AmpliSeq for Illumina Comprehensive Panel v3
Targeted DNA and RNA research panel investigating variants across 161 genes associated with a range of cancer types.Read More...
The AmpliSeq for Illumina Comprehensive Panel v3 offers coverage of key cancer genes, including kinase domains and genes involved in DNA repair.
The Comprehensive Panel v3 is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
The panel content spans hotspot regions, full-length genes*, copy number genes, and inter- and intragenic gene fusions. This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| MiniSeq System | 3 samples per run (high output; assumes minimum coverage of 500×) | 2 × 150 bp |
| MiSeq System | 3 samples per run with v3 reagents (assumes minimum coverage of 500×) | 2 × 150 bp |
| NextSeq 550 System | Samples per run: mid output: 16, high output: 48 (assumes minimum coverage of 500×) | 2 × 150 bp |
| AmpliSeq for Illumina Comprehensive Panel v3 | TruSight Tumor 170 | TruSight Tumor 15 | AmpliSeq for Illumina Focus Panel | AmpliSeq for Illumina Cancer Hotspot Panel v2 | |
|---|---|---|---|---|---|
| Assay Time | 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 7 hours | 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | |
| Cancer Type | Solid Tumor | Solid Tumor | Solid Tumor | Solid Tumor | Solid Tumor, 泛癌种 |
| Content Specifications | DNA and RNA targets for 161 oncogenes | Amplifies 250 amplicons from 15 genes associated with solid tumors | DNA and RNA targets for 52 oncogenes | Hotspot regions of 50 genes with known associations to cancer | |
| Description | Somatic analysis research on hotspot and full-length targets of genes associated with solid tumor cancers. | Focused panel to study relevant solid tumor somatic variants in 15 genes using a simple workflow. | Somatic analysis research on 52 genes associated with solid tumor cancers. | Somatic analysis research into hotspot regions of 50 cancer-related genes. | |
| Hands-On Time | < 1.5 hours | 3.5 hours | < 1.5 hours | < 1.5 hours | |
| Input Quantity | 1–100 ng (10 ng recommended per pool) | 20 ng | 1–100 ng (10 ng recommended per pool) | 1–100 ng (10 ng recommended per pool) | |
| Method | Amplicon Sequencing , 靶向DNA测序 , 靶向RNA测序 | 靶向DNA测序 , 靶向RNA测序 , 靶向富集 | Amplicon Sequencing , 靶向DNA测序 | Amplicon Sequencing , 靶向DNA测序 , 靶向RNA测序 | Amplicon Sequencing , 靶向DNA测序 |
| Multiplexing | 96 dual index combinations | 1-24-plex | 96 dual index combinations | 96 dual index combinations | |
| Nucleic Acid Type | DNA, RNA | DNA, RNA | DNA | DNA, RNA | DNA |
| Specialized Sample Types | FFPE Tissue | FFPE Tissue, Low-Input Samples | FFPE Tissue | FFPE Tissue | FFPE Tissue |
| Species Category | 人类 | 人类 | 人类 | 人类 | 人类 |
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
Data Sheet | HTML | 5 versions
Ampliseq for Illumina Comprehensive Panel v3 Gene List
product_file | EXCEL< 1 MB
NextSeq 550 amplicon sequencing solution
Application Note | PDF< 1 MB
Illumina Adapter Sequences Documentation
Index Adapters Pooling Guide Documentation
AmpliSeq for Illumina Direct FFPE DNA Kit Reference Guide Documentation
AmpliSeq for Illumina Comprehensive Panel v3 Reference Guide Documentation
AmpliSeq for Illumina Comprehensive Panel v3 Support - Documentation & Literature
AmpliSeq for Illumina Comprehensive Panel v3 Checklist Documentation
自定义协议选择器
Generates customized, end-to-end instructions
Targeted panel that measures expression levels of >20,000 human RefSeq genes.
