NextSeq 1000/2000 Reagents

Reagents for the NextSeq 1000/2000 System feature easy-to-use cartridges and multiple flow cell configurations for flexible sequencing options.

In 1H 2024, P4 shelf life will be 3 months. This will be extended to 6 months in 2H of 2024.

Overview

NextSeq 1000/2000 reagents leverage XLEAP-SBS chemistry to enable a wide range of applications.

These reagents offer:

  • Sequencing output of 10–540 Gb per run, providing adjustable output based on project needs

  • Multiple flow cell configurations that offer flexibility to support new and emerging applications

Sustainability and simplicity in one

Easy-to-use reagent cartridges deliver a streamlined workflow that reduces the chance for errors, lowers operating costs, improves recyclability, and minimizes waste. The fully-integrated cartridges include reagents, fluidics, and the waste holder, streamlining library loading and instrument use. 

Simply prepare the reagent cartridge, insert the flow cell, load the library into the cartridge, and insert into the instrument. 


Required products

Use XLEAP-SBS P1 reagents on both the NextSeq 1000 and NextSeq 2000 Systems. Maximum output: 60 Gb. Maximum single-end reads per run: 100M.

Use XLEAP-SBS P2 reagents on both the NextSeq 1000 and NextSeq 2000 Systems. Maximum output: 240 Gb. Maximum single-end reads per run: 400M. 

Use XLEAP-SBS P3 reagents on the NextSeq 2000 System only. Maximum output: 360 Gb. Maximum single-end reads per run: 1.2B.

Use XLEAP-SBS P4 reagents on the NextSeq 2000 System only. Maximum output: 540 Gb. Maximum single-end reads per run: 1.8B.

The NextSeq 1000/2000 Index Primer Kit, Read Primer Kit, and Read and Index Primers are optional but required when both custom sequencing primers and Illumina primers are used in a run.* 

PhiX Control v3 is recommended for all runs, but is optional for well-balanced library types. 

/ Results

*The XLEAP-SBS cartridge already contains the primers for Illumina DNA PCR-Free Prep, whereas the standard SBS cartridge does not.

Documentation

Product literature

XLEAP-SBS reagents (17)

NextSeq™ 1000/2000 P1 XLEAP-SBS™ Reagent Kit (600 Cycles)

20100981

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 600 cycles of sequencing, plus dual-indexing support (up to 100M single reads).

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NextSeq™ 1000/2000 P1 XLEAP-SBS™ Reagent Kit (300 Cycles)

20100982

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 100M single reads).

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NextSeq™ 1000/2000 P1 XLEAP-SBS™ Reagent Kit (100 Cycles)

20100983

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 100M single reads).

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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (600 Cycles)

20100984

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 600 cycles of sequencing, plus dual-indexing support (up to 400M single reads).

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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (300 Cycles)

20100985

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 400M single reads).

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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (200 Cycles)

20100986

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 400M single reads).

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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (100 Cycles)

20100987

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 400M single reads).

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NextSeq™ 2000 P3 XLEAP-SBS™ Reagent Kit (300 Cycles)

20100988

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 1.2B single reads).

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NextSeq™ 2000 P3 XLEAP-SBS™ Reagent Kit (200 Cycles)

20100989

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 1.2B single reads).

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NextSeq™ 2000 P3 XLEAP-SBS™ Reagent Kit (100 Cycles)

20100990

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 1.2B single reads).

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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (300 Cycles)

20100992

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).

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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (200 Cycles)

20100993

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).

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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (100 Cycles)

20100994

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).

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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (50 Cycles)

20100995

Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 50 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).

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NextSeq™ 1000/2000 XLEAP-SBS™ Read and Index Primer Kit

20112856

Reagents to utilize custom read and index primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge. This kit is sufficient for 1 custom read primer and 1 custom index primer use.

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NextSeq™ 1000/2000 XLEAP-SBS™ Index Primer Kit

20112858

Reagents to utilize custom index primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge. This kit is sufficient for 10 custom index primer uses.

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NextSeq™ 1000/2000 XLEAP-SBS™ Read Primer Kit

20112859

Reagents to utilize custom read primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge. This kit is sufficient for 10 custom read primer uses.

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Standard SBS reagents (11)

NextSeq™1000/2000 P1 Reagents(100cycles)

20074933

Provides kitted reagents for 100 cycles of sequencing, plus dual- indexing support (up to 100M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (100 cycles), NextSeq 1000/2000 P1 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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NextSeq 1000/2000 P1 Reagents (300 Cycles)

20050264

Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support (up to 100M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (300 cycles), NextSeq 1000/2000 P1 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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NextSeq™1000/2000 P1 Reagents(600cycles)

20075294

Provides kitted reagents for 600 cycles of sequencing, plus dual- indexing support (up to 100M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (600 cycles), NextSeq 1000/2000 P1 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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NextSeq 1000/2000 P2 Reagents (100 Cycles) v3

20046811

Provides kitted reagents for 100 cycles of sequencing, plus dual- indexing support (up to 400M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (100 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20.

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NextSeq 1000/2000 P2 Reagents (200 Cycles) v3

20046812

Provides kitted reagents for 200 cycles of sequencing, plus dual- indexing support (up to 400M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (200 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20.

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NextSeq 1000/2000 P2 Reagents (300 Cycles) v3

20046813

Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support (up to 400M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (300 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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NextSeq 2000 P3 Reagents (50 Cycles)

20046810

Provides kitted reagents for 50 cycles of sequencing, plus dual-indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (50 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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NextSeq 2000 P3 Reagents (100 Cycles) 

20040559

Provides kitted reagents for 100 cycles of sequencing, plus dual- indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (100 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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NextSeq 2000 P3 Reagents (200 Cycles)

20040560

Provides kitted reagents for 200 cycles of sequencing, plus dual- indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (200 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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NextSeq 2000 P3 Reagents (300 Cycles)

20040561

Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (300 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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NextSeq™1000/2000 P2 300M Reagents(600cycles)

20075295

Provides kitted reagents for 600 cycles of sequencing, plus dual- indexing support (up to 300M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (600 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.

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Accessory products (5)

NextSeq 1000/2000 Read and Index Primers

20046115

Reagents to utilize custom read and index primers with the NextSeq 1000/2000 cartridge. This kit is sufficient for 1 custom read primer and 1 custom index primer use.

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NextSeq 1000/2000 Index Primer Kit

20046116

Reagents to utilize custom index primers with the NextSeq 1000/2000 cartridge. This kit is sufficient for 10 custom index primer uses.

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NextSeq 1000/2000 Read Primer Kit

20046117

Reagents to utilize custom read primers with the NextSeq 1000/2000 cartridge. This kit is sufficient for 10 custom read primer uses.

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PhiX Control v3

FC-110-3001

Kitted DNA control for the Illumina sequencing platform. Compatible with Single and Paired End reads up to 150 base pairs. (10ul of 10nM template solution)

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NextSeq 1000/2000 Recycling Tool

20050631

Tool used to break down NextSeq 1000/2000 cartridge into individual components for easy recycling

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FAQs

A variety of applications can be run on the NextSeq 2000 System. Each application will have different run times. Run times can range from 8–44 hr. 

See the NextSeq 2000 specifications for more information.

The NextSeq 2000 System can generate 1.8B single-end reads with P4 reagents.

See the NextSeq 2000 specifications for more information.

For maximum flexibility, the NextSeq 2000 System offers four flow cell types.

See the NextSeq 2000 specifications for more information.

/ Results

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