A targeted BRCA1/2 gene panel, utilizing the single-tube SLIMamp workflow for fast library prep and turnaround time.
The Pillar oncoReveal BRCA1 & BRCA2 + CNV Panel targets BRCA1/2 mutations and copy number variations. The assay uses proprietary Stem Loop Inhibition-Mediated amplification (SLIMamp) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.
| Content specifications | Covers full coding gene sequence, plus flanking intronic regions (10bp) |
|---|---|
| Instruments | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode, MiSeq i100 Plus System, MiSeq i100 System |
| Method | Targeted DNA sequencing, Amplicon sequencing |
| Nucleic acid type | DNA |
| Specialized sample types | Blood, Low-input samples, FFPE tissue |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Somatic variants, Germline variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) |
Pillar oncoReveal panels require the use of a Pillar index kit.
Pillar oncoReveal panels require the use of a Pillar index kit. Pick your preferred number of reactions.
Pillar oncoReveal BRCA1 & BRCA2 + CNV Panel
| Pillar oncoReveal BRCA1 & BRCA2 + CNV Panel | AmpliSeq for Illumina BRCA Panel | |
|---|---|---|
| Content specifications | Covers full coding gene sequence, plus flanking intronic regions (10bp) | Exonic regions and the flanking intronic sequences of the BRCA1 and BRCA2 |
| Instruments | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode, MiSeq i100 Plus System, MiSeq i100 System | MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System |
| Method | Targeted DNA sequencing, Amplicon sequencing | Targeted DNA sequencing, Amplicon sequencing |
| Nucleic acid type | DNA | DNA |
| Specialized sample types | Blood, Low-input samples, FFPE tissue | Blood, FFPE tissue |
| Species category | Human | Human |
| Technology | Sequencing | Sequencing |
| Variant class | Somatic variants, Germline variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) | Single nucleotide polymorphisms (SNPs), Somatic variants, Germline variants, Insertions-deletions (indels) |
Pillar oncoReveal BRCA1 & BRCA2 Plus CNV Panel specifications for the detection of DNA variants from BRCA 1 and BRCA2.
Pillar® oncoReveal™ BRCA1 & BRCA2 + CNV Panel (24 reactions)
HDA-BR-1003-24
Pillar® oncoReveal™ BRCA1 & BRCA2 + CNV Panel (24 reactions)
Indices PI501-8, PI701-4 (32 combinations, 96 reactions)
IDX-PI-1001-96
Indices PI501-8, PI701-4 (32 combinations, 96 reactions)
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Kit D, indices PI501-8, PI701-12
IDX-PI-1004-192
Kit D, indices PI501-8, PI701-12
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