BaseSpace™ Sequencing Hub: FASTQ Processing Tools for Data Analysis

FASTQ files store biological sequence and quality information and are key for downstream data analysis pipelines. Several steps are necessary during FASTQ generation to ensure optimal data analysis. This webinar is targeted at new and intermediate users including biologists intending to understand output of basic bioinformatics tools used for processing FASTQs.

We will focus on BaseSpace Sequence Hub tools such as FASTQC and FASTQ toolkit. We will cover the following topics: What is a FASTQ file; An overview of Illumina FASTQ generation tools; FASTQ processing such as adapter trimming, masking, quality trimming, etc; and Highlight the differences between Illumina run performance metrics and output generated by third party tools such as FASTQC.

Note: This webinar is intended for users who are new to Illumina sequencing platform and end users who use core facility to sequence their data and need some basic guidance on data optimization. This webinar is not intended for bioinformaticians.

 

Speakers

Wei Wen Lim
Technical Application Scientist
Illumina

Wei Wen graduated with a Bachelor’s degree with Honours in Biochemistry and Immunology from Monash University in Australia. She previously worked in an oncology research environment, where she gained hands on experience on NGS. Wei Wen joined Illumina in 2023 as a Technical Applications Scientist. She is passionate about applying her knowledge and experience in troubleshooting technical challenges. She is now specialized in supporting customers on Illumina instrumentation, microarray and clinical workflows.