A streamlined workflow offering highly sensitive detection and characterization of common respiratory viruses, including SARS-CoV-2 strains.
Assay time
Hands-on time
Input quantity
The Respiratory Virus Oligo Panel v2 (RVOP v2, Catalog no. 20044311) is now sold in comprehensive kits (Sets A-D) that include the Respiratory Virus Oligo Panel V2, library prep and enrichment reagents, and index adapters. The standalone RVOP v2 product or individual components can be purchased through MyIllumina.
The Illumina Respiratory Virus Enrichment Kit, formerly known as the Respiratory Virus Oligo Panel (RVOP), provides an effective, streamlined method for detecting common respiratory viruses, such as SARS-CoV-2 and influenza A/B.
Target enrichment through the hybrid–capture method allows for highly sensitive detection without requiring the high read depth needed for shotgun metagenomic sequencing. Additionally, this method allows for dramatically larger probe panels for comprehensive profiling compared to amplicon sequencing while producing whole-genome sequencing data for the targeted viruses.
This comprehensive workflow integrates library preparation, target enrichment, sequencing, and data analysis, giving researchers and public health scientists the ability to monitor multiple viruses and variants with one workflow
| Assay time | < 9 hr library prep time |
|---|---|
| Automation capability | Liquid Handling Robots |
| Automation details | Explore available automation methods |
| Content specifications | Targets and characterizes ~40 common respiratory viruses, including SARS-CoV-2, influenza A and B viruses, adenovirus, rhinovirus, respiratory syncytial virus, and other common respiratory viruses. |
| Description | A streamlined workflow for detecting and analyzing SARS-CoV-2 and other common respiratory viruses. The agnostic design allows for widespread identification of pathogenic viruses across multiple sample types of interest. |
| Hands-on time | < 2 hr library prep time |
| Input quantity | 10-100 ng |
| Instruments | MiSeq System, NextSeq 550 System, NextSeq 2000 System, MiniSeq System |
| Mechanism of action | On-bead tagmentation followed by a single hybridization step |
| Method | Target Enrichment, Targeted RNA Sequencing, Targeted DNA Sequencing |
| Multiplexing | Up to 384 samples in a single run with unique dual indexes |
| Nucleic acid type | DNA, RNA |
| Sample type details |
Nasal swabs and washes, wastewater, and more |
| Species category | Human, Virus |
| Species details | Detects common respiratory viruses, including recent influenza virus strains and SARS-CoV-2. The panel includes probes to human targets as a quality feature, and therefore detects those human targets in every sample assayed. |
| Technology | Sequencing |
| Variant class | Single nucleotide polymorphisms (SNPs), Single nucleotide variants (SNVs), Insertions-deletions (indels) |
Illumina Respiratory Virus Enrichment Kit, Illumina sequencing, and DRAGEN secondary analysis offer a streamlined workflow that enables the detection and characterization of over 40 common human respiratory viruses.
llumina Respiratory Virus Enrichment Kit
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| MiniSeq System | Rapid: 20 samples, 1M clusters and a minimum of 1M single-end reads per sample | 1 × 100 bp |
| MiSeq System | v3 reagents: ~20 samples, 1M clusters and a minimum of 1 M single-end reads per sample | 1 × 150 bp |
| NextSeq 550 System | High output: 384 samples, 1M clusters and a minimum of 1M single-end reads per sample | 1 × 150 bp |
Target enrichment captures genomic regions of interest through hybridization. Learn more about target enrichment and how it can be used to assess multiple samples at once.
NGS can identify novel coronavirus variants, track COVID-19 transmission, and more. Compare NGS methods for various coronavirus sequencing goals.
As a hypothesis-free method, NGS can distinguish between infectious disease strains that differ by as little as one SNP, and replace multiple tests.
| Illumina Respiratory Virus Enrichment Kit | Respiratory Pathogen ID/AMR Enrichment Panel Kit | |
|---|---|---|
| Assay time | < 9 hr library prep time | < 9 hr library prep time |
| Automation capability | Liquid Handling Robots | Liquid Handling Robots |
| Automation details | Explore available automation methods | Explore available automation methods |
| Content specifications | Targets and characterizes ~40 common respiratory viruses, including SARS-CoV-2, influenza A and B viruses, adenovirus, rhinovirus, respiratory syncytial virus, and other common respiratory viruses. | Detects respiratory pathogen DNA and RNA simultaneously and profiles antimicrobial resistance (AMR) gene expression concurrently. |
| Description | A streamlined workflow for detecting and analyzing SARS-CoV-2 and other common respiratory viruses. The agnostic design allows for widespread identification of pathogenic viruses across multiple sample types of interest. | Identify respiratory infections and co-infections, detect antimicrobial resistance markers, and perform strain typing of critical pathogens (SARS-CoV-2 and Flu A/B viruses) to study viral evolution and transmission. |
| Hands-on time | < 2 hr library prep time | < 2 hr library prep time |
| Input quantity | 10-100 ng | Based on volume (not concentration dependent) |
| Instruments | MiSeq System, NextSeq 550 System, NextSeq 2000 System, MiniSeq System | MiSeq System, NextSeq 550 System, MiniSeq System |
| Mechanism of action | On-bead tagmentation followed by a single hybridization step | On-bead tagmentation followed by a single hybridization step |
| Method | Target Enrichment, Targeted RNA Sequencing, Targeted DNA Sequencing | Target Enrichment, Targeted RNA Sequencing, Targeted DNA Sequencing |
| Multiplexing | Up to 384 samples in a single run with unique dual indexes | Up to 384 samples in a single run with unique dual indexes |
| Nucleic acid type | DNA, RNA | DNA, RNA |
| Sample type details |
Nasal swabs and washes, wastewater, and more |
|
| Species category | Human, Virus | Fungal, Virus, Bacteria |
| Species details | Detects common respiratory viruses, including recent influenza virus strains and SARS-CoV-2. The panel includes probes to human targets as a quality feature, and therefore detects those human targets in every sample assayed. | Detects respiratory pathogens (180+ bacteria, 50+ fungi, and 40+ viruses, including SARS-CoV-2) and antimicrobial resistance alleles (1200+). |
| Technology | Sequencing | Sequencing |
| Variant class | Single nucleotide polymorphisms (SNPs), Single nucleotide variants (SNVs), Insertions-deletions (indels) |
Library Prep and Array Kit Selector
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The streamlined NGS workflow for respiratory virus detection integrates sample preparation, library preparation, target enrichment, sequencing, and data analysis.
