Infinium XT iSelect-96 Kit

A comprehensive microarray solution that enables production-scale genotyping of up to 100,000 single- or multi-species custom variants for non-human, and 50,000 custom variants for human.

> 1M samples/year max. Range: 100K to > 1M samples/year.

Sample throughput

96 samples per array

Number of samples

50K custom markers

Number of markers

See full details in the specifications table

Overview

The Infinium XT iSelect-96 Kit provides a high-throughput genotyping solution that enables large-scale screening applications on any species with user-defined content. Applications such as large-scale genetic improvement programs in agrigenomics or large-scale screening for biobanks and personalized medicine initiatives can benefit from the accuracy and robustness of the Infinium XT solution.  

  • Production-scale genotyping—Optimized assay and 96-sample BeadChip enables processing of hundreds of thousands to more than one million samples per year

  • Flexible content—Targeted single or multispecies assays of hundreds to 50K SNPs with at least 95% conversion rate guaranteed for custom panels

  • High-efficiency workflow—Total assay turnaround time reduced from 3 days to 2 days with less hands-on time

Streamlined sample preparation and data analysis

The fully automated, highly efficient Infinium XT workflow streamlines genotyping sample preparation and analysis. Updates to the Illumina Automation Control (IAC) software and integration of LIMS increases sample processing efficiency while enhancements to GenomeStudio and Beeline Software offer real-time data generation and on-demand QC report functionality. 


Specifications


Required products

The Infinium XT iSelect-96 Kit includes BeadChips and reagents only. Each kit is processed as a single batch and cannot be divided across multiple experiments. Order multiple small kits for processing several low-throughput batches.

Required: 

  • Infinium XT Starter and Upgrade Kits—Hardware and accessories to run the Infinium XT assay for new users (starter) and existing Infinium users new to XT workflow (upgrade)

  • Infinium XT Assay PB20 Kit—Concentrated reagent buffer  

  • iScan System—Scanner and accessories for imaging

Optional: 

/ Results

Applications

The Infinium XT iSelect-96 Kit features a 96-sample BeadChip that supports ≤ 50K customizable markers, enabling large-scale genotyping applications, including genetic improvement programs in agrigenomics and large-scale screening for biobanks and personalized medicine initiatives.

Example workflow


Related applications and methods

Documentation

Product literature

Compare

Infinium XT iSelect-96 Kit Infinium HTS iSelect Custom Beadchips Infinium Global Screening Array-24 Kit BovineSNP50 v3 DNA Analysis BeadChip
Assay type Infinium XT Infinium HTS Infinium HTS Infinium HTS
Automation capability Liquid handling robots, Automated array loader Liquid handling robots, Automated array loader Liquid handling robots, Automated array loader Liquid handling robots, Automated array loader
Description

The Infinium XT iSelect-96 Kit enables cost-effective genotyping for large-scale studies on virtually any species with a throughput range of 50K to > 1M samples/year. The BeadChip supports up to 100K nonhuman and 50K human markers with prevalidated or novel custom content.

The Infinium HTS iSelect Custom BeadChip enables researchers to design a fully customized high-throughput screening panel to interrogate virtually any marker in the genome for virtually any species to fit their research needs. 

The Infinium Global Screening Array Kit is an economical genotyping array for population-scale genetics, translational research, variant screening studies, and precision medicine research, combining optimized multiethnic genome-wide content, curated clinical research variants, and QC markers.

Input quantity 200 ng DNA 200 ng DNA 200 ng DNA 200 ng DNA (at 150 ng/ul)
Instruments iScan System iScan System iScan System iScan System
Method High-throughput genotyping array, Custom array Custom array High-throughput genotyping array, Genome-wide genotyping array Genome-wide genotyping array
Nucleic acid type DNA DNA DNA DNA
Number of markers 50K custom markers 700K custom markers max

654,027 fixed markers. Custom marker add-on capacity: Up to 100K. 

