Flow Cell Type | Read Length | Output | Reads Per Run(‡) | Run Time | Data Quality (¶) |
---|---|---|---|---|---|
S2 | 2 x 150 bp | ≥ 1 Tb | ≥ 6.67 B | ≤ 40 hours | ≥ 85% Q30 |
S4 | 2 x 150 bp | ≥ 3 Tb | ≥ 20 B | ≤ 45 hours | ≥ 85% Q30 |
IVD-compliant high-throughput sequencing for the clinical lab
Take a closer look at the output, run time, read length, sample throughput, and other NovaSeq 6000Dx specifications
Flow Cell Type | Read Length | Output | Reads Per Run(‡) | Run Time | Data Quality (¶) |
---|---|---|---|---|---|
S2 | 2 x 150 bp | ≥ 1 Tb | ≥ 6.67 B | ≤ 40 hours | ≥ 85% Q30 |
S4 | 2 x 150 bp | ≥ 3 Tb | ≥ 20 B | ≤ 45 hours | ≥ 85% Q30 |
• Libraries generated with Illumina DNA Prep with Enrichment Dx.
† For performance parameters in RUO mode, see NovaSeq 6000 System specifications.
‡ Paired-end reads per flow cell.
¶ A quality score of Q30 corresponds to an error rate of 1 in 1000 bases called.
Germline variant calling | Somatic variant calling | |
---|---|---|
SNV Positive Percent Agreement (%) | ≥ 99.8% | ≥ 99.8% |
Insertion PPA (%) | ≥ 97.9% | 100% |
Deletion PPA (%) | ≥ 96.9% | 100% |
Negative Percent Agreement (%) | > 99.9% | > 99.9% |
Overall Percent Agreement (%) | > 99.9% | > 99.9% |
*Variant calling performance results obtained using a representative assay designed to query a variety of genes covering approximately 2 million bases across 23 different chromosomes, and containing wide GC content (20% to 86%), insertions (up to 18 base pairs), and deletions (up to 21 base pairs).
NovaSeq 6000Dx instrument specifications
Includes 3.5 kg (7.8 lb) for leak tray and 0.9 kg (2 lb) for keyboard and mouse
Specifications shown here are for IVD use. View Research Mode only specs, applicable only when running the NovaSeq 6000Dx instrument in Research Mode.
Accurate and efficient data analysis technology
Each NovaSeq 6000Dx instrument comes with a paired dedicated DRAGEN Server and included license for accelerated secondary analysis. With significant advancements in hardware acceleration, lossless compression, and technology innovation, DRAGEN is setting new standards for accuracy and increased genome coverage to deliver actionable insights faster.
Run management technology
Illumina Run Manager for NovaSeq 6000Dx is fully integrated with the sequencing system. Accessed either on-instrument or remotely, this intuitive interface enables control of user and instrument management, application configuration, run setup, and secondary analysis modules on the DRAGEN Server. Plan and start your sequencing runs in IVD and RUO Modes, track libraries with audit trails, and monitor run progress.
Sequencing by synthesis technology
NovaSeq 6000Dx leverages proven Illumina sequencing by synthesis (SBS) technology to deliver accurate data and robust performance. SBS chemistry uses reversible-terminator fluorescently labeled nucleotides. This method detects single bases as they are incorporated into growing DNA strands, reading billions of sequences in parallel.
Patterned flow cell technology
NovaSeq 6000Dx incorporates patterned flow cell technology to generate a high level of throughput for a broad range of sequencing applications. Patterned flow cells contain billions of nanowells at fixed locations, a design that provides even spacing of sequencing clusters. This delivers significant increases in sequencing reads and total output of the system.
The NovaSeq 6000Dx instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue when used with in vitro (IVD) diagnostic assays. The NovaSeq 6000Dx instrument is not intended for whole-genome or de novo sequencing. The NovaSeq 6000Dx instrument is intended for use with specific registered, certified, or approved IVD reagents and analytical software.
The NovaSeq 6000Dx instrument is intended for sequencing of DNA libraries when used with in vitro diagnostic (IVD) assays. The NovaSeq 6000Dx instrument is intended for use with specific registered, certified, or approved IVD reagents and analytical software.
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