Simplified operations, advanced chemistry, and onboard informatics provide an easy-to-use, fast, and flexible instrument supporting a broad range of applications.
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In this video, an Illumina Field Applications Scientist discusses what to expect during the preinstallation, shipping, and delivery stages. We’ll highlight important steps in the process and introduce you to available resources.
MiSeq i100 Series training
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MiSeq i100 Plus System
20115695
The MiSeq i100 Plus System is a benchtop sequencing system that integrates sequencing, primary analysis and secondary analysis. The system includes embedded touchscreen monitor and on-instrument computer, control software, hardware-accelerated DRAGEN secondary analysis pipelines, and 12 months warranty (including parts and labor). The system is self-installable by the customer and does not include installation services.
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NextSeq™ 1000/2000 XLEAP-SBS™ Read and Index Primer Kit
20112856
Reagents to utilize custom read and index primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge and MiSeq i100 series cartridges. This kit is sufficient for 1 custom read primer and 1 custom index primer use
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NextSeq™ 1000/2000 XLEAP-SBS™ Index Primer Kit
20112858
Reagents to utilize custom index primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge and MiSeq i100 series cartridges. This kit is sufficient for 10 custom index primer uses.
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NextSeq™ 1000/2000 XLEAP-SBS™ Read Primer Kit
20112859
Reagents to utilize custom read primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge and MiSeq i100 series cartridges. This kit is sufficient for 10 custom read primer uses.
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MiSeq i100 Series 5M Reagent Kit (300 cycles)
20126565
This reagent kit provides a 5M flow cell and a reagent consumable for 300 cycles run on MiSeq i100 and MiSeq i100 Plus systems
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MiSeq i100 Series 5M Reagent Kit (600 cycles)
20126566
This reagent kit provides a 5M flow cell and a reagent consumable for 600 cycles run on MiSeq i100 and MiSeq i100 Plus systems
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MiSeq i100 Series 25M Reagent Kit (100 cycles)
20126567
This reagent kit provides a 25M flow cell and a reagent consumable for 100 cycles run on MiSeq i100 and MiSeq i100 Plus systems
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MiSeq i100 Series 25M Reagent Kit (300 cycles)
20126568
This reagent kit provides a 25M flow cell and a reagent consumable for 300 cycles run on MiSeq i100 and MiSeq i100 Plus systems
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MiSeq i100 Series 25M Reagent Kit (600 cycles)
20115696
This reagent kit provides a 25M flow cell and a reagent consumable for 600 cycles run on MiSeq i100 and MiSeq i100 Plus systems
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PhiX Control v3
FC-110-3001
Kitted DNA control for the Illumina sequencing platform. Compatible with Single and Paired End reads up to 150 base pairs. (10ul of 10nM template solution)
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MiSeq i100 Series Reagent Kits are required to use the MiSeq i100 Series Systems. You’ll also need a library prep kit. The PhiX Control v3 is recommended for use as a sequencing control.
The MiSeq i100 Series offers breakthrough advancements in system design, XLEAP-SBS chemistry, and integrated data analysis. It delivers operational simplicity, high data accuracy, and exceptional speed, generating results up to 4× faster. It has 4× higher output, 4× faster turnaround time powered by XLEAP-SBS chemistry, index-first sequencing, onboard DRAGEN secondary analysis, and room-temperature ship-and-store consumables.
The MiSeq i100 System (available in 2025) is a lower cost instrument that runs 5M and 25M flow cells to support lower data output and sample throughputs.
The MiSeq i100 Plus System (available now) accesses a wider range of throughputs with 5M, 25M, 50M, and 100M flow cells for more sequencing per sample and larger studies. The 5M and 25M flow cells are available now. The 50M and 100M flow cells will be available H2 2025 for the MiSeq i100 Plus System only.
The MiSeq i100 Series features index-first sequencing, which enables early demultiplexing of run data. This allows users to obtain a preview of sample representation prior to the completion of a run, enabling subsequent run planning as needed. Index-first sequencing is the default on the MiSeq i100 Series; however, standard sequencing is also available.
Support documentation, product information, tools, and training are located at MiSeq i100 Series Support Resources. You can also contact Illumina Technical Support at techsupport@illumina.com.
The MiSeq i100 Series analysis options include:
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Unique dual (UD) indexes enable preparation of high-quality libraries that can be multiplexed for increased throughput and cost-saving benefits.
A complete targeted resequencing solution for a wide range of applications, with fast integrated workflow and options for custom and fixed enrichment panels.
Nextera DNA Indexes enable preparation of high-quality libraries that can be multiplexed for increased throughput.
Reagents for the iSeq 100 System feature a ready-to-use cartridge with one-channel SBS chemistry and CMOS technology for simple, low-cost sequencing.
Targeted sequencing panel that assesses germline mutations across 113 genes and 125 SNPs associated with genetic cancer risk predisposition.
Library prep and enrichment kit that delivers highly sensitive, comprehensive respiratory pathogen identification and antimicrobial resistance insights.
A library prep and enrichment kit that enables sensitive, culture-free detection and analysis of common and underrecognized uropathogens and AMR genes.
A streamlined workflow offering highly sensitive detection and characterization of common respiratory viruses, including SARS-CoV-2 strains.
A flexible and streamlined NGS library prep kit that enables various public health surveillance and microbial research applications.
Looking for more? Reach out to talk to a specialist about the MiSeq i100 Series.
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