Fixed markers: 53,218 evenly distributed SNPs across the genome
Custom marker add-on capacity: Up to 600,000 (with + kit versions)
Number of samples

96 samples per array

24 samples per array 24 samples per array
Sample throughput > 1M samples/year max. Range: 100K to > 1M samples/year. 5760 samples per week max (with 2 iScan systems, 1 AutoLoader 2x, and 2 Tecan robots over an 8-hour day) ~5760 samples per week
Species category Drosophila, Any species, Shrimp, Mammalian, Tomato, Bovine, Mouse, Canine, Yeast, Rice, Zebrafish, Ovine, Hamster, Chicken, Porcine, Human, Rat, Equine, Plant, Virus, Maize, Nematode, Bacteria, Soybean Drosophila, Any species, Shrimp, Mammalian, Tomato, Bovine, Mouse, Canine, Yeast, Rice, Zebrafish, Ovine, Hamster, Chicken, Porcine, Human, Rat, Equine, Plant, Virus, Maize, Nematode, Bacteria, Soybean Human Bovine
Technology Microarray Microarray Microarray Microarray
Variant class Single nucleotide polymorphisms (SNPs), Germline variants, Insertions-deletions (indels) Single nucleotide polymorphisms (SNPs), Germline variants, Structural variants, Insertions-deletions (indels), Copy number variants (CNVs) Single nucleotide polymorphisms (SNPs), Structural variants, Copy number variants (CNVs)

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Figures

infinium xt workflow improvements

Infinium XT iSelect-96 Kit workflow improvements

Each step is optimized in the Infinium XT iSelect-96 Kit workflow, reducing the turnaround time from 3 days to 2 days.

Resources

Thumbnail

Infinium genotyping: Introduction to DesignStudio Microarray Assay Designer

Custom content (9)

Infinium® XT iSelect-96 Kit (1152 samples)

20006613

This kit contains 12 custom BeadChips and tube-based reagents to process 1152 samples in 4, 8 or 12 BeadChip batches through the Infinium XT-96 Semi-High Throughput (ST) Manual or Automated workflow.

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Infinium XT iSelect-96 Kit (4608 samples)

20006614

This kit contains 48 custom BeadChips and plate-based reagents to process 4,608 samples in 24 or 48 BeadChip batches through the Infinium XT-96 High-Throughput (HT) and Ultra High-Throughput (UHT) workflows.

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Infinium XT iSelect-96 Kit (23040 samples)

20006615

This kit contains 240 custom BeadChips and plate-based reagents to process 23,040 samples in 24 or 48 BeadChip batches through the Infinium XT-96 High-Throughput (HT) and Ultra High-Throughput (UHT) workflows.

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Infinium XT Starter Kit (48 BeadChip batches)

20011069

This kit supports new-to-Infinium customers looking to perform targeted genotyping at a high-throughput level utilizing the Infinium XT workflow. It includes accessory equipment required to process 24 BeadChip batches through the Infinium XT workflow. Tip Guides, iScan, AutoLoader 2.x and automation robot sold separately.

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Infinium® XT Starter Kit (24 Beadchips)

20011100

Each Infinium XT Starter Kit contains all the accessory hardware needed for processing 24 BeadChip batches.

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Infinium XT Starter Kit (24 Beadchip) 220V

20031992

Infinium XT Starter Kit (24 Beadchip) 220V

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Infinium XT Starter Kit (24 Beadchip) 110V

20031915

Infinium XT Starter Kit (24 Beadchip) 110V

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Infinium XT Starter Kit (48 Beadchip) 110V

20031916

Infinium XT Starter Kit (48 Beadchip) 110V

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Infinium XT Starter Kit (48 Beadchip) 220V

20031993

Infinium XT Starter Kit (48 Beadchip) 220V

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Automation (4)

AutoLoader 2.x, Single-Scanner Configuration (110/220 V)

SY-202-1001

Includes one AutoLoader 2.x robot, five hotels, 10 black carriers, one AutoLoader 2.x baseplate, one iScan baseplate, installation, Illumina AutoLoader software, and one-year standard warranty to support one iScan System. iScan System sold separately.

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AutoLoader 2.x, Dual-Scanner Configuration (110/220 V)

SY-202-1002

This kit supports customers who want to maximize utilization of their iScans and sample throughput capacity by enabling automated loading of two iScans. It includes one AutoLoader robot, five hotels, twenty carriers, baseplates, installation and the Illumina AutoLoader software. iScan sold separately. One-year standard warranty.

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Infinium Automated Pipetting System with IAC (110V)

SC-30-403

This package supports customers who want to automate their Infinium processing and integrate a LIMS system (sold separately). It includes a LIMS-ready Tecan Freedom EVO 150cm liquid handler with RoMA ARM equipped to run the Pre-AMP OR Post-AMP Infinium Assay protocols, robotic accessories and Robot Control Software. LIMS server, software and license sold separately.

