Targeted Resequencing: When to Choose Amplicon or Enrichment

July 30, 2021

Learn about considerations for choosing an amplicon or enrichment approach to targeted resequencing. Discussions about each workflow, benefits of each, and some considerations for choosing an amplicon or enrichment approach to your targeted resequencing experiments. Presenter: Matt Angel – Executive Sequencing Specialist, Illumina More Resources: Explore Sequencing Methods: illumina.com/science/sequencing-method-explorer.html
Support Webinars: *Amplicon Sequencing: Assessing the success of your run youtu.be/ENAlPYK1_X0 *Amplicon Sequencing: Introduction youtu.be/Gj1fGbVGPn0 *Illumina DNA Prep with Enrichment (formerly Nextera DNA Flex for Enrichment): Introduction youtu.be/x92G5w96in4 *Sequencing: How to Plan Your First Sequencing Project youtu.be/1ykx_AwtMJI Support Bulletins: *Library quantification and quality control quick reference guide support.illumina.com/bulletins/2016/05/library-quantification-and-quality-control-quick-reference-guide.html
*Bead types in Illumina library preparation kits support.illumina.com/bulletins/2020/07/bead-types-in-illumina-library-preparation-kits.html
*Understanding library pooling for Illumina DNA enrichment kits support.illumina.com/bulletins/2017/09/understanding-library-pooling-for-illumina-dna-enrichment-kits.html
*Illumina library prep kits and associated index kits (bulletin) support.illumina.com/bulletins/2020/06/illumina-library-prep-kits-and-associated-index-kits.html
00:00 Introduction to Bite-sized learning series. 00:50 Why next generation sequencing over other methods & Basics of next generation sequencing. 02:13 What is targeted resequencing and why is it important. 05:35 Amplicon & Enrichment Workflows 07:36 Which method is best for my research goals? 11:44 Considerations for amplicon vs. enrichment methods. 14:19 Targeted Resequencing Resources Subscribe to the Illumina video channel youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. *Data calculations on file. Illumina, Inc., 2015. View customer spotlight videos youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina product videos youtube.com/playlist?list=PLKRu7cmBQlaj6YuZmkfxZcT9twqDgP2Xd View Illumina support videos youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM
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