MiSeq i100 Series

Fastest, simplest. For every lab.

Remarkably simple from run setup to data analysis

The new standard in benchtop sequencing speed and simplicity

The MiSeq i100 Series delivers our fastest run times yet, breakthrough simplicity, and significant sustainability advancements to empower every lab, everywhere

Scientist starting a run on the MiSeq i100 Plus System

Incredible speed for same-day results

Same-day (and same-shift) results are now possible. The MiSeq i100 and MiSeq i100 Plus Systems provide results 4× faster than the MiSeq System, with sequencing run times as fast as four hours.

With the expanded output offered by the MiSeq i100 Series, you can increase sample throughput, perform deeper sequencing, and gain more insights for a wide range of applications faster than ever. XLEAP-SBS chemistry on the MiSeq i100 Series eliminates thawing requirements so reagents can be used whenever your sample is ready.

Loading reagents into the MiSeq i100

The MiSeq i100 Series makes NGS simpler than ever

Every aspect of the MiSeq i100 Series workflow is noticeably simplified, from setup to data analysis. Whether you are new to sequencing or an experienced user, you’ll appreciate the streamlined operations. Run setup requires only three steps and can be completed in less than 20 minutes.

Integrated cartridges simplify library loading and eliminate the need for washes between runs, improving sequencing efficiency.

Generate data analysis summaries in two hours or less for most applications by accessing preconfigured DRAGEN pipelines, either onboard or in the cloud.

Scientist removing reagent from sustainable packaging

Ground-breaking sustainability innovations

In addition to breakthrough advancements in system design, XLEAP-SBS chemistry, and integrated data analysis, the MiSeq i100 Series is optimized to reduce environmental impact. Key innovations, such as an 85% reduction in packaging waste compared to the MiSeq System and the use of recyclable plastics and room-temperature ship-and-store consumables, deliver remarkable benefits in terms of sustainability and user experience.

Scientist pipetting

Trusted technology, trusted partner

Trusted for over a decade, the MiSeq System has been shipped to over 10,000 customers globally, and has been cited in over 160,000 peer-reviewed publications.1

Building on our extensive expertise, Illumina has a relentless commitment to innovation and building future next-generation sequencing (NGS) capabilities and applications. The MiSeq i100 Series demonstrates our commitment to increasing access to genomics technology by continuing to deliver faster, simpler sequencing.

MiSeq i100 Series specificationsa

  • Output rangeb
    1.5–30 Gb
  • Paired-end reads per run
    10–200M
  • Max read length
    2 x 300 bp
  • Run time
    ~4–15.5 hr

a. Specifications based on Illumina PhiX control library at supported cluster densities.

b. Maximum range based on 100M flow cell specifications. The 100M flow cell will be available starting in 2025 for the MiSeq i100 Plus System only.

Tour the MiSeq i100 Series

See how the MiSeq i100 Series is setting the standard for simplicity, usability, and speed of benchtop sequencing from Illumina.

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Support when you need it

Illumina provides a world-class support team composed of experienced scientists who are experts in library prep, sequencing, and analysis. This exceptional technical support is available via phone five days a week or online 24/7, worldwide, and in multiple languages, with rapid response time near most major metropolitan areas.

To help you get high-quality results on Illumina technology even faster, we offer instructor-led or hands-on courses and web-based training options.

 

Sample-to-analysis NGS solutions

With both supported workflows and a large ecosystem of choices, Illumina products are available for every step of the MiSeq i100 Series workflow.

1
Prepare libraries

With the MiSeq i100 Series, libraries are loaded in three simple steps and in under 20 minutes.

2
Sequence

Increase efficiency with XLEAP-SBS chemistry and streamlined operations, including onboard denaturation and clustering to obtain fast, high-quality data.

3
Analyze data

Obtain accurate and efficient secondary analysis with preconfigured DRAGEN pipelines onboard or in the cloud, generating results in two hours or less for most applications.

4
Interpret results

Gain insights and drive discovery with cloud-based tertiary analysis solutions for select workflows.

Full solutions for varied applications

The MiSeq i100 Series offers highly accurate quality data for various applications, including oncology, infectious disease, and microbiology studies.

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Compare the MiSeq i100 Series

 

MiSeq i100 Series

MiSeq i100 Seriesa

MiSeq System

MiSeq System

NextSeq 1000 and 2000 Systems

NextSeq 1000 and 2000 Systems

Run time (range) ~4–15.5 hr ~5–56 hr 8–44 hr
Max output 30 Gb 15 Gb 540 Gb
Max reads per run

100 million single reads
200 million paired-end reads

25 million single reads
50 million paired-end reads
1.8 billion single reads
3.6 billion paired-end reads
Informatics options

On-instrument: Custom menu of DRAGEN analysis pipelines

Cloud: Broad menu of DRAGEN analysis applications available on BaseSpace Sequence Hub and Illumina Connected Analytics

On-premises: Broad menu of DRAGEN analysis applications

On-instrument: LRM analysis modules

Cloud: Broad menu of DRAGEN analysis applications available on BaseSpace Sequence Hub and Illumina Connected Analytics

On-premises: Broad menu of DRAGEN analysis applications

On-instrument: Custom menu of DRAGEN analysis pipelines

Cloud: Broad menu of DRAGEN analysis applications available on BaseSpace Sequence Hub and Illumina Connected Analytics

On-premises: Broad menu of DRAGEN analysis applications

a. Maximum range based on 100M flow cell specifications. The 100M flow cell will be available starting in 2025 for the MiSeq i100 Plus System only.

Are you new to sequencing or considering your first instrument?

These resources cover key topics in NGS designed for beginners. We'll provide tutorials, guide you through the workflow and planning your first experiment.

Illumina sequencing systems

Financing options

Get up and running quickly with a rental or leasing program designed to work with your budget. Choose from several options to obtain financing, including instrument leasing, instrument rental, and reagent rental.

Illumina Proactive service

Illumina Proactive is a secure and remote instrument performance and support service. Our field support team can identify risks before your run, perform reagent lot tracking, maximize uptime, and improve lab efficiency. Register for a MyIllumina account to get started.

Popular products

MiSeq i100 Series Reagent Kits

Reagents for the MiSeq i100 Series feature easy-to-use cartridges and multiple flow cell configurations for flexible sequencing options.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

Illumina RNA Prep with Enrichment

A fast, integrated workflow for producing enriched and indexed sequencing libraries from a broad range of sample types and RNA input quantities.

Illumina Microbial Amplicon Prep

A flexible and streamlined NGS library prep kit that enables various public health surveillance and microbial research applications.

Speak to a specialist or attend an event

Looking for more? Reach out to talk to a specialist about the MiSeq i100 Series or explore a variety of events introducing the MiSeq i100 Series, from roadshows in cities around the world to webinars and more.

References
  1. Illumina Internal Data on File, 2023.