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Infinium Automated Pipetting System with IAC (220V)

SC-30-404

This package supports customers who want to automate their Infinium processing and integrate a LIMS system (sold separately). It includes a LIMS-ready Tecan Freedom EVO 150cm liquid handler with RoMA ARM equipped to run the Pre-AMP OR Post-AMP Infinium Assay protocols, robotic accessories and Robot Control Software. LIMS server, software and license sold separately. NOTE: This does not include the Te-Flow Rack/Water Circulator kit, which is required for the Infinium workflow and must be ordered separately (PN 20028404)

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Instrument (1)

iScan® System (110/220V)

SY-101-1001

Includes the iScan Reader, isolation table, computer, installation, and 12 months warranty to support imagining of Infinium BeadArray products. For use in locations with 100–120 V/220–240 V, 50–60 Hz voltage.

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Accessory products (9)

Infinium® XT Assay PB20 Kit

20007420

Concentrated (bulk) reagent buffer for the Infinium XT assay workflow.

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Infinium® XT Upgrade Kit (24 Beadchips)

20011101

Each Infinium XT Upgrade Kit contains all the accessory hardware specific to processing 96-XT BeadChips in 24 BeadChip batch sizes.

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Infinium XT ST Upgrade Kit (Up to 12 BeadChip Batches)

20015526

Each Infinium XT ST Upgrade Kit contains all the accessory hardware needed for processing up to 12 BeadChip batches using the ST Automated or Manual workflow. Intended for customers already running the Infinium Assay and upgrading their lab to incorporate Infinium XT ST workflow.

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Infinium® 96-XT Tip Guide Set

20011102

Each Infinium 96-XT Tip Guide Set comes with 3 sets of 3 Tip Guides used to hybridize 96 samples to each BeadChip.

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XT Dual Hyb Insert and Baseplate

20011759

Infinium XT Hybridization Chamber inserts. These parts have a dual purpose as chamber assembly inserts and a base plate for the 96-XT BeadChips on Tecan during the sample hybridization process.

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XT Flow-Through Chamber Assembly Tray

20011760

Tray supporting the Infinium 96-XT BeadChips during the flow through assembly process.

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Infinium® XT HT Workflow Training Kit, Reagents Only

20012126

Contains plate-based reagents only for processing the Infinium XT HT workflow for Illumina onsite training purposes only. BeadChips must be ordered separately – specify BeadChip part number in the comments section and work with customer service to place your order.

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XT Hyb Chamber Gasket

20012127

Gasket insert for Infinium XT Hybridization Chamber supporting the hybridization of DNA samples to BeadChips

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XT Flow Through Chamber (8 pack)

20012129

XT Flow Through Chamber (8 pack)

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FAQs

Key features of the Infinium XT workflow include:

  • DNA amplification—Increased batch size three-fold and reduced incubation time 

  • Enzymatic fragmentation—Decreased time by 50%

  • Alcohol precipitation—Removed a 30-minute incubation step and reduced drying time by 75% 

  • DNA resuspension—Reduced overall incubation time by 75%

  • BeadChip hybridization—Increased sample capacity three-fold using the Tecan robotic system 

  • Sample extension/staining—Increased capacity two-fold; 4608 samples per run

GenomeStudio Software is the Illumina visualization and analysis program for microarray-based genotyping data. Important updates to the software increase the speed of genotype cluster generation, reducing overall analysis turnaround time. 

 

Beeline Software is the Illumina microarray data analysis tool for prefiltering and reporting data from high-throughput processing. This makes it ideal for any large-scale Infinium genotyping project.

DesignStudio Software includes a custom genotyping microarray probe designer whereby custom content can be developed and ordered through a guided, easy-to-use interface with a guarantee of at least a 95% custom content conversion rate.

Infinium XT iSelect-96 Kit can be processed with current Infinium automation (ie Tecan robots, LIMS, and assay accessory hardware). Optimizations to the IAC software for the Tecan liquid handling robot increase efficiency and save time with pre-PCR liquid transfer steps while updates to the LIMS software support the entire process. Some accessory hardware updates will be required to support the new format and reduce hands-on time. 

The Infinium XT iSelect-96 Kit solution is designed and optimized to support customers looking to scale their Infinium labs using an automated workflow. Customers must batch 96 samples to process one BeadChip and at least 1152 samples per run to optimize the reagent usage. Labs that process < 50K samples per year may extend the end-user turnaround time and may not justify the capital expenses from procuring automation. 
/ Results

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