Healthcare-associated infection surveillance
See how NGS is used to identify the genetic makeup of pathogens for more precise identification and tracking of outbreaks and transmission routes than ever before.
Whole-genome sequencing for comprehensive analysis of microbes
Whole-genome sequencing of microbial isolates is an important tool for mapping genomes of novel organisms, finishing genomes of known organisms, or comparing genomes across multiple samples. Sequencing entire microbial genomes is important for generating accurate reference genomes, for microbial identification, and for other comparative genomic studies. 1-6
Unlike traditional methods, NGS-based microbial genome sequencing doesn’t rely on labor-intensive cloning steps, saving time and simplifying the workflow. NGS can identify low-frequency variants and genome rearrangements that may be missed or are too expensive to identify using other methods.
De novo whole-genome sequencing involves assembling a genome without the use of a genomic reference and is often used to sequence novel microbial genomes.7 Illumina sequencers provide unparalleled raw read accuracy and read depth for high-quality draft and microbial whole-genome assemblies.8
Microbial whole-genome resequencing involves sequencing the entire genome of a bacteria, virus, or other microbe, and comparing the sequence to that of a known reference. Generating rapid and accurate microbial reference genome sequence information is critical for detecting low frequency mutations, finding key deletions and insertions, and discovering other genetic changes among microbial strains. 9-12
Take the guesswork out of your next workflow. The NGS Workflow Finder provides personalized solution recommendations and resources so you can sequence with confidence.
A fast, integrated workflow for preparing libraries for use in sequencing applications such as microbial whole-genome sequencing.
NGS technology in an easy-to-use, inexpensive ecosystem for microbial whole-genome sequencing applications.
Simple, accessible benchtop sequencing for microbial whole-genome sequencing applications.
A powerful and compact system ideal for rapid, cost-effective microbial whole-genome sequencing applications.
The SRST2 app reports the presence of STs (sequence types) from a MLST database and/or reference genes from a sequence database of virulence genes, resistance genes, and plasmid replicons.
Outbreak detection is possible through comprehensive isolate discrimination and characterization through microbial WGS.
Interested in learning more about microbial whole-genome sequencing?
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