NovaSeq 6000Dx instrument applications and methods

NovaSeq 6000Dx instrument applications

Dual modes of operation enable both IVD testing and clinical research, opening new possibilities across a range of sample types, sequencing methods, and applications

NovaSeq 6000 flow cell

Key diagnostic applications

TruSight Whole Genome offers an EU IVDR 2017/746-compliant and comprehensive, DNA-to-variant call format (VCF) workflow solution for clinical WGS. Only available in select locations (see availability on product page).

Application note:
Validating TruSight Whole Genome

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Prep
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Sequence
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Analyze
TruSight Whole Genome Analysis Application via DRAGEN server with Illumina Run Manager

The DRAGEN server features an integrated FPGA chip for accurate, ultrarapid NGS secondary data analysis with DRAGEN for NovaSeq 6000Dx and a dedicated analysis module for TruSight Whole Genome.

Illumina DNA Prep with Enrichment Dx is a fast, integrated workflow for a wide range of applications. It supports a broad DNA input range and multiple sample types including blood, genomic DNA, and formalin-fixed, paraffin-embedded (FFPE) tissue..

Application note:
Target enrichment on the NovaSeq 6000Dx Instrument

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Sequence
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DRAGEN for Illumina DNA Prep with Enrichment Dx for NovaSeq 6000Dx

The DRAGEN server features an integrated FPGA chip for accurate, ultrarapid NGS secondary data analysis with DRAGEN for NovaSeq 6000Dx and a dedicated analysis module for Illumina DNA Prep with Enrichment Dx.

Key clinical research applications (in research mode)

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Sequence
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BCL Convert

Utilize the NovaSeq 6000Dx DRAGEN server in RUO mode to convert BCL files to FASTQ.

Use converted FASTQ files as the input to any custom secondary analysis for fewer user touchpoints and improved workflow efficiency.

Reduce storage and operational costs by up to 5x with lossless FASTQ ORA compression.

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Prep
2
Sequence
3
Analyze

Paired DRAGEN server for NovaSeq 6000Dx

Leverage the Illumina DRAGEN for Illumina DNA Prep with Enrichment (IDPE) App in RUO mode for accurate and efficient secondary analysis.

BCL Convert

Utilize the NovaSeq 6000Dx DRAGEN server in RUO mode to convert BCL files to FASTQ.

Use converted FASTQ files as the input to any custom secondary analysis for fewer user touchpoints and improved workflow efficiency.

Reduce storage and operational costs by up to 5x with lossless FASTQ ORA compression.

1
Prep
2
Sequence
3
Analyze

Stand-alone DRAGEN server or Illumina Connected Analytics (ICA)

Rapid variant calling algorithm powered by DRAGEN pipelines, available locally with separate, stand-alone DRAGEN server. Secure, scalable, cloud-based variant calling is also available with DRAGEN pipelines on Illumina Connected Analytics (ICA), featuring auto-launch capability.

Intended Use for the NovaSeq 6000Dx Instrument

United States:

The NovaSeq 6000Dx instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue when used with in vitro (IVD) diagnostic assays. The NovaSeq 6000Dx instrument is not intended for whole-genome or de novo sequencing. The NovaSeq 6000Dx instrument is intended for use with specific registered, certified, or approved IVD reagents and analytical software.

European Union/other:

The NovaSeq 6000Dx instrument is intended for sequencing of DNA libraries when used with in vitro diagnostic (IVD) assays. The NovaSeq 6000Dx instrument is intended for use with specific registered, certified, or approved IVD reagents and analytical software.