MiSeq i100 Series applications and methods

Simplest workflow, fastest results

Perform a wide range of applications on our fastest, simplest benchtop sequencer, with flexible output and read-to-use workflows optimized for novice users

Scientist starting a sequencing workflow on MiSeq i100 System

Featured application resources

Benchtop applications ebook

Download this eBook to learn how benchtop sequencers support a broad range of applications while delivering exceptional data quality and accuracy.

NGS workflow finder

Use the NGS Workflow Finder to get recommendations for your application workflows of interest so you can sequence with confidence.

NGS library prep

Watch this webinar unveiling the MiSeq i100 Series. View application data in oncology, microbiology, and infectious disease, and gain insights from early-access users.

Key applications and methods

Whether you use our predesigned supported workflows or build your own, we have options to support every step of the workflow.

Supported sample-to-analysis workflows: The MiSeq i100 and MiSeq i100 Plus Systems offer supported sample-to-analysis workflows for various methods. These workflows include library prep kits, panels, sequencing on the MiSeq i100 Series, DRAGEN secondary analysis, and data interpretation (where applicable). Additional workflows will be supported in the future.

Microbiome and pathogen discovery

Targeted, culture-free sequencing of complex biological samples to identify and compare bacterial taxa present in microbial communities.

Supported sample-to-analysis workflow

16S amplicon sequencing

Culture-free NGS method that identifies microbes in complex samples. A cost-effective, culture-free method for identification of bacterial strains not found using traditional methods.

Single-genome microbial sequencing

Microbial sequencing and analysis of a single, known organism, whether from an isolated, cultured bacterial colony or a primary sample being surveilled for a determined pathogen of interest.

Supported sample-to-analysis workflow

Microbial WGS

Comprehensive method for mapping, finishing, or comparing microbial genomes for deep insights. Fast, integrated library prep completes in < 4 hr with minimal hands-on time.

1
Library prep
3
Data analysis & interpretation

DRAGEN sWGS onboard MiSeq i100

Resources

Multipathogen sequencing

Multipathogen sequencing via amplicon-based NGS involves analyzing genomic regions of interest with ultradeep sequencing of PCR amplicons. Enables full characterization of viruses.

Supported sample-to-analysis workflow

Viral public health threat sequencing

Obtain WGS data for characterization of a broad range of viruses, including SARS-CoV-2, influenza, and poliovirus. Perform concurrent analysis of RNA and DNA viruses compatible with a range of sample types.

1
Library prep
3
Data analysis & interpretation

DRAGEN Microbial Enrichment Plus onboard MiSeq i100

Common respiratory virus sequencing

Targeted, streamlined detection of common respiratory viruses, such as SARS-CoV-2 and influenza A/B. Target enrichment through hybrid–capture methods provides highly sensitive detection without the need for high read depth.

1
Library prep
3
Data analysis & interpretation

DRAGEN Microbial Enrichment Plus onboard MiSeq i100

Targeted cancer sequencing

Targeted cancer sequencing, comprising a focused selection of genes associated with specific cancer types or pathways, complement broader genomic profiling approaches and offer users multiple options to interrogate and understand cancer biology.

Supported sample-to-analysis workflow

Multicancer CNV and fusion analysis

Targeted detection of multiple variants, including CNVs and gene fusions. Single-tube SLIMamp workflow provides fast library prep and turnaround time.

1
Library prep

Library prep

SLIMamp Amplicon Prep

3
Data analysis & interpretation

Data analysis

Amplicon Pillar PiVAT

Supported sample-to-analysis workflow

Myeloid cancer gene detection

Targeted interrogation of 58 genes of interest highly relevant to myeloid cancers. Single-tube SLIMamp workflow provides fast library prep and turnaround time.

1
Library prep

Library prep

SLIMamp Amplicon Prep

3
Data analysis & interpretation

Data analysis

DRAGEN DNA

Amplicon Pillar PiVAT

Supported sample-to-analysis workflow

Solid tumor fusion detection

Focused detection of multiple gene rearrangement regions of interest across solid tumor types. Single-tube SLIMamp workflow provides fast library prep and turnaround time.

1
Library prep

Panel

Pillar oncoReveal Multi-Cancer RNA Fusion v2 Panel

Library prep

SLIMamp Amplicon Prep

3
Data analysis & interpretation

Data analysis

DRAGEN DNA

Amplicon Pillar PiVAT

Cancer gene expression and fusion detection

Comprehensive assessment of 1385 cancer-related RNA transcripts. In-depth and cost-effective assessment of cancer-related transcripts across most sample types, including FFPE samples.

1
Library prep

Library prep

SLIMamp Amplicon Prep

3
Data analysis & interpretation

Data analysis

Amplicon Pillar PiVAT

Related content

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MiSeq i100 Series: Introduction to starting a run

This video walks you step-by-step through planning your run, preparing your samples for sequencing, and setting up the instrument.

NGS library preparation

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XLEAP-SBS chemistry

Learn about our fastest, most robust, highest-quality sequencing chemistry to date.

Speak to a specialist

Looking for more? Reach out to talk to a specialist about the MiSeq i100 Series.