Comprehensive gene fusion detection covering 507 genes from FFPE and other cancer research samples.
Assay time
Hands-on time
Input quantity
The TruSight RNA Fusion Panel covers 507 fusion-associated genes related to cancer in a single NGS assay for research use, providing results from RNA in four days.
The TruSight RNA Fusion Panel covers hundreds of genes in a single assay, increasing the possibility of fusion detection in the first round of testing.
A simple integrated workflow includes library prep, sequencing, and data analysis and can be scaled according to the number of samples. RNA samples can be multiplexed and sequenced on a benchtop system to maximize lab budgets.
On-instrument fusion calling provides NGS-based fusion detection to labs without additional bioinformatics support. The analysis provides a list of detected fusions, relevant disease associations (as identified by the Mitelman Database), and evidence of fusion-supporting reads.
| Assay time | 2.5 days |
|---|---|
| Automation details | Explore available automation methods |
| Cancer type | Pan-cancer, Hematologic, Solid tumor |
| Description | Provides a cost-effective NGS solution for cancer research, offering deep coverage of 507 genes implicated in multiple cancer types, including solid tumors, sarcomas, and hematological malignancies. |
| Hands-on time | 11 hr |
| Input quantity | 10 ng total RNA, 20–100 ng FFPE RNA |
| Instruments | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System |
| Method | Targeted RNA sequencing, Target enrichment |
| Nucleic acid type | RNA |
| Specialized sample types | Low-input samples, FFPE tissue |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Gene fusions |
Requires purchase of both Set A and Set B kitted reagents, each containing 12 unique indexes.
TruSight RNA Fusion Panel enables gene fusion detection studies with known and novel partners in 507 fusion-associated genes in multiple cancer types using blood, bone marrow, or FFPE samples.
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| MiniSeq System | Samples per run: mid output: 2-3, high output: 8 (based on 3 million reads per sample) |
2 × 76 bp (max recommended) |
| MiSeq System | 8 samples per run with v3 reagents (based on 3 million reads per sample) |
2 × 76 bp (max recommended) |
| NextSeq 550 System | 24 samples per run (mid output; based on 3 million reads per sample) |
2 × 76 bp (max recommended) |
Low-quality/FFPE RNA-Seq solutions
Find FFPE RNA sequencing solutions that yield high-quality results from degraded FFPE tissues and other low-quality samples.
Detect cancer gene expression and transcriptome changes and identify novel cancer transcripts with RNA next-generation sequencing (RNA-Seq).
| TruSight RNA Fusion Panel | TruSight Tumor 170 | AmpliSeq for Illumina Focus Panel | AmpliSeq for Illumina Comprehensive Panel v3 | |
|---|---|---|---|---|
| Assay time | 2.5 days | ~2 days | 5-6 hr (library prep only; doesn't include library quantification, normalization, or pooling time) | 5-6 hr (library prep only; doesn't include library quantification, normalization, or pooling time) |
| Automation details | Explore available automation methods | Explore available automation methods | ||
| Cancer type | Pan-cancer, Hematologic, Solid tumor | Solid tumor | Solid tumor | Solid tumor |
| Description | Provides a cost-effective NGS solution for cancer research, offering deep coverage of 507 genes implicated in multiple cancer types, including solid tumors, sarcomas, and hematological malignancies. | Perform comprehensive somatic variant detection research in solid tumors using variant calling information from both DNA and RNA. | Somatic analysis research on 52 genes associated with solid tumor cancers. | Somatic analysis research on hotspot and full-length targets of genes* associated with solid tumor cancers. |
| Hands-on time | 11 hr | ~10.5 hr | < 1.5 hr | <1.5 hr |
| Input quantity | 10 ng total RNA, 20–100 ng FFPE RNA | 40 ng DNA and/or RNA | 1–100 ng (10 ng recommended per pool) | 1–100 ng (10 ng recommended per pool) |
| Instruments | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System | NextSeq 550 System, NextSeq 500 System, HiSeq 2500 System | MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System | NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NextSeq 550Dx in Research Mode |
| Method | Targeted RNA sequencing, Target enrichment | Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment | Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing | Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing |
| Nucleic acid type | RNA | DNA, RNA | DNA, RNA | DNA, RNA |
| Specialized sample types | Low-input samples, FFPE tissue | Low-input samples, FFPE tissue | FFPE tissue | FFPE tissue |
| Species category | Human | Human | Human | Human |
| Technology | Sequencing | Sequencing | Sequencing | Sequencing |
| Variant class | Gene fusions | Gene fusions, Somatic variants, Structural variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) | Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels), Copy number variants (CNVs) | Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels), Copy number variants (CNVs) |
Library Prep and Array Kit Selector
Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.
The TruSight RNA Fusion Panel provides a simple, streamlined method for isolating targeted regions of interest from total RNA.
The Trusight RNA Fusion Panel is optimized for high performance from both high- and low-quality RNA sample types. Library prep requires as little as 10 ng total RNA, or 20 ng FFPE RNA. This low input requirement makes this panel ideal for reliable targeted analysis of limited quantity samples.
TruSight RNA Fusion Panel Set A (48 samples)
RS-304-1002
Kitted reagents for RNA library prep and enrichment of 48 total RNA samples (including FFPE) for sequencing. Each kit contains 12 unique indexes; 2, 4, 5, 6, 7, 12, 13, 14, 15, 16, 18, and 19.
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TruSight RNA Fusion Panel Set B (48 samples)
RS-304-1003
Kitted reagents for RNA library prep and enrichment of 48 total RNA samples (including FFPE) for sequencing. Each kit contains 12 unique indexes; 1, 3, 8, 9,10, 11, 20, 21, 22, 23, 25, and 27.
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Trusight RNA Fusion Oligo Panel
20046101
Enrichment oligos only. Library prep and index adapter reagents need to be ordered separately
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A high-confidence fusion call means that a fusion meets the threshold filters based on scores from calculated values of split read scores, paired read scores, break-end homology, and other features.
A low- confidence fusion call means that a fusion is listed as recurrent in the Mitelman database but does not pass or meet the minimum threshold score.
Learn more in Local Run Manager RNA Fusion Analysis Module Workflow Guide.
The Fusion Score reflects the confidence in the fusion call, where 0 is low and 1 is high. Scores > 0.6 are reported as high- confidence fusion calls.
This assay is being automated by some of our automation providers. If you are interested in automating this workflow, contact your account manager.
Chromosomal translocations resulting in overexpression or deletion of a transcript can be reflected in gene expression levels but would not create a fusion gene. The Local Run Manager RNA Fusion module is not designed for detection of gene expression changes. To detect these changes, the RNA-Seq Alignment App in BaseSpace Sequence Hub is recommended. In addition, it is recommended to confirm these findings in DNA.
Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
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