AmpliSeq for Illumina Transcriptome Human Gene Expression Panel
Targeted panel that measures expression levels of >20,000 human RefSeq genes.Read More...
Panel
Library Prep
Index Adapters
The AmpliSeq for Illumina Transcriptome Human Gene Expression Panel is a highly multiplexed targeted resequencing assay for measuring expression levels of over 20,000 human RefSeq genes in a single assay. Key features include:
Prepare libraries in 6 hours with < 1.5 hours hands-on time; results available in < 2 days.
Obtain high-quality data even when starting with minimal input from formalin-fixed, paraffin-embedded (FFPE) tissues.
Take advantage of a comprehensive workflow that spans library preparation, sequencing, and analysis.
The Transcriptome Human Gene Expression Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
| AmpliSeq for Illumina Transcriptome Human Gene Expression Panel | Illumina Stranded Total RNA Prep with Ribo-Zero Plus | TruSeq Stranded Total RNA | TruSeq Stranded Total RNA with Ribo-Zero Globin | |
|---|---|---|---|---|
| Content Specifications | > 95% of human RefSeq genes; 20,802 genes; captures human transcriptome | 捕获编码RNA以及多种形式的非编码RNA。 | 捕获编码RNA以及多种形式的非编码RNA。 | 捕获编码RNA以及多种形式的非编码RNA。 |
| Description | Study the expression levels of >20,000 human RefSeq genes using sequence-specific amplicons. | 用于多种类型样本的全转录组测序研究。包含Ribo-Zero plus模块，一个试管即可去除多个物种的转录本。 | For whole-transcriptome sequencing studies. The Total RNA version depletes cytoplasmic rRNA while the Total RNA Gold version depletes cytoplasmic and mitochondrial rRNA. | 用于血液来源RNA的全转录组测序研究。去除细胞质rRNA、线粒体rRNA和珠蛋白mRNA。 |
| Input Quantity | 1-100 ng RNA (10 ng recommended) | 对于标准质量RNA样本，可使用1–1000 ng的总RNA。对于FFPE样本，建议最少使用10 ng的总RNA，以获得理想效果。 | 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization. | 0.1–1 ug血液来源的高质量纯化总RNA |
| Method | Exome Sequencing , mRNA Sequencing , 靶向RNA测序 | 全转录组测序 | 全转录组测序 | 全转录组测序 |
| Specialized Sample Types | Blood, FFPE Tissue | FFPE Tissue, Low-Input Samples | FFPE Tissue | Blood |
| Species Category | 人类 | Virus, 人类, 大鼠, 小鼠, 细菌 | 人类, 大鼠, 小鼠 | 人类, 大鼠, 小鼠 |
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
Sample Identification with the AmpliSeq for Illumina Sample ID Panel Application Note
Application Note | HTML
Data Sheet | HTML | 5 versions
AmpliSeq for Illumina Transcriptome Human Gene Expression Panel Gene List
product_file | EXCEL< 1 MB
AmpliSeq for Illumina Transcriptome Human Gene Expression Panel Reference Guide Documentation
Illumina Adapter Sequences Documentation
Index Adapters Pooling Guide Documentation
AmpliSeq for Illumina Transcriptome Human Gene Expression Panel Checklist Documentation
Illumina Transcriptome Human Gene Expression Panel Support - Documentation & Literature
自定义协议选择器
Generates customized, end-to-end instructions
全部 AmpliSeq for Illumina Transcriptome Human Gene Expression Panel Support
Targeted DNA and RNA research panel investigating variants across 161 genes associated with a range of cancer types.
Rapidly prepare amplicon libraries for Illumina sequencers using AmpliSeq for Illumina library preparation and index adapters.
