NovaSeq X Series: optimized for comprehensive omic solutions

Expand biological discovery with the power of high-throughput multiomics 

Harness omic solutions with the NovaSeq X Series 

The NovaSeq X Series delivers the scalable sequencing power and flexibility to support a broad range of methods for interrogating the genome, epigenome, transcriptome, and proteome. Engineered to output data with the highest integrity, the NovaSeq X Series makes even the most data-intensive research accessible with industry leading speed and throughput.

Unleash discovery power across multiple omes with innovations designed for the NovaSeq X Series:

Flexibility

Dual-flow cell architecture and up to 16 individually addressable lanes support broad omic products and applications. Powerful software portfolio from Illumina streamlines data analysis and interpretation across diverse workflows.  

Scalability

Multiple cycle kits support a wide range of scalable options. The NovaSeq X Plus System and 1.5B, 10B, and 25B flow cells enable data-intensive multiomic studies at a broader and deeper scale than ever before.

Efficiency

Reduced touch points and steps allow faster and easier workflows from sample preparation to data interpretation. Individually addressable lanes help tailor sequencing data per library.

Cost

Process more samples and access more omes with up to 3× higher accuracy.1 The 25B flow cell increases the number of samples by up to 2.5× for the same budget.2

  1. With XLEAP-SBS Chemistry
  2. Compared to the NovaSeq 6000 S4 flow cell.
Novaseq-X open

Omic methods and workflows with the NovaSeq X Series

The NovaSeq X Series makes multiomics accessible to enable discoveries that were previously out of reach. Explore products and reagents, with supporting application notes, resources, and relevant analysis solutions to interpret results for omics workflows below. 

By studying the genome, researchers can use information about the structure, function, and sequence of DNA to better understand its role in health and disease.

Featured solutions and resources

Illumina DNA Prep

A fast, robust, and flexible workflow for a wide range of DNA input types.

The transcriptome includes all RNA molecules in an organism and is an important area of study to understand the function of the genome, which can reveal insights into diseases and drug discoveries.

Featured solutions and resources

Illumina RNA Prep with Enrichment

A fast, integrated workflow for producing enriched and indexed sequencing libraries from a broad range of sample types and RNA input quantities. Learn more in our App Note.

Illumina Stranded mRNA Prep

A streamlined solution for clear and comprehensive analysis across the coding transcriptome for discovery of features such as novel isoforms, gene fusions, and allele-specific expression. Learn more in our Data Sheet.

Analysis for single-cell RNA sequencing

Read how the Illumina BaseSpace Sequence Hub now supports ScaleBio’s Single Cell RNA Sequencing Kit, a combinatorial indexing and instrument-free approach to achieve highly multiplexed library preparation on hundreds of thousands of cells for single cell RNA analysis.

Epigenomics is the complete study of epigenetic changes of many genes within a cell or organism other than DNA sequence changes.

Featured solutions and resources

Proteomics is the complete study of the structure and function of proteins in an organism. The development of high-throughput proteomics using NGS can advance biomarker discoveries and improve our understanding of cellular functions and diseases. 

Featured solutions and resources

Combine protein and gene expression profiling

In this Application Note, see how to use methods such as BEN-Seq (bulk epitope and nucleic acid sequencing) and CITE-Seq (cellular indexing of transcriptomes and epitopes sequencing) to analyze protein and gene expression profiling in Th1 cells.

Analyzing CITE-Seq data

Learn how to leverage the DRAGEN Single-Cell RNA app on the Illumina BaseSpace Sequence Hub to analyze multiplexed samples (mRNA and protein).

Illumina Protein Prep 

Aptamer-based proteomics assay to identify and quantify ~6000 unique human proteins. Currently in an early access, limited release. Available in 2025. 

Omics data analysis and interpretation solutions

Illumina offers a comprehensive portfolio of software solutions to analyze omic data and lead to novel insights. Explore informatic solutions capable of scaling to production-level systems such as the NovaSeq X Series to streamline data to insight with efficiency and ease.

Software solutions

DRAGEN secondary analysis

Get accurate, comprehensive, and highly efficient bioinformatics with multiple deployment options, applications, and pipelines to meet your research needs.  

BaseSpace Sequence Hub

Integrates with Illumina sequencing systems with an intuitive interface for streamlined run setups and monitoring. Enjoy intuitive push-button secondary analysis and data sharing within a secure cloud ecosystem with expandable storage for growing laboratory needs.

Illumina Connected Analytics

A secure, scalable, bioinformatics platform that enables you to manage, analyze, and interpret large volumes of multiomic data. 

Partek Flow analysis solution

Partek Flow bioinformatics software provides a visual user interface, robust statistical algorithms, information-rich visualizations, and cutting-edge genomic tools that enable researchers of all skill levels to confidently perform data analysis. 

Software articles and resources

DRAGEN for single-cell genomic insights

Read this update on the introduction of innovative pipelines for DRAGEN, including single-cell ATAC-seq and single-cell multiomics (RNA-Seq and ATAC-seq).

BaseSpace Sequence Hub with 10x Genomics libraries

Learn how sequencing runs with 10x Genomics libraries can now be directly set up on the Run Planner. The Run Planner tool guides you through the steps needed for preparing your sequencing run, such as specifying sample and index information, read length and analysis options.

Partek bioinformatics for spatial multiomics

Learn how to combine RNA and protein data, statistically assess relationships using correlation between gene and protein molecules, and explore differences between cell types using differential analysis comparisons for biological interpretation.

Thumbnail
Illumina Connected Analytics for multiomic cancer applications

A secure, scalable, bioinformatics platform that enables you to manage, analyze, and interpret large volumes of multiomic data. 

Multiomics eBooks

multiomics

See how you can use multiomics to better connect genotype to phenotype and obtain a full cellular readout not found through single omics approaches.

multiomics sequencing

With the NovaSeq X Series, learn how you can perform broader, deeper sequencing and take on omics projects that were previously out of reach.

Gene expression

This eBook outlines the NGS analysis workflow and provides an overview of Illumina Connected Software solutions available for analyzing gene expression and regulation data.

Single cell sequencing

See how you can use multiomics to better connect genotype to phenotype and obtain a full cellular readout not found through single omics approaches.

Additional resources

Accelerate omics pattern discovery using Correlation Engine

Read how Illumina’s Correlation Engine provides a curated omics knowledge base of biological interactions that is driven by the vast amount of multiomic public data and publications, along with the built-in comprehensive analytic correlation tools.

FAQs

You can learn more at our multiomics page where you’ll see how to expand your research and discovery power across multiple omes.

The NovaSeq X Series is capable of generating large volumes of valuable data which can be analyzed using Illumina Connected Analytics (ICA) for production-scale informatics. ICA provides tight integration with Illumina hardware and software with customizable workflows and built-in security and compliance features to meet regulated environments. In addition, BaseSpace Sequence Hub offers an intuitive interface that allows you to streamline run setups and monitoring using a secure, cloud-based ecosystem suited for growing laboratories.

A variety of solutions are available. Software solutions such as Illumina Connected Analytics and Correlation Engine offer capabilities to analyze microarray and NGS data. Partnered solutions from Partek also offer the ability to analyze NGS, microarray, and qPCR data.

/ Results

Speak with a specialist

Reach out for information about our products and services, or get answers to questions about our technology.