液, FFPE组织 FFPE组织
癌症类型 泛癌, 实体瘤 实体瘤 实体瘤 实体瘤
系统兼容性 研究模式下的MiSeqDx , MiniSeq , MiSeq , MiSeq MiniSeq , MiSeq , MiSeq, 研究模式下的MiSeqDx NextSeq 550 MiniSeq , 研究模式下的MiSeqDx , MiSeq , MiSeq
起始量 1–100 ng(建议每个混池10 ng) 1–100 ng(建议每个混池10 ng) 1–100 ng(建议每个混池10 ng) 20 ng

Method-Specific Workflow Example

 

Related Products

AmpliSeq for Illumina Focus Panel

Targeted DNA and RNA research panel investigating 52 genes with known relevance to solid tumors.


TruSight Tumor 15

Focused sequencing panel to assess 15 genes that are commonly mutated in solid tumors in a single assay, with a simple, rapid workflow.


AmpliSeq for Illumina Custom DNA Panel

Targeted custom research panels optimized for sequencing specific targets or genomic content of interest.


References
  1. Kingsmore S. Catalogue of Somatic Mutations in Cancer – Home Page. http://cancer.sanger.ac.uk/cosmic. Accessed October 25, 2017.