In a titration experiment of human saliva RNA spiked with a defined amount of virus copies, the Illumina Respiratory Virus Enrichment Kit resulted in detection of viral reads from as low as two copies of SARS-CoV-2 viral RNA.
The Respiratory Virus Oligo Panel includes 7800 probes to detect common respiratory viruses, recent flu strains, SARS-CoV-2, and human probes to act as positive controls. All probes are 80-mer oligos, spaced very close together, providing full genome coverage of all viruses in the panel.
| Human coronavirus 229E | Human parainfluenza virus 1 | Human bocavirus 4 NI strain HBoV4-NI-385 | Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)) |
| Human coronavirus NL63 | Human parainfluenza virus 2 | KI polyomavirus Stockholm 60 | Influenza A virus (A/Hong Kong/1073/99(H9N2)) |
| Human coronavirus OC43 | Human parainfluenza virus 3 | WU Polyomavirus | Influenza A virus (A/Texas/50/2012(H3N2)) |
| Human coronavirus HKU1 | Human parainfluenza virus 4a | Human parechovirus type 1 PicoBank/HPeV1/a | Influenza A virus (A/Michigan/45/2015(H1N1)) |
| SARS-CoV-2 | Human metapneumovirus (CAN97-83) | Human parechovirus 6 | Influenza B virus (B/Lee/1940) |
| Human adenovirus B1 | Respiratory syncytial virus (type A) | Human rhinovirus A89 | Influenza B virus (B/Wisconsin/01/2010) |
| Human adenovirus C2 | Human Respiratory syncytial virus 9320 (type B) | Human rhinovirus C (strain 024) | Influenza B virus (B/Brisbane/60/2008) |
| Human adenovirus E4 | Influenza A virus (A/Puerto Rico/8/1934(H1N1)) | Human rhinovirus B14 | Influenza B virus (B/Colorado/06/2017) |
| Human bocavirus 1 (Primate bocaparvovirus 1 isolate st2) | Influenza A virus (A/Korea/426/1968(H2N2)) | Human enterovirus C104 strain: AK11 | Influenza B virus (B/Washington/02/2019) |
| Human bocavirus 2c PK isolate PK-5510 | Influenza A virus (A/New York/392/2004(H3N2)) | Human enterovirus C109 isolate NICA08-4327 | Human control genes |
| Human bocavirus 3 | Influenza A virus (A/goose/Guangdong/1/1996(H5N1)) |
Illlumina Respiratory Virus Enrichment Kit Set A (96 indexes, 96 samples)
20100469
Complete hybridization-based enrichment kit including Illumina RNA Prep with Enrichment library preparation and hybridization reagents, Respiratory Virus Oligo Panel v2, and IDT® for Illumina® DNA/RNA UD Indexes Set A. Each kit provides enough reagents for 96 samples in 32 hybridization reactions with 3 libraries per hybridization reaction. Enables enrichment of 40 common human respiratory viruses including SARS-CoV-2 and Influenza and produces whole genome sequences for each targeted virus.
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Illumina Respiratory Virus Enrichment Kit Set B (96 indexes, 96 samples)
20100470
Complete hybridization-based enrichment kit including Illumina RNA Prep with Enrichment library preparation and hybridization reagents, Respiratory Virus Oligo Panel v2, and IDT® for Illumina® DNA/RNA UD Indexes Set B. Each kit provides enough reagents for 96 samples in 32 hybridization reactions with 3 libraries per hybridization reaction. Enables enrichment of 40 common human respiratory viruses including SARS-CoV-2 and Influenza and produces whole genome sequences for each targeted virus.
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Illumina Respiratory Virus Enrichment Kit Set C (96 indexes, 96 samples)
20100471
Complete hybridization-based enrichment kit including Illumina RNA Prep with Enrichment library preparation and hybridization reagents, Respiratory Virus Oligo Panel v2, and IDT® for Illumina® DNA/RNA UD Indexes Set C. Each kit provides enough reagents for 96 samples in 32 hybridization reactions with 3 libraries per hybridization reaction. Enables enrichment of 40 common human respiratory viruses including SARS-CoV-2 and Influenza and produces whole genome sequences for each targeted virus.
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Discounts:
Illumina Respiratory Virus Enrichment Kit Set D (96 indexes, 96 samples)
20100472
Complete hybridization-based enrichment kit including Illumina RNA Prep with Enrichment library preparation and hybridization reagents, Respiratory Virus Oligo Panel v2, and IDT® for Illumina® DNA/RNA UD Indexes Set D. Each kit provides enough reagents for 96 samples in 32 hybridization reactions with 3 libraries per hybridization reaction. Enables enrichment of 40 common human respiratory viruses including SARS-CoV-2 and Influenza and produces whole genome sequences for each targeted virus.
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The Illumina Respiratory Virus Enrichment Kit includes free usage of the DRAGEN RNA Pathogen Detection app and the DRAGEN Targeted Microbial app, both of which analyze pathogen data and generate consensus sequences.
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