NovaSeq 6000Dx Instrument

The future of clinical genomics is here

Welcome to a new era for clinical labs. The NovaSeq 6000Dx is our first IVD-compliant high-throughput sequencing instrument for the clinical lab. Push the boundaries of clinical genomics with scalable throughput and improved economics like never before.

NovaSeq 6000Dx instrument
Thumbnail

Scalable throughput in an FDA-regulated, CE-marked sequencing platform

Scientist in clinical laboratory
Conduct in vitro diagnostics and clinical research on a single instrument

The NovaSeq 6000Dx features dual modes for IVD and RUO applications, opening new possibilities across a range of sample types, sequencing methods, and clinical research applications.

Illumina DRAGEN server
Integrated data analysis with DRAGEN

The NovaSeq 6000Dx system includes a DRAGEN Server paired with the sequencer, enabling streamlined and accurate data analysis. The intuitive software interface makes it easy to maximize your analysis efficiency.

NovaSeq 6000Dx user interface
Intuitive, streamlined user interface

Plan and stagger your sequencing runs and analysis using Illumina Run Manager. Monitor run progress, control instrument, and user management from the instrument or remotely. Illumina Run Manager also helps ensure data security and privacy.

NovaSeq 6000Dx flow cells
Flexible targeted sequencing

NovaSeq 6000Dx is compatible with Illumina DNA Prep with Enrichment Dx, a rapid library preparation and enrichment solution that works with fixed and custom panels for increased flexibility. Combined with the integrated DRAGEN for Illumina DNA Prep with Enrichment Dx App, the NovaSeq 6000Dx enables a high-throughput clinical testing workflow.

Get a Tour

NovaSeq 6000Dx instrument specifications 

System specifications (IVD mode)

  • Output range
    S2 Flow Cell: ≥ 1 Tb
    S4 Flow Cell: ≥ 3 Tb
  • Paired end reads per flow cell
    S2 Flow Cell: ≥ 6.67 B S4 Flow Cell: ≥ 20 B
  • Max read length
    2 × 150 bp
  • Run time
    S2: ≤ 40 hours
    S4: ≤ 45 hours

Simplified sequencing workflow

The NovaSeq 6000Dx instrument is part of an integrated, three-step workflow that includes library preparation, high-throughput sequencing in either IVD or RUO modes, and accurate and efficient data secondary analysis with a paired DRAGEN Server.

1
Prepare libraries

Prepare libraries with a growing selection of high-performance library prep kits, and manage your workflow with LIMS (Laboratory Information Management System).

2
Sequence

Sequence with ready-to-use reagents featuring Illumina sequencing by synthesis chemistry for consistent, reliable data.

3
Analyze data

Leverage the paired DRAGEN server and menu of DRAGEN applications for accurate and efficient secondary analysis.

Flexible for a variety of applications

From an integrated, unified interface, users can easily switch between RUO and IVD modes to run the NovaSeq 6000Dx instrument, offering the flexibility to perform IVD testing, translational development, and clinical research on a single platform.

NovaSeq 6000Dx in lab with scientist
Reagent Rental Program

Illumina offers a reagent rental program to help labs maximize their budgets while staying competitive. This program is designed to fit the way customers budget for capital purchases and covers all products and countries where we sell direct.

Optimize your lab performance

Instrument reliability is crucial for your lab. To maximize uptime, we offer remote monitoring and proactive support to detect potential risks before your run. We offer troubleshooting, maintenance, real-time updates, and more—all in one personal dashboard.

Compare with NextSeq 550Dx & NovaSeq 6000

 

NovaSeq 6000Dx


NextSeq 550Dx

NovaSeq 6000

Regulatory Status For In Vitro Diagnostic Use For In Vitro Diagnostic Use For Research Use Only
Key Applications – Diagnostic Mode

Targeted enrichment (Illumina DNA Prep with Enrichment Dx)

IVD assay development

Future Illumina assays

Future partner assays

Targeted enrichment (Illumina DNA Prep with Enrichment Dx)

TruSight Oncology Comprehensive (EU)

VeriSeq NIPT

Future Illumina assays

Future partner assays

Not available
Key Applications – Research Mode

Whole-genome sequencing

Targeted DNA sequencing (high-throughput exome and large custom enrichment panels)

Whole-transcriptome sequencing

Targeted RNA sequencing (exome, custom enrichment panels)

Methylation sequencing

Explore research applications

Targeted DNA sequencing (lower throughput exome, smaller custom enrichment panels and amplicon)

Whole-transcriptome sequencing

Targeted RNA sequencing (exome, custom enrichment and amplicon panels, mRNA-seq and gene expression profiling)

Cytogenomic arrays

Explore research applications

Whole-genome sequencing

Whole-genome de novo sequencing

Targeted DNA sequencing (high-throughput exome and large custom enrichment panels)

Whole-transcriptome sequencing

Targeted RNA sequencing (exome, custom enrichment panels)

Methylation sequencing

Explore research applications
System Software Integrated ecosystem including DRAGEN Server and Illumina Run Manager Local Run Manager BaseSpace Sequence Hub
Analysis

Analysis apps for Illumina DNA Prep with Enrichment (RUO and Dx) available with a graphical user interface on the paired DRAGEN server.

Analysis app for Germline (RUO) available with graphical user interface on paired DRAGEN server. BCL Convert app (RUO) available with graphical user interface on paired DRAGEN server.

Additional RUO applications enabled through BaseSpace Sequence Hub with a stand-alone DRAGEN server purchased separately.

Local Run Manager

Illumina Connected Analytics

BaseSpace Sequence Hub

DRAGEN Server

Popular products

NovaSeq 6000Dx Library Tube

Peform clinical applications using the IVD-compliant NovaSeq 6000Dx Library Tube for use with the NovaSeq 6000Dx System.

NovaSeq 6000Dx S2 Reagent Kits

This IVD-compliant reagent kit provides one NovaSeq S2 flow cell (with 2-lanes) and reagent consumables to support a single flow cell 300 cycles run on the NovaSeq 6000Dx.

NovaSeq 6000Dx S4 Reagent Kits

This IVD-compliant reagent kit provides one NovaSeq S4 flow cell (with 4-lanes) and reagent consumables to support a single flow cell 300 cycles run on the NovaSeq 6000Dx.

Intended Use for the NovaSeq 6000Dx Instrument

United States:

The NovaSeq 6000Dx instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue when used with in vitro (IVD) diagnostic assays. The NovaSeq 6000Dx instrument is not intended for whole-genome or de novo sequencing. The NovaSeq 6000Dx instrument is intended for use with specific registered, certified, or approved IVD reagents and analytical software.

European Union/other:

The NovaSeq 6000Dx instrument is intended for sequencing of DNA libraries when used with in vitro diagnostic (IVD) assays. The NovaSeq 6000Dx instrument is intended for use with specific registered, certified, or approved IVD reagents and analytical software.

Ready to transform your clinical lab?

With dual operating modes, scalable throughput, and broad flexibility, the NovaSeq 6000Dx will usher in a new era for your clinical laboratory. Contact us today to discuss how the instrument can accelerate your diagnostic and clinical research pursuits.