TruSeq Methyl Capture EPIC Library Prep Kit

This kit combines next-generation sequencing with epigenetic insights to accelerate biomarker discovery and understand methylation’s role in gene regulation.Read More...
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TruSeq-Methyl Capture EPIC Library Prep Kit (12 samples, 4 indexes)

FC-151-1002

TruSeq-Methyl Capture EPIC Library Prep Kit (48 samples, 12 indexes)

FC-151-1003

Product Highlights

Targeted methylation sequencing (Methyl-Seq) offers a balanced, cost-effective choice between whole-genome bisulfite sequencing and methylation arrays that can support both screening and biomarker discovery study objectives.1 TruSeq Methyl Capture EPIC Library Prep supports researchers seeking a deeper understanding of the role of methylation in gene regulation.

Comprehensive methylation-site content: Offers significantly higher coverage of emerging epigenetic regions of interest compared to other Methyl-Seq kits currently on the market (See Supporting Data section, Epigenetic Regions Covered).

Maximize discovery power, minimize cost: Efficiently query differentially methylated bases to conserve research dollars.

Convenient kit configuration: Includes bisulfite conversion, library prep, target enrichment, and purification reagents in a single kit for hassle-free ordering.

User-friendly data analysis: Analyze sequence data in BaseSpace Sequence Hub with methylation apps designed for biologists.

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
NextSeq 550 System 每次运行8个样本(高通量;基于40倍平均覆盖度和90%以上的目标碱基覆盖度≥10倍) 2 x 100 bp (最大推荐值)
NovaSeq 6000 System 每次运行的样本(双流动槽):S1:48,S2:48,S4:96(受可用标签组合的限制;基于>5500万条read) 2 × 100 bp (最大推荐值)
HiSeq 2500 System 每次运行的样本:快速运行:8–12,高通量v4:64(基于40倍平均覆盖度和90%以上的目标碱基覆盖度≥10倍) 2 × 100 bp (最大推荐值)
HiSeq 3000 System 每次运行48个样本(高通量;基于40倍平均覆盖度和90%以上的目标碱基覆盖度≥10倍) 2 × 100 bp (最大推荐值)
HiSeq 4000 System 每次运行96个样本(高通量;基于40倍平均覆盖度和90%以上的目标碱基覆盖度≥10倍) 2 × 100 bp (最大推荐值)

产品比较

TruSeq Methyl Capture EPIC Library Prep Kit Infinium MethylationEPIC Kit
变异种类 差异甲基化胞嘧啶 差异甲基化胞嘧啶
技术 测序 微阵列芯片
核酸类型 DNA DNA
物种类别 人类 人类
系统兼容性 NextSeq 500 , HiSeq 2500 , NextSeq 550 , HiSeq 3000 , HiSeq 4000 iScan
起始量 500 ng人类基因组DNA 250 ng DNA

Method-Specific Workflow Example

 

Supporting Data and Figures

 

This kit requires a PCR enzyme kit such as the Kapa Hifi Uracil+ (Cat No. KK2801), available separately.

Related Products

Infinium MethylationEPIC Kit

Robust methylation profiling microarray with unparalleled coverage of CpG islands, genes, and enhancers. Use for epigenome-wide association studies.


AmpliSeq for Illumina Transcriptome Human Gene Expression Panel

Targeted panel that measures expression levels of >20,000 human RefSeq genes.


AmpliSeq for Illumina Comprehensive Cancer Panel

Targeted panel investigating the exonic regions of 409 genes with known associations to cancer.


References
  1. Masser DR, Stanford DR, and Freeman WM. Targeted DNA methylation analysis by next-generation sequencing. J Vis Exp. 2015; (96).
  2. Agilent SureSelect MethylSeq target regions. Accessed 14 June 2016.
  3. Roche Nimblegen CpGiant target regions. Accessed 14 June 2016.