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Fully customizable methylation microarray for large, targeted epigenetic applications.
Number of samples
The Infinium Custom Methylation Kit contains the Infinium HTS iSelect Methyl Custom BeadChip plus reagents to enable researchers and commercial users to develop groundbreaking assays for critical epigenetic research applications.
The Infinium HTS iSelect Methyl Custom BeadChip is offered in a 24-sample format with updated Infinium automation protocols and an automated workflow for bisulfite conversion. These features improve workflow efficiency, decrease sample processing costs, and allow users to scale their operations up to hundreds of thousands of samples per year.
The Infinium HTS iSelect Methyl Custom BeadChip is offered in a 24-sample format with updated Infinium automation protocols and an automated workflow for bisulfite conversion. These features improve workflow efficiency, decrease sample processing costs, and allow users to scale their operations up to hundreds of thousands of samples per year.
The Infinium HTS iSelect Methyl Custom BeadChip is offered in a 24-sample format with updated Infinium automation protocols and an automated workflow for bisulfite conversion. These features improve workflow efficiency, decrease sample processing costs, and allow users to scale their operations up to hundreds of thousands of samples per year.
Automation capability | Automated array loader |
---|---|
Input quantity | 250 ng DNA |
Instruments | iScan System |
Method | Methylation array |
Nucleic acid type | DNA |
Number of samples | 24 samples per array |
Specialized sample types | Blood, Cell-free DNA, Saliva |
Species category | Mouse, Human |
Technology | Microarray |
Variant class | Differentially methylated cytosines |
Requires purchase of the Infinium HTS iSelect Methyl Custom BeadChip-24 kit. Each kit contains 2 BeadChips, along with reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 48 DNA samples.
Requires an iScan Sequencing System.
Optional introductory course for 3 days of hands-on instruction covering all essential steps in the Infinium protocol, including sample and BeadChip preparation, sample scanning, and primary data evaluation and analysis using array analysis software is available for purchase.
The Infinium Custom Methylation Kit allows users to create custom high-throughput assays to measure DNA methylation in targeted regions of the genome for commercial and academic epigenetic applications, including common disease research, cancer research, epigenetic aging, and nutriepigenomics.
Methylation arrays enable high-throughput, quantitative interrogation of methylation sites across the genome at single-nucleotide resolution.
The iScan microarray scanner supports high-throughput BeadChip processing, scanning hundreds to thousands of samples quickly and accurately.
Large-scale genotyping with microarrays can identify variants associated with disease risk in large cohorts or populations.
Library Prep & Array Kit Selector
Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.
Identify sequencing panels or microarrays that target your gene(s) of interest.
DesignStudio Custom Assay Design Tool
DesignStudio is a web-based assay design tool to help researchers design and order custom genotyping microarray probes or custom sequencing assays.
Illumina methylation BeadChips employ both Infinium I and Infinium II assays, enhancing the overall breadth of coverage. (A) Infinium I probe designs use 2 bead types per CpG locus, 1 each for the methylated and unmethylated states. (B) Infinium II probe designs use 1 bead type for both states.
Researchers work with Illumina technical design and customer service teams to refine the BeadChip marker content and coordinate the ordering and production process.
Infinium Methyl Custom-24 Kit (48 Spl)
20042146
Infinium HTS iSelect Methyl Custom Beadchip, 48 samples
Infinium® Assay: An Introduction – Customer Site
20015273
Three-day, hands-on instruction at customer site to familiarize users with the essential steps in the Infinium protocol. Course provides hands-on training in sample and BeadChip preparation, sample scanning using the iScan System, and primary data evaluation and analysis using array analysis software.
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The GenomeStudio Methylation Module extracts beta values and provides sample clustering analysis for Infinium HTS iSelect Methyl Custom BeadChip data. The BeadArray Controls Reporter software tool is not supported at this time.
Notably, there are many freeware applications in Bioconductor, such as SeSAMe, that provide enhanced normalization and visualization options. Illumina does not support these freeware solutions directly.
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