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Simple, accessible sequencing for targeted DNA and RNA applications
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We are making the MiniSeq Sequencing System obsolete. It will be available for order until September 30, 2025. We will continue to provide full system support through December 31, 2029. For more information, refer to PON2025-1657. The MiSeq i100 Series is the recommended alternative.
Learn how professors at Webster University harness the easy-to-use MiniSeq System to unlock new research possibilities and foster faculty and student collaborations.
The MiniSeq System delivers the power and confidence of proven Illumina next-generation sequencing (NGS) technology in an accessible sequencing solution. The MiniSeq System features:
The MiniSeq System combines load-and-go operation with an intuitive user interface for a fast, easy NGS workflow. The MiniSeq System integrates clonal amplification, sequencing by synthesis, and base calling into a single benchtop tool, eliminating the need for additional equipment. Simply load the flow cell and reagent cartridge into the MiniSeq System and sequence at the touch of a button.
The MiniSeq System features a touch screen interface and user-friendly, intuitive software. Configure, launch, monitor, and analyze sequencing runs with onboard Local Run Manager. Interface with the MiniSeq System from any web browser to review real-time data and performance metrics.
Perform secure analysis of MiniSeq System data in the cloud with BaseSpace Sequence Hub. Access accelerated bioinformatics with push-button tools, enabling you to easily analyze, archive, and share sequencing data. Optimized data analysis workflows are available for a growing ecosystem of applications.
Examine single genes or entire pathways—all in a single run. Get efficient sequencing across a range of applications and sample quantities to help answer a myriad of research questions.
System specifications
Find insights with an integrated workflow requiring minimal hands-on time.
Prepare DNA or RNA libraries for a diverse range of targeted applications using optimized library prep kits.
Load and set up a run in < 5 minutes with an intuitive user interface and simple operation. Complete sequencing in < 1 day.
Configure, launch, monitor, and analyze sequencing runs using the onboard software suite.
Interface with the MiniSeq System from any web browser to review real-time data and performance metrics.
The MiniSeq System enables researchers to transition easily between sequencing projects for both DNA and RNA applications, including targeted resequencing, expression profiling, tumor profiling, germline mutation analysis, and more.
MiniSeq System |
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| Run time | 4–24 hr | ~4-15.5 hr | 8–44 hr |
| Maximum output per run | 7.5 Gb | 30 Gb | 540 Gb |
| Maximum single reads per run | 25Ma | 100 million single reads 200 million paired-end reads |
1.8 billion single reads 3.6 billion paired-end reads |
| Data analysis offeringsb | BaseSpace Sequence Hub, Illumina Connected Analytics, on-premises DRAGEN server | On-instrument: LRM analysis modules Cloud: Broad menu of DRAGEN analysis applications available on BaseSpace Sequence Hub and Illumina Connected Analytics On-premises: Broad menu of DRAGEN analysis applications |
On-instrument: Custom menu of DRAGEN analysis pipelines Cloud: Broad menu of DRAGEN analysis applications available on BaseSpace Sequence Hub and Illumina Connected Analytics On-premises: Broad menu of DRAGEN analysis applications |
a. For MiniSeq High Output Reagent Kit only.
b. A dedicated DRAGEN server is paired with an Illumina sequencing system and can be used to plan secondary analysis as part of a sequencing run using the Illumina Run Manager. An on-premises DRAGEN server does not communicate directly with the sequencing system but can ingest and process data from any Illumina sequencing system via a command-line.
MiniSeq System Reagent kits have a ready-to-use cartridge with two-channel SBS chemistry for cost-efficient sequencing, even for low sample numbers.
Targeted sequencing panel that assesses germline mutations across 113 genes and 125 SNPs associated with genetic cancer risk predisposition.
Library prep and enrichment kit that delivers highly sensitive, comprehensive respiratory pathogen identification and antimicrobial resistance insights.
A library prep and enrichment kit that enables sensitive, culture-free detection and analysis of common and underrecognized uropathogens and AMR genes.
Ready to speak to someone? Contact us to learn if the MiniSeq System is right for your research needs.
Your email address is never shared with third parties.
